全文获取类型
收费全文 | 51325篇 |
免费 | 17496篇 |
国内免费 | 3290篇 |
出版年
2024年 | 102篇 |
2023年 | 404篇 |
2022年 | 878篇 |
2021年 | 1646篇 |
2020年 | 2953篇 |
2019年 | 4673篇 |
2018年 | 4610篇 |
2017年 | 4739篇 |
2016年 | 5028篇 |
2015年 | 5463篇 |
2014年 | 5451篇 |
2013年 | 6024篇 |
2012年 | 4220篇 |
2011年 | 3695篇 |
2010年 | 4446篇 |
2009年 | 3044篇 |
2008年 | 2265篇 |
2007年 | 1711篇 |
2006年 | 1517篇 |
2005年 | 1446篇 |
2004年 | 1328篇 |
2003年 | 1306篇 |
2002年 | 1222篇 |
2001年 | 803篇 |
2000年 | 649篇 |
1999年 | 515篇 |
1998年 | 265篇 |
1997年 | 223篇 |
1996年 | 196篇 |
1995年 | 129篇 |
1994年 | 183篇 |
1993年 | 99篇 |
1992年 | 109篇 |
1991年 | 100篇 |
1990年 | 87篇 |
1989年 | 68篇 |
1988年 | 64篇 |
1987年 | 54篇 |
1986年 | 54篇 |
1985年 | 55篇 |
1984年 | 32篇 |
1983年 | 36篇 |
1982年 | 38篇 |
1981年 | 19篇 |
1980年 | 13篇 |
1979年 | 25篇 |
1978年 | 14篇 |
1976年 | 21篇 |
1975年 | 14篇 |
1973年 | 10篇 |
排序方式: 共有10000条查询结果,搜索用时 265 毫秒
971.
Three‐dimensional reconstruction and comparison of vacuolar membranes in response to viral infection
Xueting Wang Juncai Ma Xuejiao Jin Ning Yue Peng Gao Keith Ka Ki Mai Xian‐Bing Wang Dawei Li Byung‐Ho Kang Yongliang Zhang 《植物学报(英文版)》2021,63(2):353-364
The vacuole is a unique plant organelle that plays an important role in maintaining cellular homeostasis under various environmental stress conditions. However, the effects of biotic stress on vacuole structure has not been examined using three‐dimensional (3D) visualization. Here, we performed 3D electron tomography to compare the ultrastructural changes in the vacuole during infection with different viruses. The 3D models revealed that vacuoles are remodeled in cells infected with cucumber mosaic virus (CMV) or tobacco necrosis virus A Chinese isolate (TNV‐AC), resulting in the formation of spherules at the periphery of the vacuole. These spherules contain neck‐like channels that connect their interior with the cytosol. Confocal microscopy of CMV replication proteins 1a and 2a and TNV‐AC auxiliary replication protein p23 showed that all of these proteins localize to the tonoplast. Electron microscopy revealed that the expression of these replication proteins alone is sufficient to induce spherule formation on the tonoplast, suggesting that these proteins play prominent roles in inducing vacuolar membrane remodeling. This is the first report of the 3D structures of viral replication factories built on the tonoplasts. These findings contribute to our understanding of vacuole biogenesis under normal conditions and during assembly of plant (+) RNA virus replication complexes. 相似文献
972.
In eukaryotes, autophagy helps maintain cellular homeostasis by degrading and recycling cytoplasmic materials via a tightly regulated pathway.Over the past few decades, significant progress has been made towards understanding the physiological functions and molecular regulation of autophagy in plant cells. Increasing evidence indicates that autophagy is essential for plant responses to several developmental and environmental cues, functioning in diverse processes such as senescence, male fertility, root meristem maintenance, responses to nutrient starvation,and biotic and abiotic stress. Recent studies have demonstrated that, similar to nonplant systems,the modulation of core proteins in the plant autophagy machinery by posttranslational modifications such as phosphorylation, ubiquitination,lipidation, S-sulfhydration, S-nitrosylation, and acetylation is widely involved in the initiation and progression of autophagy. Here, we provide an overview of the physiological roles and posttranslational regulation of autophagy in plants. 相似文献
973.
Ana Marcia Escocard de Azevedo Manhaes Fausto Andres Ortiz-Morea Ping He Libo Shan 《植物学报(英文版)》2021,63(1):79-101
As sessile organisms, plants are exposed to pathogen invasions and environmental fluctuations. To overcome the challenges of their surroundings, plants acquire the potential to sense endogenous and exogenous cues, resulting in their adaptability. Hence, plants have evolved a large collection of plasma membrane-resident receptors, including RECEPTOR-LIKE KINASEs(RLKs) and RECEPTOR-LIKE PROTEINs(RLPs) to perceive those signals and regulate plant growth,development, and immunity. The ability of RLKs and RLPs to recognize distinct ligands relies on diverse categories of extracellular domains evolved. Co-regulatory receptors are often required to associate with RLKs and RLPs to facilitate cellular signal transduction. RECEPTOR-LIKE CYTOPLASMIC KINASEs(RLCKs) also associate with the complex, bifurcating the signal to key signaling hubs, such as MITOGEN-ACTIVATED PROTEIN KINASE(MAPK) cascades, to regulate diverse biological processes. Here, we discuss recent knowledge advances in understanding the roles of RLKs and RLPs in plant growth, development, and immunity, and their connection with co-regulatory receptors, leading to activation of diverse intracellular signaling pathways. 相似文献
974.
975.
976.
977.
Jie Wang Long‐Jiang Yu Wenda Wang Qiujing Yan Tingyun Kuang Xiaochun Qin Jian‐Ren Shen 《植物学报(英文版)》2021,63(7):1367-1381
Photosystem I (PSI) is one of the two photosystems in photosynthesis, and performs a series of electron transfer reactions leading to the reduction of ferredoxin. In higher plants, PSI is surrounded by four light-harvesting complex I (LHCI) subunits, which harvest and transfer energy efficiently to the PSI core. The crystal structure of PSI-LHCI supercomplex has been analyzed up to 2.6 Å resolution, providing much information on the arrangement of proteins and cofactors in this complicated supercomplex. Here we have optimized crystallization conditions, and analyzed the crystal structure of PSI-LHCI at 2.4 Å resolution. Our structure showed some shift of the LHCI, especially the Lhca4 subunit, away from the PSI core, suggesting the indirect connection and inefficiency of energy transfer from this Lhca subunit to the PSI core. We identified five new lipids in the structure, most of them are located in the gap region between the Lhca subunits and the PSI core. These lipid molecules may play important roles in binding of the Lhca subunits to the core, as well as in the assembly of the supercomplex. The present results thus provide novel information for the elucidation of the mechanisms for the light-energy harvesting, transfer and assembly of this supercomplex. 相似文献
978.
Potassium and phosphorus transport and signaling in plants 总被引:2,自引:0,他引:2
Nitrogen(N), potassium(K), and phosphorus(P) are essential macronutrients for plant growth and development, and their availability affects crop yield. Compared with N, the relatively low availability of K and P in soils limits crop production and thus threatens food security and agricultural sustainability. Improvement of plant nutrient utilization efficiency provides a potential route to overcome the effects of K and P deficiencies. Investigation of the molecular mechanisms underlying how plants sense, absorb, transport, and use K and P is an important prerequisite to improve crop nutrient utilization efficiency. In this review, we summarize current understanding of K and P transport and signaling in plants, mainly taking Arabidopsis thaliana and rice(Oryza sativa) as examples. We also discuss the mechanisms coordinating transport of N and K, as well as P and N. 相似文献
979.
980.
目的:探讨血清核心蛋白多糖(DCN)、神经调节蛋白-1(NRG-1)、巨噬细胞迁移抑制因子(MIF)水平与首发未服药精神分裂症患者临床症状和认知功能的相关性。方法:选择2018年1月~2020年11月期间长江大学附属第一医院收治的首发未服药精神分裂症患者80例作为精神分裂症组,同期于长江大学附属第一医院进行体检的健康志愿者80例作为对照组。应用阳性和阴性症状量表(PANSS)评估患者精神病理症状,应用MATRICS共识认知成套测验(MCCB)评估所有受试者认知功能。根据PANSS评分将精神分裂症组分为PANSS评分高分组和低分组,比较两组血清DCN、NRG-1、MIF水平,并分析以上指标水平与PANSS总分、MCCB各项评分的相关性。结果:精神分裂症组患者PANSS总分为(77.18±13.57)分。精神分裂症组MCCB各项评分均低于对照组(P<0.05)。精神分裂症组血清DCN、NRG-1水平低于对照组,MIF水平高于对照组(P<0.05)。PANSS高分组血清DCN、NRG-1水平低于PANSS低分组,MIF水平高于PANSS低分组(P<0.05)。Pearson相关性分析显示,首发未服药精神分裂症患者血清DCN、NRG-1水平与PANSS总分呈负相关,与MCCB各项评分呈正相关,MIF水平与PANSS总分呈正相关,与MCCB各项评分呈负相关(均P<0.05)。结论:首发未服药精神分裂症患者血清DCN、NRG-1、MIF水平异常,且以上指标水平与患者临床症状和认知功能受损有一定联系,提示检测以上指标水平可能为该病患者认知功能及临床症状的评估提供参考。 相似文献