EFFECTS OF HELPER PROTEINS ENCODED BY p19 AND orf1-orf2 GENES ON Cyt1Aa PROTEIN EXPRESSION IN ACRYSTALLIFEROUS STRAIN OF BACILLUS THURINGIENSIS

A series of plasmids were constructed to examine the effects of p19 and orf1‐orf2 genes from Bacillus thuringiensis on Cyt1Aa synthesis and inclusion formation. The plasmids expressed the cyt1Aa gene along with either p19 or orf1‐orf2, or each of them coordinatively with p20 in the acrystalliferous strain of B. thuringiensis subsp. israelensis 4Q7. No effect on the expression of Cyt1Aa protein was found when P19 or Orf1‐Orf2 co‐expressed with Cyt1Aa. However, when including p20 gene, the constructs with p19 or orf1‐orf2 gene produced lower yield of Cyt1Aa proteins than without p19 or orf1‐orf2 gene. Electron microscopy observation and bioassay showed that P19 and Orf1‐Orf2 have no influence on the crystal size and toxicity of Cyt1Aa protein. It is presumed that P19 and Orf1‐Orf2 might have negative effects on Cyt1Aa synthesis in B. thuringiensis.     

海甘蓝种子成熟过程中脂肪酸含量以及脱落酸和山梨醇的效应

海甘蓝种子在成熟过程中,棕榈酸、硬脂酸和亚麻酸的含量不断下降,而二十碳烯酸和芥酸的含量呈上升趋势。选用开花后２５～２７ｄ的海甘蓝幼胚分别在含不同浓度的ＡＢＡ或高渗透剂的培养基中培养１～３ｄ,发现其各种脂肪酸的变化趋势和种子自然成熟过程中脂肪酸的变化相似,说明ＡＢＡ或高渗透剂可能是种子成熟过程中各种脂肪酸合成和相互转化所需的条件。     

大麦G－带变动性以及与染色粒的一致性分析

以大麦为材料,进行了Ｇ－带带纹变动性分析以及Ｇ－带与减数分裂粗线期染色粒的比较。有丝分裂早中期至中期两个不同时期Ｇ－带带纹总数减少３６％,染色体组的绝对长度缩短２５％,带数减少的幅度大于染色体长度缩短的幅度。染色体组中每条染色体之间带纹减少的比例不尽相同,变幅在０．２９－０．５０之间。不同染色体绝对长度减少的幅度在０．２７－３．７０μ之间。从早中期至中期,每单位染色体绝对长度带数具相对减少的趋势。选择第６染色体进行的比较显示,减数分裂的染色粒与有丝分裂的Ｇ－带带纹之间具有很好的对应关系,Ｇ－带带纹和染色粒间的数目、位置和着色程度上都具一致性。对Ｇ－带性质和植物中Ｇ－带的应用等问题进行了讨论。     

An efficient synthesis of methyl tetra-o-hexyl gentiooctaoside, an octaosyl analogue of ANP receptor antagonist HS-142-1

Methyl

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, a 3-O hexyl analogue of the octaosyl component of fungal lipooligosaccharide HS-142-1, was stereo- and regioselectively synthesized as a potent antagonist for the tetrameric atrial natriuretic peptide (ANP) receptors.     

两阶抽样中比率型估计量的平均精度

本文给出了两阶抽样中总体均值的比率型估计量的平均精度,它当样本容量充分大时主项不劣于无偏估计量的平均精度．     

特种油料植物的种质资源研究

本文对特种油料植物的种质资源研究进行综述,这些植物包括含长链脂肪酸的Crambe,Limnanthes,Lunaria,短链脂肪酸的Cuphea,Lauraceae,羟基脂肪酸的Lesquerella,环氧脂肪酸的Vernonia,Stokesia,以及液体蜡酯的simmondsia等科、属的植物。     

生物制品检定动态管理系统的开发和应用

掌握生物制品的检定动态是质量管理的重要内容,由于检定周期随着制品种类、批数及检定条件的变化而变化,以往靠手工方式查阅繁杂的检定记录,难以随时快速、全面了解当时的检定状况。检定动态管理软件的开发可应用计算机自动跟踪显示检定进度和检定状态,及时反映制品质量和检定条件变化,明显提高了生产和质量管理部门的工作效率。     

Spatial expression dynamics of Men-9 delineate the third floral whorl in male and female flowers of dioecious Silene latifolia

Sex determination in Silene latifolia is controlled by heteromorphic sex chromosomes. Female flowers have five fused carpels and ten arrested stamen primordia. The male-determining Y chromosome overrides female development to suppress carpel formation and promote stamen development. The isolation and characterization of two S. latifoliaM ale en hanced cDNAs, Men-9a and Men-9b, which probably represent different alleles of a novel gene are reported here. Men-9a and Men-9b share 91.8% coding sequence nucleotide identity, yet only 85.4% amino acid identity. The Men-9 cDNAs are related to the previously reported MROS3 cDNA from S. latifolia. However, MROS3 is not present in the S. latifolia population used in these studies and the expression dynamics of Men-9a and Men-9b contrast dramatically with those reported for MROS3. Men-9 cDNAs are expressed primarily in anthers of young male flowers, with highest expression in 1–2 mm buds. Men-9 expression is also observed at a low level in female flowers. In situ hybridization analysis reveals two phases of Men-9 expression. The first phase is during a common stage of early stamen development in male and female flowers prior to stamen arrest in female flowers. The second phase of Men-9 expression is maximal in the epidermis and endothecium of Y chromosome- and Ustilago violacea-induced stamens; expression in male and female flowers extends to the epidermis of the staminal nectaries with strict boundaries at the second and fourth whorls. Men-9 gene expression therefore delineates the boundaries of the third floral whorl in S. latifolia flowers.     

纤蚤属一新种及一新纪录（蚤目：多毛蚤科）

本文报道纤蚤属１９１２ １新种及１新纪录：蔡氏纤蚤和五侧纤蚤似邻近亚种。