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排序方式: 共有534条查询结果,搜索用时 156 毫秒
101.
Takeshi Obayashi Takashi Okegawa Yuko Sasaki-Sekimoto Hiroshi Shimada Tatsuru Masuda Erika Asamizu Yasukazu Nakamura Daisuke Shibata Satoshi Tabata Ken-ichiro Takamiya Hiroyuki Ohta 《DNA research》2004,11(1):11-25
The distinctive features of plant organs are primarily determined by organ-specific gene expression. We analyzed the expression specificity of 8809 genes in 7 organs of Arabidopsis using a cDNA macroarray system. Using relative expression (RE) values between organs, many known and unknown genes specifically expressed in each organ were identified. We also analyzed the organ specificity of various gene groups using the GRE (group relative expression) value, the average of the REs of all genes in a group. Consequently, we found that many gene groups even ribosomal protein genes, have strong organ-specific expression. Clustering of the expression profiles revealed that the 8809 genes were classified into 9 major categories. Although 3451 genes were clustered into the largest category, which showed constitutive gene expression, 266 and 1005 genes were found to be root- and silique-specific genes, respectively. By this clustering, particular gene groups which showed multi-organ-specific expression profiles, such as bud-flower-specific, stem-silique-specific or bud-flower-root-specific profiles, could be effectively identified. From these results, major features of plant organs could be characterized by their distinct profiles of global gene expression. These data of organ-specific gene expression are available at our web site: Arabidopsis thaliana Tissue-Specific Expression Database, ATTED (http://www.atted.bio.titech.ac.jp/). 相似文献
102.
Kanako Sasaki Hideki Takase Shuhei Matsuyama Hironori Kobayashi Hironori Matsuo Gen Ikoma 《Bioscience, biotechnology, and biochemistry》2016,80(12):2376-2382
Linalool is an important compound that contributes to the floral aroma in wines. This study showed the effect of light exposure on linalool accumulation in berries. The grape bunches were covered with films that block the full light spectrum (Shade) and the UV spectrum (UV-block), and a transparent film (Control). The linalool content was significantly higher in juice from Control-covered berries than in juice from Shade- and UV-block-covered berries, and the expression levels of the representative genes in linalool biosynthesis in Shade- and UV-block-covered berries were markedly lower than in Control-covered berries. These findings suggest that exposing berries to light is essential for linalool biosynthesis. To reflect sunlight onto grape clusters, reflective sheets were placed on the ground of a vineyard. The linalool content in berries exposed to sunlight reflected from the reflective sheets was higher than those in the control. 相似文献
103.
Yasukazu Saitoh Minoru Ikeshima Naho Kawasaki Aoi Masumoto Nobuhiko Miwa 《Free radical research》2016,50(4):385-395
In our previous study, we demonstrated that combined treatment with hydrogen (H2) and platinum nanocolloid (Pt-nc) exerted markedly antiproliferative effects on cancer cells compared with each treatment alone. However, because the related mechanisms remain unclear, we investigated carcinostatic mechanisms of the combined treatment with H2 + Pt-nc. Significant suppression of cell proliferation was confirmed at 52?h following combined treatment, and the similar effect was also observed by the 30- or 40-min transient treatment with H2?+?Pt-nc. The transient treatments led to changes in cell size and morphology, loss of microvilli, and apoptosis-like cell death at 120 h after treatment. Moreover, transient combined treatment with H2?+?Pt-nc induced cell-cycle arrest, as reflected by decreased proportions of G1-phase cells and accumulation of G2/M-phase cells. In contrast, intracellular peroxide levels were temporarily and significantly increased immediately after H2?+?Pt-nc treatment but not after treatment with H2 or Pt-nc alone. Additionally, combined treatment-induced carcinostatic effects were significantly diminished in the presence of catalase, and marked hydrogen peroxide (H2O2) generation was confirmed after mixing Pt-nc into cell culture media containing a high concentration of H2. These changes are in agreement with the results that carcinostatic effects were induced after only 40 min of treatment with H2?+?Pt-nc. Thus, transient and marked generation of H2O2 is responsible for the carcinostatic effects of combined treatment with H2?+?Pt-nc. 相似文献
104.
105.
The performance of an ultrafiltration hollow-fiber reactor, in which the enzymatic synthesis of glucose 6-phosphate from glucose and cofactor ATP and the enzymatic regeneration of ATP from ADP and acetyl phosphate are performed simultaneously, was analyzed theoretically. A simple analytical model in which the liquid flowing in the fiber tubes is assumed to be plug flow, and the radial concentration gradients in the tube and shell sides are both neglected, could simulate the reactor performance with satisfactory accuracy. The simulation elucidated the effects of the reactor configurations and various operational conditions on glucose conversion, ATP recycle number, and space-time yield. If the fiber tubes, through which the permeability of the relevant components such as substrates is high, were packed as much as possible in the reactor, good reactor performance could be expected. Furthermore, with a sufficiently high enzyme concentration, low ATP concentration in the feed solution, and appropriate space velocity, good space-time yield with high glucose conversion and with very high ATP recycle number is theoretically possible. 相似文献
106.
M Murakami M Takase K Yoshino K Odake 《Biochemical and biophysical research communications》1971,44(4):797-803
Labeled cyclic AMP and other adenine nucleotides in human platelets were distinctly separated by means of thin-layer chromatography. In lysates of human platelets, ATP was decomposed following the major route: ATP→ADP→AMP→IMP→inosine→hypoxanthine. In contrast, cyclic AMP synthesis occurred rapidly following the breakdown of ATP and leveled off after 30–60 min of incubation. Cyclic AMP synthesis in platelet lysates was 1.02 ± 0.39 nanomoles/hr/mg protein. The level of cyclic AMP formed was related to the 5′-AMP level, although the former did not exceed 5 % of the latter. 相似文献
107.
Yukio Kato Norimitsu Nasu Toshiyuki Takase Yasushi Daikuhara Fujio Suzuki 《Experimental cell research》1980,125(1):167-174
The effect of hormones influencing cartilage metabolism on the growth of chondrocytes isolated from rabbit and rat ribs was investigated in serum-free medium. Insulin supported growth of the cells slightly, whereas calcitonin and parathyroid hormone did not. On the other hand, multiplication-stimulating activity (MSA), a substance partially purified from serum-free medium conditioned by the growth of Buffalo rat liver cells, markedly induced not only proliferation of the chondrocytes but also their synthesis of acid mucopolysaccharides, the characteristic cartilage phenotype, in serum-free medium. These cells maintained this specialized cellular function of differentiated chondrocytes for at least 21 days in serum-free medium. A combination of MSA and other hormones, such as insulin, calcitonin, and parathyroid hormone was even more effective in stimulating sulfation of glycosaminoglycans. These rabbit and rat chondrocytes cultured in completely defined medium seem to be a good experimental system for studies on chondrogenesis and endochondral ossification. 相似文献
108.
Tomoo Enomoto Kiyoaki Matsubayashi Yasukazu Nagato Mayumi Nakano 《Primates; journal of primatology》1995,36(3):411-422
The degenerating pattern of spermatogenic cells in the seminiferous tubule of Japanese macaques was studied to clarify a relationship
between seasonal changes of reproductive performances and cytological findings in the Japanese macaque. For light microscopy,
testis samples were obtained from five adult animals by biopsy in April (nonmating season) and October (mating season). For
electron microscopy, specimens from four additional macaques were used. Degenerating cells were found in all steps of spermatogenesis.
In stages I to V of the cycle of the seminiferous epithelium, morphologically atypical pachytene spermatocytes were observed
in 14.7 and 10.0% of the cells in the nonmating and mating seasons, respectively, although the difference in percentage was
not significant. Mature spermatids with atypical features in those stages occupied 59.6% of the cells in the nonmating season,
which significantly decreased to 34.1% in the mating season. These results imply that the seasonal change of sperm production
is related, at least in part, to the process of degeneration of the spermatogenic cells in this species. 相似文献
109.
110.
Hisano Hiroshi; Sato Shusei; Isobe Sachiko; Sasamoto Shigemi; Wada Tsuyuko; Matsuno Ai; Fujishiro Tsunakazu; Yamada Manabu; Nakayama Shinobu; Nakamura Yasukazu; Watanabe Satoshi; Harada Kyuya; Tabata Satoshi 《DNA research》2007,14(6):271-281
We generated a high-density genetic linkage map of soybean usingexpressed sequence tag (EST)-derived microsatellite markers.A total of 6920 primer pairs (10.9%) were designed to amplifysimple sequence repeats (SSRs) from 63 676 publicly availablenon-redundant soybean ESTs. The polymorphism of two parent plants,the Japanese cultivar Misuzudaizu and the Chineseline Moshidou Gong 503, were examined using 10%polyacrylamide gel electrophoresis. Primer pairs showing polymorphismwere then used for genotyping 94 recombinant inbred lines (RILs)derived from a cross between the parents. In addition to previouslyreported markers, 680 EST-derived microsatellite markers wereselected and subjected to linkage analysis. As a result, 935marker loci were mapped successfully onto 20 linkage groups,which totaled 2700.3 cM in length; 693 loci were detected usingthe 668 EST-derived microsatellite markers developed in thisstudy, the other 242 loci were detected with 105 RFLP markers,136 genome-derived microsatellite markers, and one phenotypicmarker. We examined allelic variation among 23 soybean cultivars/linesand a wild soybean line using 668 mapped EST-derived microsatellitemarkers (corresponding to 686 marker loci), in order to determinethe transferability of the markers among soybean germplasms.A limited degree of macrosynteny was observed at the segmentallevel between the genomes of soybean and the model legume Lotusjaponicus, which suggests that considerable genome shufflingoccurred after separation of the species and during establishmentof the paleopolyploid soybean genome. 相似文献