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381.
382.
Yayoi Kaneko Kyohei Shimoda Rafael Ayala Yukina Goto Silvia Panico Xiaodong Zhang Hisao Kondo 《The EMBO journal》2021,40(9)
p97ATPase‐mediated membrane fusion is required for the biogenesis of the Golgi complex. p97 and its cofactor p47 function in soluble N‐ethylmaleimide‐sensitive factor (NSF) attachment protein receptor (SNARE) priming, but the tethering complex for p97/p47‐mediated membrane fusion remains unknown. In this study, we identified formiminotransferase cyclodeaminase (FTCD) as a novel p47‐binding protein. FTCD mainly localizes to the Golgi complex and binds to either p47 or p97 via its association with their polyglutamate motifs. FTCD functions in p97/p47‐mediated Golgi reassembly at mitosis in vivo and in vitro via its binding to p47 and to p97. We also showed that FTCD, p47, and p97 form a big FTCD‐p97/p47‐FTCD tethering complex. In vivo tethering assay revealed that FTCD that was designed to localize to mitochondria caused mitochondria aggregation at mitosis by forming a complex with endogenous p97 and p47, which support a role for FTCD in tethering biological membranes in cooperation with the p97/p47 complex. Therefore, FTCD is thought to act as a tethering factor by forming the FTCD‐p97/p47‐FTCD complex in p97/p47‐mediated Golgi membrane fusion. 相似文献
383.
Yayoi Takeuchi Hiromitsu Samejima Michiko Nakagawa Bibian Diway Tohru Nakashizuka 《Journal of plant research》2010,123(2):249-259
This study investigated whether reproductive success is affected by the intensity of neighborhood aggregation of adults in
the tropical tree Shorea laxa. We focused on three processes in the early reproductive stages: seed maturation; seed survival (categorizing sound seed,
predation by insects and predation by vertebrates) in pre-seed dispersal; and seedling survival in the post-seed dispersal
stage. We used a model selection procedure to examine the aggregation effect on reproductive success. The intensity of neighborhood
aggregation was represented by the neighborhood aggregation index, which contains the adult number within a specific radius
and the distances to neighboring adults (weight of proximity). Then, we evaluated the models exhaustively with the aggregation
index having different scales (radius and weight of proximity) to assess the scale on which aggregation had significant effects.
In particular, the best effective neighborhood scale, which is defined as the scale of the index in the model with minimum
Akaike information criterion, was examined to compare those scales among processes. We found that the probability of seed
maturation, seed survival and seedling survival decreased with the aggregation index at specific scales. This suggests that
aggregation influenced reproductive success negatively in both the pre- and post-seed dispersal stages. However, the selected radii differed among processes: >200 and 130 m in pre- and post-seed dispersal stages, respectively.
The selected weight of proximity also seemed to have a weak effect on all processes and was not different among processes. 相似文献
384.