microRNA-148a-3p inhibited the proliferation and epithelial–mesenchymal transition progression of non-small-cell lung cancer via modulating Ras/MAPK/Erk signaling

Son of sevenless (SOS) is one of the guanine nucleotide exchange factors that can regulate the mitogen-activated protein kinase/extracellular signal regulated kinase signal pathway via controlling the activation of Ras. microRNAs are key regulon of gene expression and would be treated as tumor biomarkers or therapeutic targets. In this study, we find that miR-148a-3p acts as a tumor-suppressor in the development and progression of non-small-cell lung cancer (NSCLC). miR-148a-3p inhibits NSCLC cells proliferation and epithelial–mesenchymal transition by reducing the expression of SOS2, which refers Ras activating. Our findings demonstrate that the miR-148a-3p may play a significant role in NSCLC including the kind of lung cancer with K-Ras gene mutation, and it exerted the tumor inhibitor function by targeting SOS2. Because of that, miR-148a-3p and SOS2 may be an efficient target in developing more useful therapies against NSCLC.     

Protective effect of ulinastatin on hepatic ischemia reperfusion injury through autophagy activation in Chang liver cells

This study aimed to investigate the protective effect of ulinastatin in hepatic ischemia-reperfusion progress, involving its association with the role of autophagy during hypoxia-induced hypoxia-reoxygenation injury in vitro. The model of hepatic hypoxia/reoxygenation (H/R) injury in Chang liver cells was established. After treatment with ulinastatin at the doses of 10, 100, and 1000 U/mL in H/R liver cells, the cell proliferation was significantly increased, morphological damage was reduced, and the cell apoptosis rate was decreased. The protein levels of antiapoptotic myeloid cell leukemia-1 (Mcl-1) and caspase-3 were upregulated, and C-PARP protein was downregulated. Meanwhile, ulinastatin led to an increase in the messenger RNA and protein levels of autophagy maker Unc-like kinase 1 (ULK1), Beclin-1, and microtubule-associated protein 1 light chain 3 (LC-3) and a decrease in p62. Then, 3-methyladenine (3-MA), an inhibitor of autophagy, made morphological damage and cell apoptosis worsen in ulinastatin-treated H/R liver cells. And the expression levels of caspase-3, C-PARP, p62, Beclin-1, and LC-3, proteins were also reversed by 3-MA. Taken together, our results demonstrate that ulinastatin inhibited the hepatic H/R injury in Chang liver cells, which was, to some extent, related to the autophagy activation.     

甜菜坏死黄脉病毒外壳蛋白基因在甜菜转基因植株中的表达

甜菜坏死黄脉病毒(Beet Necrotic Yellow Vein Virus,BNYVV)是一种由甜菜多粘菌(Polymyxo be tae)传播的多分体植物病毒．基因组由4～5条单链正意RNA构成^[1]。60年代末,由Tamada首次报道^[2],这种病毒可对甜菜造成严重危害,侵染甜菜后产生丛根症状(Rhizomania),并导致甜菜产量和含糖 量的大幅度下降。除欧洲、北美及日本的严重发生以外,我国自70年代以来在东北、内蒙古及西北许多省区也有大量甜菜丛根病的发生报道^[3]。由于尚无有效药剂及措施用于甜菜丛根病或病毒传播介体的防治．在我国也无法采用大面积轮作作为防治手段,所以目前在世界各地及我国上述地区甜菜丛根病的发病面积逐年扩展,对甜菜生产和制糖业造成直接威胁。针对这一情况．本文报道了含有甜菜坏死黄脉病毒外壳蛋白基因的甜菜植株的转化再生工作,以期在甜菜亲本育种中获得新的抗性材料．为抗病毒品种的培育打下基础。     

Investigation on the interactions between pirarubicin and phospholipids

Differential scanning calorimetry (DSC), Fourier transform infrared (FTIR) spectroscopy and quantum calculation based on molecular modeling were applied to investigate the interaction between pirarubicin (THP), an anthracycline antibiotic frequently used in chemotherapy, and zwitterionic distearoylphosphatidylcholine (DSPC) or anionic distearoylphosphatidylglycerol (DSPG). DSC and FTIR studies suggested that DSPG bilayers were less perturbed by THP than those of DSPC, and this might be due to the strong interactions between NH₃⁺ of THP and the phosphate (PO₂⁻) group in the polar head of DSPG, which limit the further access of THP into its bilayers. Quantum calculation results based on molecular modeling could further confirm the DSC and FTIR conclusions. Meanwhile, it could well translate the calorimetric and spectroscopic phenomena into the underlying physical knowledge. Interactions between THP and phospholipids can play a critical role in the liposomal drug delivery system, especially in the safety mechanism elucidation and rational formulation design.     

基于微信公众平台的文献定制服务

文献学习是科研人员跟踪领域进展,思考课题发展的必要途径。为了解决移动端跟踪文献的繁琐问题,本文借助移动互联网即时性、便捷性、个性化等特性,开发了基于微信公众平台的文献定制服务。该服务通过解析用户输入,动态匹配自构建的期刊名表,索引Pub Med数据库,实现期刊个性化订阅、文献查询、影响因子查询等便捷功能。对于提高科研人员的文献学习效率,降低追踪文献的时间成本具有较大的价值。     

自然保护区设计的主要原理和方法

本文回顾了自然保护区设计的主要原理和方法。建立自然保护区的目的是防止物种绝灭和生物多样性消失。岛屿生物地理学的“平衡理论”的设计原则未涉及到物种绝灭的中心问题———种群生存力,所以该理论对保护实践贡献较小。种群生存力分析和最小可存活种群的理论及模型用于物种绝灭问题的研究,同时关键种的生存力用于确定生态系统的生存力,因而该理论和模型成为自然保护区设计的理论基础。保护评估方法已应用于自然保护区的选择和检验中。走廊辩论取代了ＳＬＯＳＳ辩论,成为自然保护区设计争论的热点     

套式聚合酶链反应在HIV-1检测中的应用

根据HIV-1 gag.pol和env基因序列设计了套式聚合酶链反应(nesled polymerasechain reaction,nPCR)引物。将外周血单个核细胞裂解液先用外侧引物扩增,其产物再经内侧引物扩增,直接走凝胶电泳判定结果。nPCR的敏感性。较常规PCR高100～1000倍,可检测到0.5fg质粒DNA和50μ1HIV-1感染者外周血样中的HIV基因。用本法检测63名HIV-1感染者全为阳性,而61名健康人和可疑者均为阴性,后者经追踪检测抗体排除了HIV-1感染。实验证明,nPCR是一种敏感性极高、特异性很强、操作简便易行的HIV-1基因检测技术。它已经在早期诊断和外周血病毒含量检测中发挥了重要作用,而且有着更广阔的应用前景。     

人淋巴毒素基因在中国仓鼠细胞中表达

将人淋巴毒素(HuLT)基因插入哺乳动物细胞表达载体质粒p91023,经磷酸钙-DNA共沉淀法转入中国仓鼠二氢叶酸还原酶缺陷型(CHO-DHFRˉ)细胞,获得了DIIFR 细胞克隆。RNA点渍杂交分析和MTT染料还原测定淋巴毒素的生物活性,均证明HuLT基因在DHFR 细胞里巳被转录和转译,并被分泌到细胞外。其杀伤靶细胞——L929细胞的生物活性不低于每毫升培养液200单位。     

舟山岛自然栖息地的变化及其对兽类物种绝灭影响的初步研究

舟山岛自然栖息地和物种受到人类活动的强烈干扰．占总面积５６．３％的自然栖息地已经消失,自然栖息地已破碎成许多碎片,碎片面积不断变小,碎片数量在增加．自然栖息地的质量发生了很大变化．自然栖息地的易破碎带与其地理特征有关,破碎易发生于被开垦和开发的地带．由于自然栖息地面积损失、破碎、质量下降和过度捕杀,已有５种兽绝灭,３种兽正走向绝灭,绝灭速度高于临近大陆．人口增长是舟山岛自然栖息地损失、破碎、质量下降和物种绝灭的根本原因．本文还讨论了保护自然栖息地和物种多样性的一些措施和原则．