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951.
Here we describe the in vitro polymerization of actin from maize (Zea mays) pollen. The purified actin from maize pollen reported in our previous paper (X. Liu, L.F. Yen [1992] Plant Physiol 99: 1151-1155) is biologically active. In the presence of ATP, KCl, and MgCl2 the purified pollen actin polymerized into filaments. During polymerization the spectra of absorbance at 232 nm increased gradually. Polymerization of pollen actin was evidently accompanied by an increase in viscosity of the pollen actin solution. Also, the specific viscosity of pollen F-actin increased in a concentration-dependent manner. The ultraviolet difference spectrum of pollen actin is very similar to that of rabbit muscle actin. The activity of myosin ATPase from rabbit muscle was activated 7-fold by the polymerized pollen actin (F-actin). The actin filaments were visualized under the electron microscope as doubly wound strands of 7 nm diameter. If cytochalasin B was added before staining, no actin filaments were observed. When actin filaments were treated with rabbit heavy meromyosin, the actin filaments were decorated with an arrowhead structure. These results imply that there is much similarity between pollen and muscle actin. 相似文献
952.
953.
954.
Greenhouse hydroponic experiments were conducted using Cd-sensitive (Xiushui63) and tolerant (Bing97252) rice genotypes to
evaluate genotypic differences in response of photosynthesis and phytochelatins to Cd toxicity in the presence of exogenous
glutathione (GSH). Plant height, chlorophyll content, net photosynthetic rate (Pn), and biomass decreased in 5 and 50 μM Cd
treatments, and Cd-sensitive genotype showed more severe reduction than the tolerant one. Cadmium stress caused decrease in
maximal photochemical efficiency of PSII (Fv/Fm) and effective PSII quantum yield [Y(II)] and increase in quantum yield of
regulated energy dissipation [Y(NPQ)], with changes in Cd-sensitive genotype being more evident. Cadmium-induced phytochelatins
(PCs), GSH, and cysteine accumulation was observed in roots of both genotypes, with markedly higher level in PCs and GSH on
day 5 in Bing97252 compared with that measured in Xiushui63. Exogenous GSH significantly alleviated growth inhibition in Xiushui63
under 5 μM Cd and in both genotypes in 50 μM Cd. External GSH significantly increased chlorophyll content, Pn, Fv/Fm, and
Y(II) of plants exposed to Cd, but decreased Y(NPQ) and the coefficient of non-photochemical quenching (qN). GSH addition
significantly increased root GSH content in plants under Cd exposure (except day 5 of 50 μM Cd) and induced up-regulation
in PCs of 5 μM-Cd-treated Bing97252 throughout the 15-day and Xiushui63 of 5-day exposure. The results suggest that genotypic
difference in the tolerance to Cd stress was positively linked to the capacity in elevation of GSH and PCs, and that alleviation
of Cd toxicity by GSH is related to significant improvement in chlorophyll content, photosynthetic performance, and root GSH
levels. 相似文献
955.
Zhou LZ Ruan MM Cai QF Liu GM Sun LC Su WJ Cao MJ 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2012,161(3):247-254
A trypsin was purified from the hepatopancreas of snakehead (Channa argus) by ammonium sulfate fractionation and a series of column chromatographies including DEAE-Sepharose, Sephacryl S-200 HR and Hi-Trap Capto-Q. The molecular mass of the purified trypsin was about 22 kDa, as estimated by SDS-PAGE. The optimum pH and temperature of the purified trypsin were 9.0 and 40 °C, respectively. The trypsin was stable in the pH range of 7.5-9.5 and below 45 °C. The enzymatic activity was strongly inhibited by serine proteinase inhibitors, such as MBTI, Pefabloc SC, PMSF, LBTI and benzamidine. Peptide mass fingerprinting (PMF) of the purified protein obtained 2 peptide fragments with 25 amino acid residues and were 100% identical to the trypsinogen from pufferfish (Takifugu rubripes). The activation energy (Ea) of this enzyme was 24.65 kJ·M− 1. Apparent Km was 1.02 μM and kcat was 148 S− 1 for fluorogenic substrate Boc-Phe-Ser-Arg-MCA. A trypsinogen gene encoding 247 amino acid residues was further cloned on the basis of the sequence obtained from PMF and the conserved site peptide of trypsinogen together with 5′-RACE and 3′-RACE. The deduced amino acid sequence contains a signal peptide of 15 residues and an activation peptide of 9 amino acid residues with a mature protein of 223 residues. The catalytic triad His-64, Asp-107, Ser-201 and 12 Cys residues which may form 6 disulfide bonds were conserved. Compared with the PMF data, only 2 amino acid residues difference were identified, suggesting the cloned trypsinogen is quite possibly the precursor of the purified trypsin. 相似文献
956.
多胚水稻ApⅢ(双13)的胚胎学观察 总被引:6,自引:0,他引:6
对多胚水稻(Oryza sativa L.)ApⅢ的大量成熟颖果、人工萌发的幼苗和开花后3~5 d 的幼嫩颖果进行的整体解剖和显微制片观察表明:ApⅢ的5000粒成熟颖果中,89.0% 含单胚单苗,8.9% 和1.2%分别含双胚双苗和三胚三苗;700多粒幼嫩颖果中,90.0% ~95.0% 含单胚,5.0% ~7.0% 含双胚。因制片的数目有限,未见到含三胚的;在含单胚和多胚颖果中,胚均位于同一胚囊的珠孔端,未见到胚囊以外存在不定胚。根据上述结果,似可以认为ApⅢ单粒颖果的双胚和三胚是由同一胚囊内的卵细胞和1或2个助细胞受精或不受精发育而来的 相似文献
957.
The Crc protein participates in down‐regulation of the Lon gene to promote rhamnolipid production and rhl quorum sensing in Pseudomonas aeruginosa 下载免费PDF全文
958.
厦门凤林红树林湿地大型底栖动物群落 总被引:8,自引:0,他引:8
为摸清厦门集美凤林红树林湿地的大型底栖动物群落结构及其多样性现状,2002年1、4、7和10月在厦门集美凤林红树林区进行大型底栖动物调查,4个季度共获得大型底栖动物42种。生物量优势种是软体动物门的珠带拟蟹守螺(Cerithideacingulata)和节肢动物门的弧边招潮(Ucaarcuata)。密度优势种是软体动物门的短拟沼螺(Assimineabrevicula)和环节动物门的沼蚓(Limnodriloidessp.)。集美凤林红树林区大型底栖动物年平均密度和年平均生物量分别为1,990ind./m2和139.0g/m2。密度的季节变化是:1月>4月>10月>7月,生物量的季节变化是1月>10月>4月>7月。聚类分析和数量分布表明,优势种珠带拟蟹守螺、短拟沼螺、弧边招潮和沼蚓的季节变化各不相同。与2002年10月深圳湾福田红树林区大型底栖动物群落的物种多样性指数平均值(0.56)比较,厦门凤林红树林区的平均值较高(2.66)。文中分析了影响大型底栖动物多样性的环境因素。 相似文献
959.
960.
In this letter, we investigate the extraordinary optical transmission behavior of a flat continuous metal film sandwiched by magnetic plasmonic structures. A new mechanism by utilizing higher order magnetic plasmon resonance is proposed to enhance the transmission. Numerical simulation results show that 80 % electromagnetic energy can be transmitted through the middle 50-nm-thick continuous gold film in near-infrared regime. The excitation of the second-order magnetic plasmons and the propagating surface plasmons, as well as the interaction between them accounts for such a high transmission. The interaction of magnetic plasmons and surface plasmons leads to new hybrid modes, and the coupled oscillator model is introduced to analyze this hybridization. This work extends the application range of higher order magnetic plasmons and may have potential in transparent electrode and electromagnetic energy transfer applications. 相似文献