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51.
Three O-β-glucosides of procyanidin polymers (condensed tannins) have been isolated from the ripe fruits of Cydonia oblonga (quince) and the barks of Pinus brutia and Picea abies. 相似文献
52.
Selective visualization of 2D TLC of extracts of Phyllocladus species with vanillin—hydrochloric acid showed a characteristic pattern of constituents attributable to catechin, epicatechin and phylloflavan, a new flavanoid compound. Optical rotation measurements showed that while catechin and epicatechin are of the normal type with the 2R configurations, the flavan moiety in phylloflavan is of the opposite 2S configuration. Spectroscopic data of the new compound and its hydrolysis products showed phylloflavan to be ent-epicatechin-3-δ-(3,4-dihydroxyphenyl)-β-hydroxypentanoate. 相似文献
53.
H L Yeap G Ra?i? N M Endersby-Harshman S F Lee E Arguni H Le Nguyen A A Hoffmann 《Heredity》2016,116(3):265-276
Wolbachia is the most widespread endosymbiotic bacterium of insects and
other arthropods that can rapidly invade host populations. Deliberate releases of
Wolbachia into natural populations of the dengue fever mosquito,
Aedes aegypti, are used as a novel biocontrol strategy for dengue
suppression. Invasion of Wolbachia through the host population relies on
factors such as high fidelity of the endosymbiont transmission and limited
immigration of uninfected individuals, but these factors can be difficult to measure.
One way of acquiring relevant information is to consider mitochondrial DNA (mtDNA)
variation alongside Wolbachia in field-caught mosquitoes. Here we used
diagnostic mtDNA markers to differentiate infection-associated mtDNA haplotypes from
those of the uninfected mosquitoes at release sites. Unique haplotypes associated
with Wolbachia were found at locations outside Australia. We also performed
mathematical and qualitative analyses including modelling the expected dynamics of
the Wolbachia and mtDNA variants during and after a release. Our analyses
identified key features in haplotype frequency patterns to infer the presence of
imperfect maternal transmission of Wolbachia, presence of immigration and
possibly incomplete cytoplasmic incompatibility. We demonstrate that ongoing
screening of the mtDNA variants should provide information on maternal leakage and
immigration, particularly in releases outside Australia. As we demonstrate in a case
study, our models to track the Wolbachia dynamics can be successfully
applied to temporal studies in natural populations or Wolbachia release
programs, as long as there is co-occurring mtDNA variation that differentiates
infected and uninfected populations. 相似文献
54.
Lai Yeap Foo 《Phytochemistry》1982,21(7):1741-1746
The hydrogenolysis of Photinia glabrescens proanthocyanidin polymer was investigated. Among the low MW products identified were phloroglucinol, catechin, epicatechin, 1 - (3, 4 - dihydroxyphenyl) - 3 - (2, 4, 6 -trihydroxyphenyl) propan - 2 - ol and the procyanidin dimers B1 and B2. Initial reaction products may be related to the known structure of the polymer. The yield of the ethyl acetate-soluble fraction reached a maximum after about 3 hr of hydrogenation and the major oligomeric fractions had significantly lower MWs than the parent polymer. 相似文献
55.
L. Yeap Foo William T. Jones Lawrence J. Porter Vincent M. Williams 《Phytochemistry》1982,21(4):933-935
The structure of the condensed tannins of the most common fodder legumes is described. The number- (n) and weight-average (w ) MW of the polymers have been determined and most legume tannins have an approximately normal distribution of MWs with n values of 2000–4000. 相似文献
56.
Genetic relationship between Coptotermes gestroi and Coptotermes vastator (Isoptera: Rhinotermitidae) 总被引:1,自引:0,他引:1
The phylogenetic relationship of Coptotermes gestroi (Wasmann) and Coptotermes vastator Light (Isoptera: Rhinotermitidae) was determined using DNA sequence comparisons of mitochondrial genes. Partial sequences of the ribosomal RNA small subunit 12S, ribosomal RNA large subunit 16S, and mitochondrial COII were obtained from nine populations of C. gestroi from South East Asia (Malaysia, Singapore, Thailand, and Indonesia) and four populations of C. vastator from the Philippines and Hawaii. In addition, four populations of Coptotermes formosanus Shiraki and Globitermes sulphureus (Haviland) were used as the outgroups. Consensus sequences were obtained and aligned. C. vastator and C. gestroi are synonymous, based on high sequence homology across the 12S, 16S, and COII genes. The interspecific pairwise sequence divergence, based on Kimura 2-parameter model between C. gestroi and C. vastator, varied only up to 0.80%. Morphometric measurements of 16 characteristics revealed numerous overlaps between the examined individuals of both species. Based on the molecular phylogenetics and morphometric data, it is proposed that C. vastator is a junior synonym of C. gestroi. 相似文献
57.
Spencer P Sivakumaran S Fraser K Foo LY Lane GA Edwards PJ Meagher LP 《Phytochemical analysis : PCA》2007,18(3):193-203
An acetone:water (7:3) extract obtained from the leaves of Rumex obtusifolius was fractionated into procyanidin oligomer and polymer fractions using a linear gradient and a simple step method on Sephadex LH-20. The chemical characteristics of the procyanidin fractions were studied by 13C-NMR spectroscopy, acid-catalysed degradation in the presence of benzyl mercaptan, matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) MS and electrospray ionisation (ESI) MS. The 13C-NMR showed that the polymer fraction consisted predominantly of procyanidin polymers, some with galloyl groups attached. The thiolysis reaction products indicated a mean degree of polymerisation (DP) of 4.3 for the step method, and a range of 2.3-8.2 mean DP for the gradient fractionation, with epicatechin as the most abundant flavan-3-ol extension unit, while the terminal units consisted of equal proportions of catechin, epicatechin and epicatechin gallate. Singly charged ions observed in MALDI-TOF/MS showed a range of oligomeric procyanidins and their polygalloyl derivatives. These species (in the range DP 2-7) were also observed by ESI/MS but the spectra were more complex due to overlapping multiply charged ions. Isolation of oligomers from the Sephadex LH-20 fraction by chromatography on polyamide and C18 yielded B1, B2, B3 and B7 dimers, an A-type trimer and a B2 3,3'-O-digallate. 相似文献
58.
Variation in antimicrobial action of proanthocyanidins from Dorycnium rectum against rumen bacteria 总被引:1,自引:0,他引:1
Sivakumaran S Molan AL Meagher LP Kolb B Foo LY Lane GA Attwood GA Fraser K Tavendale M 《Phytochemistry》2004,65(17):2485-2497
The proanthocyanidin polymer fractions of the leaves of the forage legume Dorycnium rectum were analysed by acid catalysis with benzyl mercaptan, NMR and ES-MS. The results showed that D. rectum differs from other temperate proanthocyanidin-containing forage legumes in that the range of polymers extends up to very high degrees of polymerisation. Three fractions were characterised as low, medium, and high molecular weight proanthocyanidin fractions with mean degree of polymerisations of 10.3, 41 and 127, respectively. Epigallocatechin was the most abundant extension unit and the terminating flavan-3-ols comprised largely catechin and gallocatechin units in equal proportions. Formation of thiolyated dimer products showed the interflavan-linkages of the lower molecular weight proanthocyanidins to be predominantly C4-->C8 with a small amount of C4-->C6. ES-MS spectra distinguished lower from higher polymeric proanthocyanidins from M2- to M8(2)-. The antibacterial activity of proanthocyanidin fractions against pure cultures of microbes selected from the ruminal population to represent fibre degrading, proteolytic and hyper ammonia producing bacteria in broth culture was evaluated. The activity of proanthocyanidin fractions against Clostridium aminophilum, Butyrivibrio fibrisolvens and Clostridium proteoclasticum was significantly dependent on their structure but not so against Ruminococcus albus and Peptostreptococcus anaerobius. The latter observation was unique in that they were sensitive to all proanthocyanidin fractions evaluated, even at the lowest concentration (100 microg/ml). The results suggest the effects of the extractable proanthocyanidins on rumen microbes should be considered when evaluating an alternative proanthocyanidin-containing forage source for ruminants, such as D. rectum. 相似文献
59.
KL Lim SD Jazayeri SK Yeap NB Mohamed Alitheen MH Bejo A Ideris AR Omar 《BMC veterinary research》2012,8(1):132
ABSTRACT: BACKGROUND: DNA vaccines offer several advantages over conventional vaccines in the development of effective vaccines against avian influenza virus (AIV). However, one of the limitations of the DNA vaccine in poultry is that it induces poor immune responses. In this study, chicken interleukin (IL) -15 and IL-18 were used as genetic adjuvants to improve the immune responses induced from the H5 DNA vaccination in chickens. The immunogenicity of the recombinant plasmid DNA was analysed based on the antibody production, T cell responses and cytokine production, following inoculation in 1-day-old (Trial 1) and 14-day-old (Trial 2) specific-pathogen-free chickens. Hence, the purpose of the present study was to explore the role of chicken IL-15 and IL-18 as adjuvants following the vaccination of chickens with the H5 DNA vaccine. RESULTS: The overall HI antibody titer in chickens immunized with pDis/H5 + pDis/IL-15 was higher compared to chickens immunized with pDis/H5 (p < 0.05). The findings revealed that the inoculation of the 14-day-old chickens exhibited a shorter time to achieve the highest HI titer in comparison to the inoculation of the 1-day-old chickens. The cellular immunity was assessed by the flow cytometry analysis to enumerate CD4+ and CD8+ T cells in the peripheral blood. The chickens inoculated with pDis/H5 + pDis/IL-15 demonstrated the highest increase in CD4+ T cells population relative to the control chickens. However, this study revealed that pDis/H5 + pDis/IL-15 was not significant (P > 0.05) in inducing CD8+ T cells. Meanwhile, with the exception of Trial 1, the flow cytometry results for Trial 2 demonstrated that the pDis/H5 + pDis/IL-18 inoculated group was able to trigger a higher increase in CD4+ T cells than the pDis/H5 group (P < 0.05). On the other hand, the pDis/H5 + pDis/IL-18 group was not significant (P > 0.05) in modulating CD8+ T cells population in both trials. The pDis/H5 + pDis/IL-15 inoculated group showed the highest IL-15 gene expression in both trials compared to other inoculated groups (P < 0.05). Similar results were obtained for the IL-18 expression where the pDis/H5 + pDis/IL-18 groups in both trials (Table 8) were significantly higher compared to the control group (P < 0.05). However, the expressions of other cytokines remained low or undetected by GeXP assay. CONCLUSIONS: This study shows the diverse immunogenicity of pDis/H5 co-administered with chicken IL-15 and IL-18,with pDis/H5 + pDis/IL-15 being a better vaccine candidate compared to other groups. 相似文献
60.
Tamilselvan Subramani Swee Keong Yeap Wan Yang Ho Chai Ling Ho Abdul Rahman Omar Suraini Abdul Aziz Nik Mohd. Afizan Nik Abd. Rahman Noorjahan Banu Alitheen 《Journal of cellular and molecular medicine》2014,18(2):305-313
Vitamin C is generally thought to enhance immunity and is widely taken as a supplement especially during cancer treatment. Tamoxifen (TAM) has both cytostatic and cytotoxic properties for breast cancer. TAM engaged mitochondrial oestrogen receptor beta in MCF‐7 cells and induces apoptosis by activation of pro‐caspase‐8 followed by downstream events, including an increase in reactive oxygen species and the release of pro‐apoptotic factors from the mitochondria. In addition to that, TAM binds with high affinity to the microsomal anti‐oestrogen‐binding site and inhibits cholesterol esterification at therapeutic doses. This study aimed to investigate the role of vitamin C in TAM‐mediated apoptosis. Cells were loaded with vitamin C by exposure to dehydroascorbic acid, thereby circumventing in vitro artefacts associated with the poor transport and pro‐oxidant effects of ascorbic acid. Pre‐treatment with vitamin C caused a dose‐dependent attenuation of cytotoxicity, as measured by acridine‐orange/propidium iodide (AO/PI) and Annexin V assay after treatment with TAM. Vitamin C dose‐dependently protected cancer cells against lipid peroxidation caused by TAM treatment. By real‐time PCR analysis, an impressive increase in FasL and tumour necrosis factor‐α (TNF‐α) mRNA was detected after TAM treatment. In addition, a decrease in mitochondrial transmembrane potential was observed. These results support the hypothesis that vitamin C supplementation during cancer treatment may detrimentally affect therapeutic response. 相似文献