Proteome analysis of hepatocellular carcinoma by two-dimensional difference gel electrophoresis: novel protein markers in hepatocellular carcinoma tissues

Hepatocellular carcinoma (HCC) is a highly malignant tumor, and chronic infection with hepatitis B virus is one of its major risk factors. To identify the proteins involved in HCC carcinogenesis, we used two-dimensional fluorescence DIGE to study the differentially expressed proteins in tumor and adjacent nontumor tissue samples. Samples from 12 hepatitis B virus-associated HCC patients were analyzed. A total of 61 spots were significantly up-regulated (ratio >/= 2, p 相似文献   

思茅松HDR基因全长cDNA克隆与序列分析

1-羟基-2-甲基-2-E-丁烯基-4-焦磷酸还原酶(HDR)是甲基-D-赤藓醇-4-磷酸(MEP)途径中的最后一个酶,在植物萜类生物合成中起主控作用。该研究根据思茅松(Pinus kesiya var.langbianensis)树皮转录组数据分析结果,首先获得了思茅松HDR基因片段,然后根据所获得的基因片段设计特异引物,提取受伤后的思茅松树皮的RNA,并运用RT-PCR和RACE技术从思茅松树皮中克隆得到完整的HDR基因(Pk HDR)。生物信息学分析表明:克隆获得的Pk HDR1基因c DNA全长序列为1 876 bp,含有1个1 464 bp的开放阅读框(ORF),编码487个氨基酸。同源性分析结果表明:思茅松HDR蛋白与赤松(Pinus densiflora)HDR蛋白的相似性高达99%。亚细胞定位及结构域分析结果表明:思茅松Pk HDR氨基酸序列中包含转运肽序列(A1-A61)及植物HDR蛋白多个保守的功能位点(A143,A234,A288,A371)。系统进化分析结果表明:Pk HDR蛋白与赤松HDR蛋白的亲缘关系最为接近。半定量PCR检测结果表明:树皮的创伤促进思茅松HDR基因的表达。该研究成功克隆获得HDR基因,并确定其与松脂代谢密切相关,为阐明思茅松松脂生物合成机制和分子育种提供了参考。     

Field Studies of the Wheat Stomata Resistance Influenced by Soil Water Content

Concurrent observations of soil water potential and leaf stomata diffusion resistance were made on two, plots of wheat grown at Datun Agro-ecological Experimental Station in Beijing under two different soil water conditions. These data were further complemented by weather and physiological observation. In this paper, we mainly analysed the influence of soil water potential on the status of wheat leaf stomatal resistance. The results indicate that: (1) there is a obvious influence of soil water potential on the status of wheat leaf stomata under normal conditions and (2) there is a different upper and lower epidermis stomata of wheat leaf respond to the soil water potential. The lower epidermis stomata are more sensitive to soil water potential than upper epidermis one. (3) There is a linear relationship between the ratio of lower and upper epidermis stomata resistance and soil water potential in root layer, according to this we can diagnose the degree of wheat water deficit.     

<Emphasis Type="Italic">Sphingomonas humi</Emphasis> sp. nov., isolated from soil

A Gram-negative, non-motile, non-spore-forming, small, orange, rod-shaped bacterium was isolated from soil in South Korea and characterized to determine its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequence examination revealed that strain PB323^T belongs to the family Sphingomonadaceae. The highest degree of sequence similarity was found with Sphingomonas kaistensis PB56^T (98.9%), followed by Sphingomonas astaxanthinifaciens TDMA-17^T (98.3%). Chemotaxonomic characteristics (the G+C content of the genomic DNA 69.0 mol%, Q-10 quinone system, C_18:1 ω7c/ω9t/ω12t, C_16:1 ω7c/C_15:0 iso 2OH, C_17:1 ω6c, and C16:0 as the major fatty acids) corroborated assignment of strain PB323^T to the genus Sphingomonas. Results of physiological and biochemical tests clearly demonstrate that strain PB323^T represents a distinct species and support its affiliation with the genus Sphingomonas. Based on these data, PB323^T (=KCTC 12341^T =JCM 16603T =KEMB 9004-003^T) should be classified as a type strain of a novel species, for which the name Sphingomonas humi sp. nov. is proposed.     

建鲤和异育银鲫摄食不同质量饲料时的氮收支和能量收支比较

实验探讨了建鲤和异育银鲫摄食低质和高质饲料时氮和能量的收支情况.低质饲料以豆粕为主要蛋白源,饲料蛋白含量为33.91%,高质饲料以鱼粉为主要蛋白源,饲料蛋白含量为45.59%.55d的生长结果显示,氮收支和能量收支受到饲料质量和鱼类种类的显著影响:摄食低质饲料时,建鲤的生长氮和生长能比例显著低于异育银鲫,排泄氮、排泄能和代谢能比例显著高于异育银鲫;摄食高质饲料时,两种鱼的氮收支和能量收支无显著差异;建鲤的氮收支和能量收支受饲料质量的显著影响,摄食低质饲料时,其生长氮和生长能比例均显著低于摄食高质饲料时,而排泄氮、粪能和代谢能比例均显著高于摄食高质饲料时;异育银鲫的氮收支、生长能和代谢能比例不受饲料质量的显著影响.结果表明,在低质饲料条件下,建鲤利用氮和能量的能力弱于异育银鲫,在高质饲料条件下,两种鱼没有显著差异.与异育银鲫相比,建鲤利用氮和能量的能力受饲料质量的影响更为显著.       

Depletion of invariant NKT cells reduces inflammation-induced preterm delivery in mice

This study sought to determine whether invariant NKT (iNKT) cells play an essential role in inflammation-induced preterm delivery. Preterm delivery and fetal death rates were determined in wild-type (WT) C57BL/6 mice and iNKT cell-deficient Jα18(-/-) mice injected i.p. with LPS. The percentages of decidual immune cells, including activated subsets, and costimulatory molecule expression were analyzed by flow cytometry. Th1 and Th2 cytokine production in the culture supernatants of decidual mononuclear cells was measured by ELISA. To some extent, Jα18(-/-) mice were resistant to LPS-induced preterm delivery. The proportions of decidual CD3(+) and CD49b(+) cells were slightly lower in Jα18(-/-) mice than in WT Jα18(+/+) mice, whereas almost no CD3(+)CD49b(+) cells could be found in Jα18-null mice. The percentages of activated decidual DCs, T cells, and NK cells were significantly lower in LPS-treated Jα18(-/-) mice than in WT mice. The CD40, CD80, and CD86 expression levels on decidual CD11c(+) cells from Jα18(-/-) mice were also significantly lower than in WT mice. Mean concentrations of Th1 cytokines IFN-γ and IL-12p70 in the culture supernatants of decidual mononuclear cells from LPS-treated Jα18(-/-) mice were apparently lower than those of LPS-induced WT mice. Additionally, the proportions of activated CD11c(+) cells, CD3(+) cells, and CD49b(+) cells in LPS-induced preterm delivery mice were strikingly higher in both WT and null mice when compared with the control PBS group and LPS-injected but normally delivered mice. Our results suggest that iNKT cells may play an essential role in inflammation-induced preterm birth.     

独叶草的不育雄蕊、花被片和可育雄蕊对传粉昆虫和传粉的影响

为确定独叶草（Kingdonia uniflora）不育雄蕊、花被片和可育雄蕊对传粉昆虫和传粉的影响,分别进行去除不育雄蕊（A组）,去除花被片（B组）,去除可育雄蕊（C组）和对照组（D组）处理。结果表明：昆虫访花频率分别为A组0．4次／h、B组0次／h、C组0．9次／h、D组2．2次／h;被授粉花朵百分比分别为A组29．09％、B组40．38％、C组70.91％、D组91．67％;被授粉心皮百分比分别为A组17．77％、B组20．94％、C组为40．58％、D组为75．27％。与对照组相比,处理后被授粉花朵百分比、被授粉心皮百分比下降幅度均为A组〉B组〉C组。结合昆虫访花频率与不育雄蕊蜜汁的分泌量呈正比,可确定不育雄蕊产生的蜜汁是吸引昆虫访花的最主要因素。花被片为昆虫取食提供停歇的平台,可育雄蕊与花被片产生的色差及可育雄蕊提供的花粉,是吸引昆虫的辅助因素。