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431.
Zurgil N Solodeev I Gilburd B Shafran Y Afrimzon E Avtalion R Shoenfeld Y Deutsch M 《Cell biochemistry and biophysics》2004,40(2):97-113
Cell death is a major event in the pathophysiology of atherosclerosis. Oxidized low-density lipoprotein (Ox-LDL), which plays
a key role in the atherogenesis, has a powerful cytotoxic effect and causes necrosis or apoptosis of different types of cells.
In the present work we studied the mechanism of cell death in two model systems: T lymphocytes and monocytes cell line, exposed
to Ox-LDL. Ox-LDL, but not native low-density lipoprotein (LDL), was found to be cytotoxic to both cell types in a dose and
time dependent manner. Apoptotic cell deat was analyzed by evaluating cell size, nucleus DNA content and plasma membrane asymmetry.
Early cytoplasmic condensation resulting from cell shrinkage was measured by monitoring fluorescence polarization (FP) of
fluorescein labeled cells. The redical scavenger superoxide dismutase (SOD), in a time- and dose-dependent manner, reduced
the apoptotic effect of Ox-LDL. Hyperpolarization of fluorescein-labeled cells preceded the appearance of phosphatidylserine
(PS) on the plasma membrane. This sensitive parameter for early apoptosis detected different cell death kinetics, as well
as varying sensitivity to the inhibitory effect of SOD in monocytes and lymphocytes. Such data suggest that reactive oxygen
species generation are involved, in Ox-LDL-induced apoptosis and that monocytes are more susceptible to cell death triggered
by oxidative stress. 相似文献
432.
SUMMARY: Given the positions of protein-coding genes along genomic sequence and probability values for protein alignments between genes, DAGchainer identifies chains of gene pairs sharing conserved order between genomic regions, by identifying paths through a directed acyclic graph (DAG). These chains of collinear gene pairs can represent segmentally duplicated regions and genes within a single genome or syntenic regions between related genomes. Automated mining of the Arabidopsis genome for segmental duplications illustrates the use of DAGchainer. 相似文献
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434.
Rice bioinformatics. analysis of rice sequence data and leveraging the data to other plant species 总被引:6,自引:0,他引:6
Yuan Q Quackenbush J Sultana R Pertea M Salzberg SL Buell CR 《Plant physiology》2001,125(3):1166-1174
435.
Priel-Halachmi S Ben-Dor I Shpungin S Tennenbaum T Molavani H Bachrach M Salzberg S Nir U 《The Journal of biological chemistry》2000,275(37):28902-28910
p94(fer) and p51(ferT) are two tyrosine kinases that share identical SH2 and kinase domains but differ in their N-terminal regions. To further explore the cellular functions of these two highly related tyrosine kinases, their subcellular distribution profiles and in vivo phosphorylation activity were followed using double immunofluorescence assay. When combined with immunoprecipitation analysis, this assay showed that p94(fer) can lead to the tyrosine phosphorylation and activation of Stat3 but not of Stat1 or Stat2. Native p94(fer) exerted this activity when residing in the cytoplasm. However, modified forms of p94(fer), which are constitutively nuclear, could also lead to the phosphorylation of Stat3. Endogenous Stat3 and p94(fer) co-immunoprecipitated with each other, thus proving the interaction of these two proteins in vivo. Unlike p94(fer), p51(ferT) did not induce the phosphorylation of Stat3 but led to the phosphorylation of other nuclear proteins. Replacing the unique 43-amino acid-long N-terminal tail of p51(ferT) with a parallel segment from the N-terminal tail of p94(fer) did not change the subcellular localization of p51(ferT) but enabled it to activate Stat3. Thus the different N-terminal sequences of p94(fer) and p51(ferT) can affect their ability to induce phosphorylation of Stat3 and most probably direct their different cellular functions. 相似文献
436.
Prediction of transcription terminators in bacterial genomes 总被引:25,自引:0,他引:25
437.
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439.
Neutralizing Activity of Broadly Neutralizing Anti-HIV-1 Antibodies against Primary African Isolates
440.