全文获取类型
收费全文 | 970篇 |
免费 | 63篇 |
专业分类
1033篇 |
出版年
2022年 | 15篇 |
2021年 | 18篇 |
2020年 | 12篇 |
2019年 | 20篇 |
2018年 | 25篇 |
2017年 | 26篇 |
2016年 | 32篇 |
2015年 | 47篇 |
2014年 | 70篇 |
2013年 | 62篇 |
2012年 | 82篇 |
2011年 | 88篇 |
2010年 | 39篇 |
2009年 | 41篇 |
2008年 | 52篇 |
2007年 | 52篇 |
2006年 | 34篇 |
2005年 | 42篇 |
2004年 | 46篇 |
2003年 | 27篇 |
2002年 | 19篇 |
2001年 | 19篇 |
2000年 | 25篇 |
1999年 | 10篇 |
1998年 | 17篇 |
1997年 | 10篇 |
1996年 | 3篇 |
1995年 | 6篇 |
1994年 | 5篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 5篇 |
1990年 | 4篇 |
1989年 | 8篇 |
1988年 | 6篇 |
1985年 | 5篇 |
1984年 | 5篇 |
1983年 | 4篇 |
1982年 | 3篇 |
1978年 | 4篇 |
1977年 | 2篇 |
1975年 | 2篇 |
1971年 | 2篇 |
1968年 | 3篇 |
1966年 | 3篇 |
1892年 | 2篇 |
1889年 | 3篇 |
1888年 | 2篇 |
1882年 | 2篇 |
1879年 | 3篇 |
排序方式: 共有1033条查询结果,搜索用时 0 毫秒
11.
12.
H.T. Nguyen S. Kim N.H. Yu A.R. Park H. Yoon C.-H. Bae J.H. Yeo I.S. Kim J.-C. Kim 《Journal of applied microbiology》2019,126(3):894-904
13.
Vilizzi Lorenzo Copp Gordon H. Adamovich Boris Almeida David Chan Joleen Davison Phil I. Dembski Samuel Ekmekçi F. Güler Ferincz Árpád Forneck Sandra C. Hill Jeffrey E. Kim Jeong-Eun Koutsikos Nicholas Leuven Rob S. E. W. Luna Sergio A. Magalhães Filomena Marr Sean M. Mendoza Roberto Mourão Carlos F. Neal J. Wesley Onikura Norio Perdikaris Costas Piria Marina Poulet Nicolas Puntila Riikka Range Inês L. Simonović Predrag Ribeiro Filipe Tarkan Ali Serhan Troca Débora F. A. Vardakas Leonidas Verreycken Hugo Vintsek Lizaveta Weyl Olaf L. F. Yeo Darren C. J. Zeng Yiwen 《Reviews in Fish Biology and Fisheries》2019,29(3):529-568
Reviews in Fish Biology and Fisheries - The freshwater Fish Invasiveness Screening Kit (FISK) has been applied in 35 risk assessment areas in 45 countries across the six inhabited continents (11... 相似文献
14.
Cui Heqing Song Myoung Chong Lee Ji Young Yoon Yeo Joon 《Journal of industrial microbiology & biotechnology》2019,46(12):1707-1713
Journal of Industrial Microbiology & Biotechnology - Methylated flavonoids possess improved bioactivities compared to their unmethylated counterparts. In this study, for the efficient... 相似文献
15.
16.
Yeo Cho Yoon Beom Hee Ahn Jin Woo Min Kyung Real Lee Sang Hoon Park Hee Cheol Kang 《Current issues in molecular biology》2022,44(2):845
Human hair follicle dermal papilla cells (HFDPCs) located in hair follicles (HFs) play a pivotal role in hair follicle morphogenesis, hair cycling, and hair growth. Over the past few decades, probiotic bacteria (PB) have been reported to have beneficial effects such as improved skin health, anti-obesity, and immuno-modulation for conditions including atopic dermatitis and inflammatory bowel disease (IBD). PB can secrete 50~150 nm sized extracellular vesicles (EVs) containing microbial DNA, miRNA, proteins, lipids, and cell wall components. These EVs can regulate communication between bacteria or between bacteria and their host. Although numerous biological effects of PB-EVs have been reported, the physiological roles of Leuconostoc holzapfelii (hs-Lh), which is isolated from human scalp tissue, and the extracellular vesicles derived from them (hs-LhEVs) are largely unknown. Herein, we investigated the effects of hs-LhEVs on hair growth in HFDPCs. We show that hs-LhEVs increase cell proliferation, migration, and regulate the cell cycle. Furthermore, hs-LhEVs were found to modulate the mRNA expression of hair-growth-related genes in vitro. These data demonstrate that hs-LhEVs can reduce apoptosis by modulating the cell cycle and promote hair growth by regulation via the Wnt/β-catenin signal transduction pathway. 相似文献
17.
18.
In many organisms, trehalose protects against several environmental stresses, such as heat, desiccation, and salt, probably by stabilizing protein structures and lipid membranes. Trehalose synthesis in yeast is mediated by a complex of trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate phosphatase (TPS2). In this study, genes encoding TPS1 and TPS2 were isolated from Zygosaccharomyces rouxii (designated ZrTPS1 and ZrTPS2, respectively). They were functionally identified by their complementation of the tps1 and tps2 yeast deletion mutants, which are unable to grow on glucose medium and with heat, respectively. Full-length ZrTPS1 cDNA is composed of 1476 nucleotides encoding a protein of 492 amino acids with a molecular mass of 56 kDa. ZrTPS2 cDNA consists of 2843 nucleotides with an open reading frame of 2700 bp, which encodes a polypeptide of 900 amino acids with a molecular mass of 104 kDa. The amino acid sequence encoded by ZrTPS1 has relatively high homology with TPS1 of Saccharomyces cerevisiae and Schizosaccharomyces pombe, compared with TPS2. Western blot analysis showed that the antibody against S. cerevisiae TPS1 recognizes ZrTPS1. Under normal growth conditions, ZrTPS1 and ZrTPS2 were highly and constitutively expressed, unlike S. cerevisiae TPS1 and TPS2. Salt stress and heat stress reduced the expression of the ZrTPS1 and ZrTPS2 genes, respectively. 相似文献
19.
In yeast, trehalose-6-phosphate synthase is a key enzyme for trehalose biosynthesis, encoded by the structural gene TPS1. Trehalose affects sugar metabolism as well as osmoprotection against several environmental stresses, such as heat and desiccation. The TPS1 gene of Saccharomyces cerevisiae was engineered under the control of the CaMV 35S promoter for constitutive expression in transgenic potato plants by Ti-plasmid of Agrobacterium-mediated transformation. The resulting TPS1 transgenic potato plants exhibited various morphological phenotypes in culture tubes, ranging from normal to severely retarded growth, including dwarfish growth, yellowish lancet-shaped leaves, and aberrant root development. However, the plants recovered from these negative growth effects when grown in a soil mixture. The TPS1 transgenic potato plants showed significantly increased drought resistance. These results suggest that the production of trehalose not only affects plant development but also improves drought tolerance. 相似文献
20.