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Serebryakova Larisa Studneva Irina Timoshin Alexander Veselova Oksana Pal’keeva Marina Ovchinnikov Michael Az’muko Andrey Molokoedov Alexander Sidorova Maria Pisarenko Oleg 《International journal of peptide research and therapeutics》2021,27(3):2039-2048
International Journal of Peptide Research and Therapeutics - Natural and chemically modified N-terminal galanin fragments (WTLNSAGYLLGPHA-OH (G1) and WTLNSAGYLLGPβAH-OH (G2), respectively)... 相似文献
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Serebryakova Larisa Studneva Irina Timoshin Alexander Veselova Oksana Pal’keeva Marina Ovchinnikov Michael Az’muko Andrey Molokoedov Alexander Sidorova Maria Pisarenko Oleg 《International journal of peptide research and therapeutics》2021,27(3):2049-2049
International Journal of Peptide Research and Therapeutics - A correction to this paper has been published: https://doi.org/10.1007/s10989-021-10233-9 相似文献
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Previous studies showed that cell suspensions of unicellular nondiazotrophic cyanobacterium G. alpicola grown under nitrate-limiting conditions intensively produces H2 via fermentation of endogenous glycogen with hydrogen yield more then 90% of theoretical maximum (3.8 mol H2 per mol glucose). H2 production is realized by a Hox hydrogenase on the stages of NAD(P)H generation. Exploiting this property, the two-stage cyclic system for sustained hydrogen production was developed using a photobioreactor (PhBR) with G. alpicola immobilized on glass fiber TR-0.3. Immobilization of the cells on the matrix occurred during growth directly in PhBR operated in continuous mode; the density of culture immobilized achieved 37 g Chl alpha cm(-2). The first stage of the cycle was the photosynthetic incubations of G. alpicola in the flow of the culture medium, which contained limiting concentrations of nitrate for efficient glycogen accumulation and activation of hydrogenase. The second stage was the fermentation of glycogen, with H2 production realized in darkness with continuous Ar sparging and without medium flow. Standardization of optimal parameters for both stages provided a stable cyclic regime of the system: photosynthesis (24 hours)-fermentation (24 hours). The total amount of H2 evolved in one cycle was 957.6 mL L(-1)(matrix), and the overage rate of H2 production during the cycle (48 hours) was about 20 mL h(-1) L(-1)(matrix). Ten consequent cycles was carried out in this regime with reproducible H2 production, although PhBR with the same sample of immobilized culture was operated over a period of more then three months. 相似文献
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Inhibition of the GTPase dynamin or actin depolymerisation initiates outward plasma membrane tubulation/vesiculation (cytoneme formation) in neutrophils
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Yu. P. Kozmin A. V. Manoilov M. V. Serebryakova O. A. Mirgorodskaya 《Russian Journal of Bioorganic Chemistry》2011,37(6):719-731
A method for direct introduction of 18O isotopes into carboxyl groups of peptides and proteins via the exchange with H2
18O in the presence of TFA is described. The isotope label is sufficiently stable in a wide pH range. Since the compounds labeled
by this method retain their physicochemical characteristics, they can be used as an internal standard in quantitative assay
of authentic compounds in the analyzed objects by means of mass spectrometry. This method is applicable to quantitative analysis
of peptides and proteins in biological environments, as well as for quantitative kinetic studies of metabolism and enzyme
activity. The quantitative analysis of polypeptides and proteins is combined with trypsinolysis. When necessary, the isotope
label can be simultaneously introduced into all peptides and proteins in a control biosample, making it applicable as a standard
for comparative analysis of experimental biosamples. 相似文献
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I. A. Demina M. V. Serebryakova V. G. Ladygina M. A. Rogova V. G. Zgoda D. A. Korzhenevskyi V. M. Govorun 《Biochemistry. Biokhimii?a》2009,74(2):165-174
Using modern proteomic assays, we have identified the products of gene expression and posttranslational modifications of proteins of the bacterium Mycoplasma gallisepticum S6. Combinations of different technologies of protein separation by electrophoresis and mass-spectrometric analysis gave us a total of 446 proteins, i.e. 61% of the annotated proteins of this microorganism. The Pro-Q Diamond and Pro-Q Emerald dye technology was used for fluorescent detection of ten phosphoproteins and two glycoproteins. The acylation of proteins was studied by electrophoresis after in vivo labeling with different 14C-labeled fatty acids, followed by autoradiography. Sixteen acylated proteins were identified, with a quarter of them involved in plasma membrane construction and another quarter involved in cell energy metabolism. 相似文献
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Anna Y. Golovina Petr V. Sergiev Andrey V. Golovin Marina V. Serebryakova Irina Demina Vadim M. Govorun Olga A. Dontsova 《RNA (New York, N.Y.)》2009,15(6):1134-1141
Transfer RNA is highly modified. Nucleotide 37 of the anticodon loop is represented by various modified nucleotides. In Escherichia coli, the valine-specific tRNA (cmo5UAC) contains a unique modification, N6-methyladenosine, at position 37; however, the enzyme responsible for this modification is unknown. Here we demonstrate that the yfiC gene of E. coli encodes an enzyme responsible for the methylation of A37 in tRNA1Val. Inactivation of yfiC gene abolishes m6A formation in tRNA1Val, while expression of the yfiC gene from a plasmid restores the modification. Additionally, unmodified tRNA1Val can be methylated by recombinant YfiC protein in vitro. Although the methylation of m6A in tRNA1Val by YfiC has little influence on the cell growth under standard conditions, the yfiC gene confers a growth advantage under conditions of osmotic and oxidative stress. 相似文献