湖北省革螨初步名录

根据文献报告及调查所得,湖北省已知革螨 Gamasid mite计有28属59种.文内对种名、宿主及分布予以详细记载     

中国东北地区基于环志监测的田鹀迁徙趋势和数量动态北大核心CSCD

许多长距离迁徙的雀形目鸟类的种群数量正在持续下降,田鹀(Emberiza rustica)种群数量下降趋势更为突出。通过对田鹀种群数量长期监测和迁徙动态分析,可为此物种保护提供科学依据。从2001年开始,陆续在黑龙江省高峰、青峰、帽儿山、新青和大沾河,吉林省珲春和吉林市,辽宁省的辽宁鸟类研究中心(大连)和旅顺老铁山,以及内蒙古乌尔其汗鸟类栖息的临水林缘处布网环志。截至2018年,累计环志田鹀184181只,其中春季88571只,秋季95610只;各年度环志数量波动较大,总体呈现急速下降趋势。幼鸟的越冬损失率高达41.3%。田鹀106只次的回收信息表明,自然条件下田鹀寿命可达11年以上;日迁飞距离最快可达到300 km,飞行速度可达30 km/h。中国东北地区是田鹀等鸟类的重要迁徙途经地;田鹀的迁徙路线相对稳定,在瑞典北部繁殖的种群经中国东北地区迁徙到天津以南越冬。通过环志发现,近些年田鹀种群数量急速下降。通过比对,发现中国东北地区田鹀的环志数量变化趋势与瑞典的田鹀环志数量变化趋势相似;相对于环志数量最多的年份,环志数量下降95%以上,值得关注。栖息地破碎化、非法猎捕等是影响田鹀生存的主要受威胁因素。建议依据田鹀等鸟类生物学习性,加强鸟类栖息地的保护,坚持长期标准化的鸟类环志监测,进一步探索鸟类迁徙规律,以助于鸟类种群的恢复。     

Effect of daily salinity fluctuation on the intraspecific interactions of a euhalophyte (Suaeda salsa) along a salinity gradient

每日盐度波动对真盐生植物盐地碱蓬种内相互作用沿盐度梯度的影响 土壤盐度的异质性是河口潮间带的一个突出的环境特征,影响植物的生长和盐沼中生物相互作用的转变。本研究旨在探究盐度梯度和盐度波动对一种真盐生植物的种内相互作用的交互影响。     

A metabolism-relevant signature as a predictor for prognosis and therapeutic response in pancreatic cancer

Although several altered metabolic genes have been identified to be involved in the tumorigenesis and advance of pancreatic cancer (PC), their prognostic values remained unclear. The purpose of this study was to explore new targets and establish a metabolic signature to predict prognosis and chemotherapy response for optimal individualized treatment. The expression data of PC patients from two independent cohorts and metabolism-related genes from KEGG were utilized and analyzed for the establishment of the signature via lasso regression. Then, the differentially expressed candidate genes were further confirmed via online data mining platform and qRT-PCR of clinical specimens. Then, the analyses of gene set enrichment, mutation, and chemotherapeutic response were performed via R package. As results showed, 109 differentially expressed metabolic genes were screened out in PC. Then a metabolism-related five-gene signature comprising B3GNT3, BCAT1, KYNU, LDHA, and TYMS was constructed and showed excellent ability for predicting survival. A novel nomogram coordinating the metabolic signature and other independent prognostic parameters was developed and showed better predictive power in predicting survival. In addition, this metabolic signature was significantly involved in the activation of multiple oncological pathways and regulation of the tumor immune microenvironment. The patients with high risk scores had higher tumor mutation burdens and were prone to be more sensitive to chemotherapy. In summary, our work identified a new metabolic signature and established a superior prognostic nomogram which may supply more indications to explore novel strategies for diagnosis and treatment.     

Identification and characterization of the aspartate chemosensory receptor of Campylobacter jejuni

Campylobacter jejuni is a highly motile bacterium that responds via chemotaxis to environmental stimuli to migrate towards favourable conditions. Previous in silico analysis of the C. jejuni strain NCTC11168 genome sequence identified 10 open reading frames, tlp1‐10, that encode putative chemosensory receptors. We describe the characterization of the role and specificity of the Tlp1 chemoreceptor (Cj1506c). In vitro and in vivo models were used to determine if Tlp1 had a role in host colonization. The tlp1^‐ isogenic mutant was more adherent in cell culture, however, showed reduced colonization ability in chickens. Specific interactions between the purified sensory domain of Tlp1 and l ‐aspartate were identified using an amino acid array and saturation transfer difference nuclear magnetic resonance spectroscopy. Chemotaxis assays showed differences between migration of wild‐type C. jejuni cells and that of a tlp1^‐ isogenic mutant, specifically towards aspartate. Furthermore, using yeast two‐hybrid and three‐hybrid systems for analysis of protein–protein interactions, the cytoplasmic signalling domain of Tlp1 was found to preferentially interact with CheV, rather than the CheW homologue of the chemotaxis signalling pathway; this interaction was confirmed using immune precipitation assays. This is the first identification of an aspartate receptor in bacteria other than Escherichia coli and Salmonella enterica serovar Typhimurium.     

Effects of clove oil,essential oil of Lippia alba and 2‐phe anaesthesia on juvenile meagre,Argyrosomus regius (Asso, 1801)

The objectives of this experiment were to (i) determine the efficacy of essential oils of clove (CO) and Lippia alba (EOLA) to induce deep anaesthesia in juvenile specimens (49.0 ± 6.2 g body mass, 16.6 ± 0.8 cm; n = 8 per treatment) of meagre (Argyrosomus regius); and (ii) study the feasibility of these substances, together with 2‐phenoxyethanol (2‐PHE), as potential sedatives [low concentration: (i) EOLA: 12 mg L^?1; (ii) CO: 1 mg L^?1; and (iii) 2‐PHE: 33 mg·L ^?1; n = 8 per treatment] for live fish transport of this species. All test were performed at a constant temperature (18°C). Thus, the main primary stress indicator (plasma cortisol) and secondary factors (plasma metabolites) were evaluated. In addition, growth hormone (GH) mRNA expression was also evaluated in the pituitary gland. The results indicated that EOLA is considered to be effective for deep anaesthesia when the concentration is close to 160 mg L^?1, while CO produces the same effect when lower concentrations are added (40–50 mg L^?1). Regarding sedative concentrations, a significant ~3‐fold increase in plasma cortisol levels was detected in the EOLA group when compared to control specimens. In addition, glucose levels were not reduced and significantly increased (~1.6‐fold) for 2‐PHE in relation to the control fish. None of the anaesthetics promoted a significant difference for GH expression with respect to the control group, but a significant ~2‐fold increase for 2‐PHE treatment with respect to the EOLA exposition was found in this gene expression. Results show that none of the anaesthetics analysed, at least in the ranges of concentrations used in this study (EOLA 12 mg L^?1, CO 1 mg L^?1, 2‐PHE 33 mg L^?1), are recommended for live fish transport, as shown by the absence of inhibition on the stress parameters assessed.     

Changes in photosynthetic capacity and antioxidant enzymatic systems in micropropagated <Emphasis Type="Italic">Zingiber officinale</Emphasis> plantlets during their acclimation

Ginger (Zingiber officinale Rosc.) plantlets were propagated in vitro and acclimated under different photosynthetic photon flux densities (60 and 250 μmol m⁻² s⁻¹ = LI and HI, respectively). Increases in chlorophyll (Chl) content and Chl a/b ratio were found under both irradiances. In vitro plantlets (day 0) exhibited a low photosynthesis, but chloroplasts from in vitro leaves contained well developed grana and osmiophillic globules. Photoinhibition in leaves formed in vitro was characterized by decrease of photochemical efficiency and quantum efficiency of photosystem 2 photochemistry in HI treatment during acclimation. The new leaves formed during acclimation in both treatments showed a higher photosynthetic capacity than the leaves formed in vitro. Also activities of antioxidant enzymes of micropropagated ginger plantlets changed during acclimation.     

热带季节雨林林窗边缘不同热力作用面热力效应的季节变化特征

通过对热带季节雨林雾凉季和湿热季昼间林窗区域不同热力作用面的热力效应初步分析,指出在西双版纳,不论是雾凉季还是湿热季,热带季节雨林林窗边缘壁面均具有不可忽视的热力作用,且由于受林缘树木的影响,热力效应较强的东侧,北侧林缘壁面最大区域出现位置高于次生林林窗,而强度小于次生林林窗,显示了林窗边缘壁面的热力效应除与太阳高度角,太阳辐射的时间长短和强度有关之外,林窗边缘树木高度也是不可忽视的因子,其结果可为进一步研究林窗小气候形成机制提供研究基础,为研究林窗更新及生物多样性问题提供科学参考。