FIBONACCI级数与菊花结构规律的研究

本文论证了菲波纳斯（Fibonacci）级数与菊花花冠结构的关系,发现了花冠的通用数学模式：花冠（数目）＝（5×倍数）十F_n（F_(n－1)＋F_(n-2)）     

武陵山地区鱼类寄生杆咽属线虫两新种

本文报道在武陵山地区的白甲鱼［（Ｖａｒｉｃｏｒｋｉｎｕｓ（Ｏｎｙｃｈｏｓｔｏｍａ）ｓｉｍｕｓ（ＳａｕｖａｇｅｅｔＤａｂｒｙ）］和沪溪直口峻（ＲｅｃｔｏｒｉｓｌｕｘｉｅｎｓｉｓＷｕｅｔＹａｏ）中发现的两种杆咽属（Ｒｈａｂｄｏｃｈｏｎａ）线虫新种,定名为白甲鱼杆咽线虫,新种（ＲｈａｂｄＯｃｈｏｎａｏｎｙｒｋｏｓｔｏｍｉｓｐ．ｎｏｖ．）和短咽杆咽线虫,新种（ＲｈａｂｄＯｃｈｏｎａｂｒｅｖｉｃｈｏｎａｓｐ．ｎｏｖ．）,并对两新种进行了测量和详细的描述。     

Systemic amyloidosis in transgenic mice carrying the human mutant transthyretin (Met 30) gene

To analyze the pathologic processes of amyloid deposition in type I familial amyloidotic polyneuropathy (FAP), mice were made transgenic by introducing the human mutant transthyretin (TTR) gene(MT-hMet 30). An inbred strain of mouse, C57 BL/6, was chosen. Transgenic mice were killed using ether anesthesia at 3-mo intervals up to 24 mo after birth. In these transgenic mice, amyloid deposition started in the gastrointestinal tract, cardiovascular system, and kidneys and extended to various other organs and tissues with advancing age. The pattern of amyloid deposition was similar to that observed in human autopsy cases of FAP, except for its absence in the choroid plexus and in the peripheral and autonomic nervous systems. We extracted the amyloid fibrils from kidneys of these mice with a human mutant TTR gene and analyzed them immunochemically and electronmicroscopically. Deposited amyloid was shown to be composed of human mutant TTR and mouse serum amyloid P component. Amyloid fibril from transgenic mice was morphologically and immunohistochemically similar to that of human FAP. The most striking pathologic feature of the transgenic mice was the absence of amyloid deposition in the peripheral and autonomic nervous tissues. Thus, other intrinsic factors may be involved in amyloid deposition in the nervous tissues of human FAP.     

Regulation of vasoactive intestinal peptide expression in sympathetic neurons in culture and after axotomy: The role of cholinergic differentiation factor/leukemia inhibitory factor

Vasoactive intestinal peptide (VIP) expression increases in sympathetic neurons when they are grown in dissociated cell or explant cultures and when they are axotomized in vivo. In dissociated cell culture, the magnitude of the VIP increase was reduced when nonneuronal cells were removed and medium conditioned by ganglionic nonneuronal cells increased VIP in neuron-enriched cultures. Antiserum Against cholinergic differentiation factor (also leukemia inhibitory factor; CDF/LIF), but not against ciliary neurotrophic factor, immunoprecipitated this activity. Medium conditioned by sympathetic ganglion explants also contained a VIP-stimulatory molecule that was immunoprecipitated by CDF/LIF antiserum, and CDF/LIF antiserum partially blocked VIP induction in explants. CDF/LIF mRNA was increased in dissociated cell cultures, in ganglion explants and in vivo after axotomy. Our results suggest that CDF/LIF released from ganglionic nonneuronal cells plays an important role in regulating VIP after axotomy. 1994 John Wiley & Sons, Inc.     

EFFECTS OF HELPER PROTEINS ENCODED BY p19 AND orf1-orf2 GENES ON Cyt1Aa PROTEIN EXPRESSION IN ACRYSTALLIFEROUS STRAIN OF BACILLUS THURINGIENSIS

A series of plasmids were constructed to examine the effects of p19 and orf1‐orf2 genes from Bacillus thuringiensis on Cyt1Aa synthesis and inclusion formation. The plasmids expressed the cyt1Aa gene along with either p19 or orf1‐orf2, or each of them coordinatively with p20 in the acrystalliferous strain of B. thuringiensis subsp. israelensis 4Q7. No effect on the expression of Cyt1Aa protein was found when P19 or Orf1‐Orf2 co‐expressed with Cyt1Aa. However, when including p20 gene, the constructs with p19 or orf1‐orf2 gene produced lower yield of Cyt1Aa proteins than without p19 or orf1‐orf2 gene. Electron microscopy observation and bioassay showed that P19 and Orf1‐Orf2 have no influence on the crystal size and toxicity of Cyt1Aa protein. It is presumed that P19 and Orf1‐Orf2 might have negative effects on Cyt1Aa synthesis in B. thuringiensis.     

大麦G－带变动性以及与染色粒的一致性分析

以大麦为材料,进行了Ｇ－带带纹变动性分析以及Ｇ－带与减数分裂粗线期染色粒的比较。有丝分裂早中期至中期两个不同时期Ｇ－带带纹总数减少３６％,染色体组的绝对长度缩短２５％,带数减少的幅度大于染色体长度缩短的幅度。染色体组中每条染色体之间带纹减少的比例不尽相同,变幅在０．２９－０．５０之间。不同染色体绝对长度减少的幅度在０．２７－３．７０μ之间。从早中期至中期,每单位染色体绝对长度带数具相对减少的趋势。选择第６染色体进行的比较显示,减数分裂的染色粒与有丝分裂的Ｇ－带带纹之间具有很好的对应关系,Ｇ－带带纹和染色粒间的数目、位置和着色程度上都具一致性。对Ｇ－带性质和植物中Ｇ－带的应用等问题进行了讨论。     

An efficient synthesis of methyl tetra-o-hexyl gentiooctaoside, an octaosyl analogue of ANP receptor antagonist HS-142-1

Methyl

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, a 3-O hexyl analogue of the octaosyl component of fungal lipooligosaccharide HS-142-1, was stereo- and regioselectively synthesized as a potent antagonist for the tetrameric atrial natriuretic peptide (ANP) receptors.     

Atypical metachromatic leukodystrophy?

Summary A 3/12-year-old slightly retarded boy with marked deficiency of arylsulfatase A (ASA) activity in leucocytes and fibroblasts and almost no cerebroside sulfatase (CS) activity in fibroblasts was tested with the sulfatide-loading test. On this test his fibroblasts showed impaired degradation. A pathological excretion of sulfatides was seen in his urine. Nerve conduction velocity, visual evoked potential, auditory brain stem evoked response, and somatosensory evoked potential were all normal.His father and older brother had similarly low levels of ASA in leucocytes and fibroblasts and 1.7–2% residual CS activity in fibroblasts. Although both were clinically normal, their fibroblasts accumulated increased amounts of sulfatides when challenged in the sulfatide-loading test.In this family, this test thus will be of no value in prenatal diagnosis to discriminate among low ASA fetuses with pseudoarylsulfatase A deficiency and fetuses with this unusual ASA deficiency variant.     

中国种子植物区系定量化研究——Ⅰ区域性种子植物区系研究实用程序工作原理(QX—系列程序)

本文根据吴征镒教授对中国种子植物属分市区类型研究结果,研制出定量化研究区域性种子植物区系的电子计算机程序,应用本程序可完成某区系的分布区类型统计分析,科属组成分析和与其它地区以共有属关系构建的相似性系数的谁知盘中计算,同时可大量节省研究人员的劳动强度和时间,对提高研究水平有一定的效果。