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101.
Motomi Ito Kuniaki Watanabe Yoko Kita Takayuki Kawahara DJ Crawford Tetsukazu Yahara 《Journal of plant research》2000,113(1):79-89
Eupatorium were examined by sequencing the internal transcribed spacers (ITS) of nuclear ribosomal DNA and restriction site analysis
of chloroplast DNA. Molecular data provided strong evidence that (1) this genus originated in North America, (2) the genus
diverged into three morphological species groups, Eutrochium, Traganthes and Uncasia in North America, and (3) one of the North American Uncasia lineages migrated into temperate Europe and eastern Asia over the Bering land bridge. The estimated divergence times support
a late Miocene to early Pliocene migration from North America to Eurasia via the Bering land bridge. A European species was
sister to all of the eastern Asian species examined. The disjunct distribution pattern of the genus Eupatorium is incongruent with the classical Arcto-Tertiary geoflora concept.
Received 13 September 1999/ Accepted in revised form 4 January 2000 相似文献
102.
Several studies have indicated that olfactory responses are impeded by
amiloride. Therefore, it was of interest to see whether, and if so which,
olfactory epithelial cellular compartments have amiloride- sensitive
structures. Using ultrastructural methods that involved rapid freezing,
freeze-substitution and low temperature embedding of olfactory epithelia,
this study shows that, in the rat, this tissue is immunoreactive to
antibodies against amiloride sensitive Na(+)- channels. However, microvilli
of olfactory supporting cells, as opposed to receptor cilia, contained most
of the immunoreactive sites. Apices from which the microvilli sprout and
receptor cell dendritic knobs had much less if any of the
amiloride-antibody binding sites. Using a direct ligand-binding
cytochemical method, this study also confirms earlier ones that showed that
olfactory receptor cell cilia have Na+, K(+)-ATPase. It is proposed that
supporting cell microvilli and the receptor cilia themselves have
mechanisms, different but likely complementary, that participate in
regulating the salt concentration around the receptor cell cilia. In this
way, both structures help to provide the ambient mucous environment for
receptor cells to function properly. This regulation of the salt
concentration of an ambient fluid environment is a function that the
olfactory epithelium shares with cells of transporting epithelia, such as
those of kidney.
相似文献
103.
Transgenic Research - 相似文献
104.
105.
Genetic polymorphism in low-molecular-weight glutenin genes from Triticum aestivum, variety Chinese Spring 总被引:1,自引:0,他引:1
M. Benmoussa L.-P. Vézina M. Pagé S. Yelle S. Laberge 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(5):789-793
Low-molecular-weight (LMW) glutenin subunits consist mainly of two domains, one at the N- terminus which contains repeats
of short amino-acid motifs, and a non-repetitive one rich in cysteine, at the C- terminal region. In previous reports, polyacrylamide-gel
electrophoresis has been used to show that large size variation exists among LMW and HMW glutenin subunits, and it has been
suggested that deletions and insertions within the repetitive region are responsible for these variations in length. In this
study, PCR-amplification of genomic DNA (Triticum aestivum variety Chinese Spring) was used to isolate three full-length LMW glutenin genes: LMWG-MB1, LMWG-MB2 and LMWG-MB3. The deduced
amino-acid sequences show a high similarity between these ORFs, and with those of other LMW glutenin genes. Comparisons indicate
that LMWG-MB1 has probably lost a 12-bp fragment through deletion and that LMWG-MB1 and LMWG-MB2 have an insertion of 81 bp
within the repetitive domain. The current study has shown direct evidence that insertions and/or deletions provide a mechanistic
explanation for the allelic variation, and the resultant evolution, of prolamin genes. Single-base substitutions at identical
sites generate stop codons in both LMWG-MB2 and LMWG-MB3 indicating that these clones are pseudogenes.
Received: 7 May 1999 / Accepted: 17 June 1999 相似文献