甘肃省大豆品种生育期组的划分及地理分布研究

甘肃省地域广大,地形复杂,气候多样,大豆品种资源丰富,但甘肃各地大豆品种生育期生态类型并不明确.本试验以25份MG 0～Ⅴ的北美大豆生育期组标准品种为对照,将从甘肃各地收集到的151个大豆品种在甘肃境内5个生态区进行生育期性状鉴定和生育期组划分.结果 表明,甘肃地区种植的大豆品种的生育期组在MG 00和MGⅥ之间.在所...     

miRNAs生物合成过程的调控

miRNAs是一类短的(20-23nt)非编码的单链RNA分子,在转录后水平上通过抑制靶基因的表达而影响生物体的生长、发育以及癌症发生。miRNAs的成熟需要一系列大型蛋白复合体参与的协同加工,目前对miRNAs生物合成过程调控的研究还处于初始阶段。本文主要综述了影响miRNAs的生物合成过程及活性的因素以及相关调控机制。癌症的发生通常伴随着异常的miRNAs表达谱,miRNAs生物合成调控机制的深入研究必将为癌症的基因治疗技术以及新型疗法的开发提供重要的理论基础和指导意义。     

基于CSSL的高密度物理图谱定位水稻分蘖角度QTL

对以籼稻9311为遗传背景携带粳稻日本晴基因组的染色体片段置换系（CSSL）的遗传图谱进行分子标记加密,构建了含250个多态标记的高密度物理图谱。以119个CSSLs为材料,P≤0.001为阈值,筛选到分蘖角度与受体亲本9311差异极显著的10个系。结合物理图谱和代换作图方法,共鉴定出5个分蘖角度QTL,其中qTA11的加性效应表现为增效作用,来源于9311的等位基因;其余4个QTL的加性效应为减效作用,均来源于日本晴的等位基因。qTA6-1和qTA6-2分别被定位于第6染色体RM253–RM527之间的3.55Mb区段和RM3139–RM494的1.65Mb区间;qTA9被定位于第9染色体RM257–RM189之间的3.40Mb区段;qTA10被定位在第10染色体RM222–S10-1之间的2.10Mb区段;qTA11被定位于第11染色体RM1761–RM4504之间的3.30Mb区间。以上研究结果为水稻分蘖角度QTL的精细定位和株型育种提供了依据。     

LncRNA-135528 inhibits tumor progression by up-regulating CXCL10 through the JAK/STAT pathway

Spontaneous tumor regression can be observed in many tumors, however, studies related to the altered expression of lncRNA in spontaneous glioma regression are limited, and the potential contributions of lncRNAs to spontaneous glioma regression remain unknown. To investigate the biological roles of lncRNA-135528 in spontaneous glioma regression. The cDNA fragment of lncRNA-135528 was obtained by rapid-amplification of cDNA ends (RACE) technology and cloned into the plvx-mcmv-zsgreen-puro vector. Additionally, we stably silenced or overexpressed lncRNA-135528 in G422 cells by transfecting with siRNA against lncRNA-135528 or lncRNA-135528 overexpression plasmid. Then, we examined lncRNA-135528 overexpressing and lncRNA-135528 silencing on glioma cells and its effects on CXCL10 and JAK/STAT pathways. The main findings indicated that lncRNA-135528 promoted glioma cell apoptosis, inhibited cell proliferation and arrested cell cycle progression; the up-regulation of lncRNA135528 led to significantly increased CXCL10 levels and the differential expression of mRNA associated with JAK/STAT pathway in glioma cells. lncRNA-135528 can inhibit tumor progression by up-regulating CXCL10 through the JAK/STAT pathway.     

水牛卵巢黄体形成和退化的蛋白质表达谱构建及生物信息学分析

本研究构建了水牛卵巢黄体形成及退化过程的定量蛋白质表达谱,从中寻找与水牛发情周期相关的重要蛋白质。为研究水牛卵巢周期性变化的分子机制提供蛋白质水平的数据支撑,进而为提高水牛繁殖效率奠定实验基础。以水牛排卵后的红体期(corpus hemorrhagicum,CH)、黄体期(corpus luteum,CL)和白体期(corpus fibrosum,CF)的卵巢作为研究对象,利用串联质谱标签(tandem mass tag,TMT)标记结合液质联用((liquid chromatography/mass spectrometry,LC-MS/MS)的定量蛋白质组学技术,采用相关软件对得到的差异表达蛋白质进行生物信息学分析。同时对与水牛发情周期相关的重要差异蛋白质:单核巨噬细胞分化抗原(CD14)、通用转录因子Ⅱ-Ⅰ(GTF2I)、羟基类固醇(17β)脱氢酶1(HSD17B1)、U6 sn RNA相关Sm样蛋白质(LSM1)和纤溶酶原激活物抑制物(SERPINE1)进行了qRT-PCR分析验证。结果显示,共鉴定得到2 343种蛋白质,其中差异表达蛋白质有284种(差异倍数≥2.0),初步构建了水牛卵巢黄体形成和退化的定量蛋白质表达谱。对其中的五种重要差异蛋白质(CD14、GTF2I、HSD17B1、LSM1和SERPINE1)进行qRT-PCR验证,结果有四种(CD14、HSD17B1、LSM1和SERPINE1)的qPCR结果与质谱结果相一致,仅GTF2I与质谱结果不一致,可能是由于GTF2I存在转录后调控。本研究首次从蛋白质水平对水牛卵巢排卵后的周期性变化的分子机制进行了探究,同时为其他家畜品种发情周期的研究提供了新的研究思路。     

质体醌重组的D1／D2／Cytb559复合物的荧光衰减动力学和光破坏作用

光系统Ⅱ反应中心Ｄ１／Ｄ２／Ｃｙｔｂ５５９ 在分离纯化过程中失去了电子受体ＱＡ 和ＱＢ,人工合成的质体醌可以与Ｄ１／Ｄ２／Ｃｙｔｂ５５９ 复合物发生重组。癸基质体醌（ＤＰＱ）与Ｄ１／Ｄ２／Ｃｙｔｂ５９９ 复合物的重组导致该复合物的荧光强度下降及发射光谱蓝移,同时两个与光化学活性相关的长寿命（２４ ｎｓ和７３ ｎｓ）荧光衰减组分占整个荧光的百分数下降,这些结果表明ＤＰＱ作为Ｐｈｅｏ－ 的电子受体,限制了Ｐ６８０＋ ·Ｐｈｅｏ－ 的电荷重组。ＤＰＱ 的加入对Ｄ１／Ｄ２／Ｃｙｔｂ５５９复合物中Ｃｈｌａ 分子的光破坏敏感性影响不大,但β－胡萝卜素在加入ＤＰＱ 之后可以被光照破坏,这个过程可能与β－胡萝卜素的生理功能相关。     

RORα高表达对二烯丙基二硫抑制人胃癌细胞增殖与迁移侵袭的影响

目的观察高表达RORα对二烯丙基二硫(DADS)抑制人胃癌MGC803细胞增殖、迁移与侵袭的影响。方法集落形成实验与流式细胞术检测细胞增殖与细胞周期;细胞划痕和Transwell实验分别检测细胞迁移与侵袭。RT-PCR与Western blot分别检测RORα、MMP-9和TIMP3 mRNA与蛋白表达水平。结果RT-PCR与Western blot检测显示,RORα高表达与DADS处理较对照组与空载体组RORαmRNA与蛋白表达明显上调,DADS+RORα高表达组上调更为显著(P<0.05)。与对照组和空载体组比较,RORα高表达与DADS处理组MMP-9表达下调,TIMP3表达上调,DADS+RORα高表达组改变最为显著。集落形成实验显示,RORα高表达与DADS处理组较对照组与空载体组的集落形成率明显降低。流式细胞术显示,与对照组和空载体组比较,RORα高表达与DADS处理组G2/M期细胞比率明显升高。细胞划痕和Transwell实验显示,RORα高表达与DADS处理组细胞迁移与侵袭能力明显降低。结论RORα高表达可通过上调TIMP3与下调MMP-9促进DADS阻滞MGC803细胞G2/M期和抑制增殖与迁移侵袭。     

Carbon uptake in Eurasian boreal forests dominates the high-latitude net ecosystem carbon budget

Arctic-boreal landscapes are experiencing profound warming, along with changes in ecosystem moisture status and disturbance from fire. This region is of global importance in terms of carbon feedbacks to climate, yet the sign (sink or source) and magnitude of the Arctic-boreal carbon budget within recent years remains highly uncertain. Here, we provide new estimates of recent (2003–2015) vegetation gross primary productivity (GPP), ecosystem respiration (R_eco), net ecosystem CO₂ exchange (NEE; R_eco − GPP), and terrestrial methane (CH₄) emissions for the Arctic-boreal zone using a satellite data-driven process-model for northern ecosystems (TCFM-Arctic), calibrated and evaluated using measurements from >60 tower eddy covariance (EC) sites. We used TCFM-Arctic to obtain daily 1-km² flux estimates and annual carbon budgets for the pan-Arctic-boreal region. Across the domain, the model indicated an overall average NEE sink of −850 Tg CO₂-C year⁻¹. Eurasian boreal zones, especially those in Siberia, contributed to a majority of the net sink. In contrast, the tundra biome was relatively carbon neutral (ranging from small sink to source). Regional CH₄ emissions from tundra and boreal wetlands (not accounting for aquatic CH₄) were estimated at 35 Tg CH₄-C year⁻¹. Accounting for additional emissions from open water aquatic bodies and from fire, using available estimates from the literature, reduced the total regional NEE sink by 21% and shifted many far northern tundra landscapes, and some boreal forests, to a net carbon source. This assessment, based on in situ observations and models, improves our understanding of the high-latitude carbon status and also indicates a continued need for integrated site-to-regional assessments to monitor the vulnerability of these ecosystems to climate change.     

酸角研究进展

从酸角的地理分布,生物生态学特性,类型划分,酸角的开发利用,酸角的栽培管理技术等方面,对酸角的研究进展进行了综述;探讨了酸角研究存在的问题及对策,以期为酸角的进一步研究提供参考。