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161.
Turkey is one of the most important mulberry fruit producers in the world. In particular, in Eastern and Inner part of Turkey,
mulberry fruits are processed into several products such as `Mulberry Pekmez', `Mulberry Pestil', `Mulberry Kome' etc. Therefore,
mulberry fruits give higher economic returns thus have greater employment potentials in Turkey. In order to improve the yield
and fruit quality through breeding, the genetic variations and relationships among 15 selected white mulberry genotypes (Morus alba L.) were evaluated using Random Amplified Polymorphic DNA (RAPD). A total of 101 DNA markers were generated by 16 random
primers, with an average of 6.3 easily detectable markers per primer. Several RAPD markers showed unique patterns of mean
frequency that differed among the white mulberry genotypes. The distance matrix showed that the highest genetic distance (0.902)
was between EMS13 and EMS8 and the least (0.413) was between EMS12 and EMS15. According to the results, RAPD analysis can
be used for the characterization and grouping of mulberry genotypes. The genetically divergent genotypes identified from this
study would be of much use in the future breeding program. 相似文献
162.
Kurt Nezahat Ozgeris Fatma B. Ucuz Ilknur Bayraktutan Zafer Kocak Yilmaz Kubra Demirdogen Esen Yildirim Cayir Atilla 《Biochemistry. Biokhimii?a》2022,87(6):559-565
Biochemistry (Moscow) - Early detection of cognitive developmental delay (CDD) and autism spectrum disorder (ASD) is challenging, despite the numerous scientific studies conducted and different... 相似文献
163.
Deniz Yildirim Sevgi Ates Birgul Yazici S. Seyhan Tukel 《Biocatalysis and Biotransformation》2019,37(3):210-223
In this study, Rhizomucor miehei lipase (RML) was immobilized on the hexagonally-ordered nanoporous aluminium oxide membranes (RML-Al2O3-NP) by adsorption and as protein-coated microcrystals (RML-PCMCs) by simultaneously precipitating RML on micron-sized potassium sulfate crystals (K2SO4) in pre-chilled acetone. The hydrolytic activities of immobilized lipase preparations were investigated in terms of p-nitrophenyl palmitate hydrolysis and their esterification activities were examined for the synthesis of some aroma esters such as butyl acetate, isoamyl acetate, hexyl acetate, heptyl acetate, and geranyl acetate. The immobilization yields were 33.8 and 25.1%, respectively for RML immobilized on Al2O3-NP membranes and potassium sulfate crystals. The catalytic efficiency ratios of RML-Al2O3-NP and RML-PCMCs were 2.3- and 3.9-fold higher than that of the free lipase, respectively in terms of hydrolytic activity. The free lipase was stabilized as 4.1- and 10.5-fold, respectively at 40 and 50?°C when immobilized on Al2O3-NP. The corresponding stabilization factors were 4.6- and 12.8-fold higher for RML-PCMCs. RML-Al2O3-NP and RML-PCMCs maintained 84 and 86% of their initial hydrolytic activities, respectively after 10 reuses. Of the synthesized aroma esters, the highest yield was obtained for the geranyl acetate. After 4?h reaction time, no geraniol was detected in the preparative-scale (196?g/L) synthesis of geranyl acetate for both the immobilized lipases when the initial geraniol amount, vinyl acetate amount, RML-PCMCs amount, and reaction temperature values were 1?mmol, 3?mmol, 100?mg (or 300?mg RML-Al2O3-NP), and 50?°C, respectively. These results show that the immobilization of R. miehei lipase by adsorption on nanoporous aluminium oxide and as protein-coated microcrystals leads to the obtention of highly stable, catalytically more active, and reusable lipase preparations. 相似文献
164.
The influence of a longer photoperiod on growth parameters of European sea bass Dicentrarchus labrax (Linnaeus, 1758) reared in sea cages
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The aim of this study was to investigate the effects of a long‐term photoperiod (15L : 9D) on both the somatic growth and husbandry parameters of sea bass Dicentrarchus labrax. Experiments were conducted in triplicate in high density polyethylene (HDPE) floating cages with net depths of 14 m and diameters of 20 m. Experiments were performed with approximately 170 000 sea bass with initial average weights of 110.63 ± 3.12 g and 110.26 ± 29.70 g for the experimental and control groups, respectively. Beginning in April 2008, extended artificial lighting was used in the experimental cages for 13 months; the control group received no additional illumination. At the end of the experiments, the average weights of the experimental and control groups were 362.13 ± 7.72 and 310.34 ± 18.15 g, respectively. Specific growth rate (SGR) was relatively high in the experimental group compared to the control fish. Feed conversion ratio (FCR) was 2.02 and 2.21 for the experimental and control groups, respectively, with significant differences also found between these groups (P < 0.05). Moreover, gonadal fish weight in the control group was significantly higher than in the experimental group, with the gonadosomatic index fluctuating within a 1.71‐fold increase. 相似文献
165.
Acka G Sen A Canakci Z Yildiz S Akin A Uzun G Cermik H Yildirim I Kokpinar S 《Physiological research / Academia Scientiarum Bohemoslovaca》2007,56(3):369-373
The aim of the present study was to evaluate the efficiency of combination of hyperbaric oxygen (HBO) and an antioxidant on permanent focal cerebral ischemia. Male Wistar rats underwent permanent middle cerebral artery occlusion (MCAO). Then, animals were randomly assigned to one of four groups: the control group (n=9) received no treatment, HBO group (n=9) was treated for 90 min at 2.5 absolute atmosphere for 3 days, the U-74389G group (n=8) received single U-74389G injection (3 mg/kg), the HBO + U-74389G group (n=8) received both HBO and U-74389G treatments. Treatments were initiated within the first 10 min after MCAO. After 3 days, the infarct volumes in rat brains were measured. The infarct ratios were 25.6+/-6.5 % for the control group, 21.9+/-6.4 % for the HBO group, 15.7+/-5.7 % for U-74389G group and 12.5+/-3.8 % for HBO + U74389G group. The infarct volumes were significantly reduced in rats treated with U-74389G (p<0.05) and combination therapy (p<0.05). HBO failed to reduce infarct volume significantly. We concluded that 1) U-74389G is more beneficial than HBO on permanent MCAO in rats, and 2) a combined therapy failed to significantly improve infarct volume more than either single treatment. 相似文献
166.
The aim of the study was to evaluate the effect of rosiglitazone treatment on islet ghrelin and insulin gene expressions in streptozotocin (STZ)-induced diabetic rats. Animals were divided into four groups. 1. Intact controls. 2. Rosiglitazone-treated controls. 3. STZ-induced diabetes. 4. Rosiglitazone-treated diabetes. Rosiglitazone was given for 7 days at a dose of 20 mg/kg body weight. Ghrelin and insulin gene expressions were investigated by immunohistochemistry and in situ hybridization. There was no statistically significant difference in body weight between STZ-induced diabetic rats and rosiglitazone-treated diabetic rats during the experimental period. Furthermore, there were no significant differences in blood glucose levels and insulin immunoreactive cell numbers between STZ-induced diabetic rats and rosiglitazone-treated diabetic rats. There was a tendency towards a reduction of ghrelin gene expression in diabetic animals compared with intact controls. We found, in addition, that ghrelin immunoreactive and ghrelin mRNA expressing cells were frequent in the epithelial lining of the ducts suggesting ductal epithelium might be the source of the regenerating islet ghrelin cells, as is known for other islet cells. The results show that short-term rosiglitazone pretreatment had no significant effect on ghrelin and insulin gene expressions. 相似文献
167.
Nuno Maia Sven Potelle Hamide Yildirim Sandrine Duvet Shyam K. Akula Celine Schulz Elsa Wiame Alexander Gheldof Katherine OKane Abbe Lai Karen Sermon Maïa Proisy Philippe Loget Tania Atti-Bitach Chlo Quelin Ana Maria Fortuna Ana Rita Soares Arjan P.M. de Brouwer Emile Van Schaftingen Marie-Ccile Nassogne Christopher A. Walsh Katrien Stouffs Paula Jorge Anna C. Jansen Franois Foulquier 《American journal of human genetics》2022,109(2):345-360
168.
Alice Lu-Culligan Alexandra Tabachnikova Eddy Prez-Then Maria Tokuyama Hannah J. Lee Carolina Lucas Valter Silva Monteiro Marija Miric Vivian Brache Leila Cochon M. Catherine Muenker Subhasis Mohanty Jiefang Huang Insoo Kang Charles Dela Cruz Shelli Farhadian Melissa Campbell Inci Yildirim Albert C. Shaw Shuangge Ma Sten H. Vermund Albert I. Ko Saad B. Omer Akiko Iwasaki 《PLoS biology》2022,20(5)
The impact of Coronavirus Disease 2019 (COVID-19) mRNA vaccination on pregnancy and fertility has become a major topic of public interest. We investigated 2 of the most widely propagated claims to determine (1) whether COVID-19 mRNA vaccination of mice during early pregnancy is associated with an increased incidence of birth defects or growth abnormalities; and (2) whether COVID-19 mRNA-vaccinated human volunteers exhibit elevated levels of antibodies to the human placental protein syncytin-1. Using a mouse model, we found that intramuscular COVID-19 mRNA vaccination during early pregnancy at gestational age E7.5 did not lead to differences in fetal size by crown-rump length or weight at term, nor did we observe any gross birth defects. In contrast, injection of the TLR3 agonist and double-stranded RNA mimic polyinosinic-polycytidylic acid, or poly(I:C), impacted growth in utero leading to reduced fetal size. No overt maternal illness following either vaccination or poly(I:C) exposure was observed. We also found that term fetuses from these murine pregnancies vaccinated prior to the formation of the definitive placenta exhibit high circulating levels of anti-spike and anti-receptor-binding domain (anti-RBD) antibodies to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) consistent with maternal antibody status, indicating transplacental transfer in the later stages of pregnancy after early immunization. Finally, we did not detect increased levels of circulating anti-syncytin-1 antibodies in a cohort of COVID-19 vaccinated adults compared to unvaccinated adults by ELISA. Our findings contradict popular claims associating COVID-19 mRNA vaccination with infertility and adverse neonatal outcomes.The impact of COVID-19 mRNA vaccination on pregnancy and fertility has become a major topic of public interest. This study shows that after inoculation of pregnant mice with COVID mRNA vaccines, no birth defects or growth restrictions were found, and no induction of anti-syncytin-1 antibodies was detected in a longitudinal human cohort compared to unvaccinated volunteers. 相似文献
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