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111.
112.
Kristof De Prijck Nele De Smet Kris Honraet Steven Christiaen Tom Coenye Etienne Schacht Hans J. Nelis 《Mycopathologia》2010,169(3):167-174
Unlike various disinfectants, antifungals have not been commonly incorporated so far in medical devices, such as catheters
or prostheses, to prevent biofilm formation by Candida spp. In the present study, five antimycotics were added to polydimethyl siloxane (PDMS) disks via admixture (nystatin) or
impregnation (trimethylsilyl-nystatin (TMS-nystatin), miconazole, tea tree oil (TTO), zinc pyrithione). Nystatin-medicated
PDMS disks exhibited a concentration-dependent inhibitory effect on biofilm formation in a microtiter plate (MTP) but not
in a Modified Robbins Device (MRD). This observation, together with HPLC data and agar diffusion tests, indicates that a small
fraction of free nystatin is released, which kills Candida albicans cells in the limited volume of a MTP well. In contrast, biofilm inhibition amounted to more than one log unit in the MRD
on disks impregnated with miconazole, TTO, and zinc pyrithione. It is hypothesized that the reduction in biofilm formation
by these compounds in a flow system occurs through a contact-dependent effect. 相似文献
113.
Michiel Etienne Janssens Dirk Geysen Katleen Broos Ine De Goeyse Johan Robbens Filip Van Petegem Jean-Pierre Timmermans Yves Guisez 《Amino acids》2010,38(5):1617-1626
The hepatitis B core (HBc) protein has been used successfully in numerous experiments as a carrier for heterologous peptides.
Folding and capsid formation of the chimeric proteins is not always achieved easily. In silico analyses were performed to
provide further comprehension of the feasibility for predicting successful capsid formation. In contrast to previous work,
we show that common in silico predictions do not ensure assembly into particles. We included new considerations regarding
capsid formation of HBc fusion proteins. Not only the primary sequence and the length of the inserts seem important, also
the rigidity, the distance between the N and the C-terminus and the presence of cysteines, which could form disulphide bonds,
could influence proper capsid formation. Furthermore, new conformational insights were formulated when linkers were added
to create extra flexibility of the chimeric particles. Different hypotheses were suggested to clarify the obtained results.
To this extent, the addition of glycine-rich linkers could lower high rigidity of the insert, removal of the strain of the
core protein or ease interaction between the HBc and the insert. Finally, we observed specific changes in capsid formation
properties when longer linkers were used. These findings have not been reported before in this and other virus-like particle
carriers. In this study, we also propose a new high-yield purification protocol for fusion proteins to be used in vaccination
experiments with the carrier protein or in comparative studies of particulate or non-particulate HBc fusion proteins. 相似文献
114.
Humans have developed a particular innate immunity system against African trypanosomes, and only two Trypanosoma brucei clones (T. b. gambiense, T. b. rhodesiense) can resist this defence and cause sleeping sickness. The main players of this immunity are the primate‐specific apolipoprotein L‐I (apoL1) and haptoglobin‐related protein (Hpr). These proteins are both associated with two serum complexes, a minor subfraction of HDLs and an IgM/apolipoprotein A‐I (apoA1) complex, respectively, termed trypanosome lytic factor (TLF) 1 and TLF2. Although the two complexes appear to lyse trypanosomes by the same mechanism, they enter the parasite through various modes of uptake. In case of TLF1 one uptake process was characterized. When released in the circulation, haemoglobin (Hb) binds to Hpr, hence to TLF1. In turn the TLF1–Hpr–Hb complex binds to the trypanosome haptoglobin (Hp)–Hb receptor, whose original function is to ensure haem uptake for optimal growth of the parasite. This binding triggers efficient uptake of TLF1 and subsequent trypanosome lysis. While Hpr is involved as TLF ligand, the lytic activity is due to apoL1, a Bcl‐2‐like pore‐forming protein. We discuss the in vivo relevance of this uptake pathway in the context of other potentially redundant delivery routes. 相似文献
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117.
Ikram Zaïdi Chantal Ebel Majdi Touzri Etienne Herzog Jean-Luc Evrard Anne Catherine Schmit Khaled Masmoudi Moez Hanin 《Plant molecular biology》2010,73(3):325-338
The regulation of plant signalling responses by Mitogen-Activated Protein Kinases (MAPKs)-mediated protein phosphorylation is well recognized. MAP kinase phosphatases (MKPs) are negative regulators of MAPKs in eukaryotes. We report here the identification and the characterization of TMKP1, the first wheat MKP (Triticum turgidum L. subsp. Durum). Expression profile analyses performed in two durum wheat cultivars showing a marked difference in salt and drought stress tolerance, revealed a differential regulation of TMKP1. Under salt and osmotic stress, TMKP1 is induced in the sensitive wheat variety and repressed in the tolerant one. A recombinant TMKP1 was shown to be an active phosphatase and capable to interact specifically with two wheat MAPKs (TMPK3 and TMPK6). In BY2 tobacco cells transiently expressing GFP::TMKP1, the fusion protein was localized into the nucleus. Interestingly, the deletion of the N-terminal non catalytic domain results in a strong accumulation of the truncated fusion protein in the cytoplasm. In addition, when expressed in BY2 cells, TMPK3 and TMPK6 fused to red fluorescent protein (RFP) were shown to be present predominantly in the nucleus. Surprisingly, when co-expressed with the N-terminal truncated TMKP1 fusion protein; both kinases are excluded from the nuclear compartment and accumulate in the cytoplasm. This strongly suggests that TMKP1 interacts in vivo with TMPK3 and TMPK6 and controls their subcellular localization. Taken together, our results show that the newly isolated wheat MKP might play an active role in modulating the plant cell responses to salt and osmotic stress responses. 相似文献
118.
O. Dauwalder A. -M. Freydière H. Meugnier Y. Benito M. Chomarat C. Ginevra P. Girardo M. -E. Reverdy J. Etienne G. Lina F. Vandenesch 《Bio Tribune Magazine》2010,36(1):6-11
Concomitant chemoradiotherapy can constitute an alternative to surgical approach in selected patients with bladder carcinoma. TNM staging, histological grading, renal and performance status represent the main prognostic factors useful to define the therapeutic strategy. Biological and genetic factors are still controversial in medical practice. The activation of oncogenes seems to characterize the initiation of superficial lowgrade tumors and the mutations in the tumor suppressor genes (TEN, TP53 and RB) appear as the most important biological features in the in situ and invasive tumors. The expression of p53, Rb, Bcl-2 has an obvious prognostic role but the published studies on molecular factors predictive of radiosensibility are heterogeneous. 相似文献
119.
James Breen Thomas Wicker Xiuying Kong Juncheng Zhang Wujun Ma Etienne Paux Catherine Feuillet Rudi Appels Matthew Bellgard 《BMC plant biology》2010,10(1):98
Background
The complexity of the wheat genome has resulted from waves of retrotransposable element insertions. Gene deletions and disruptions generated by the fast replacement of repetitive elements in wheat have resulted in disruption of colinearity at a micro (sub-megabase) level among the cereals. In view of genomic changes that are possible within a given time span, conservation of genes between species tends to imply an important functional or regional constraint that does not permit a change in genomic structure. The ctg1034 contig completed in this paper was initially studied because it was assigned to the Sr2 resistance locus region, but detailed mapping studies subsequently assigned it to the long arm of 3B and revealed its unusual features. 相似文献120.
Yao Potchoo Kpatcha Tchamdja Agnon Balogou Vincent P Pitche Innocent P Guissou Etienne K Kassang 《BMC clinical pharmacology》2010,10(1):11