全文获取类型
收费全文 | 908篇 |
免费 | 46篇 |
国内免费 | 2篇 |
出版年
2022年 | 7篇 |
2021年 | 12篇 |
2020年 | 7篇 |
2019年 | 8篇 |
2018年 | 17篇 |
2017年 | 10篇 |
2016年 | 15篇 |
2015年 | 29篇 |
2014年 | 39篇 |
2013年 | 74篇 |
2012年 | 55篇 |
2011年 | 65篇 |
2010年 | 27篇 |
2009年 | 30篇 |
2008年 | 40篇 |
2007年 | 57篇 |
2006年 | 57篇 |
2005年 | 45篇 |
2004年 | 59篇 |
2003年 | 54篇 |
2002年 | 66篇 |
2001年 | 11篇 |
2000年 | 8篇 |
1999年 | 8篇 |
1998年 | 12篇 |
1997年 | 9篇 |
1996年 | 12篇 |
1995年 | 10篇 |
1994年 | 8篇 |
1993年 | 5篇 |
1992年 | 7篇 |
1991年 | 11篇 |
1990年 | 8篇 |
1989年 | 5篇 |
1988年 | 11篇 |
1987年 | 6篇 |
1986年 | 5篇 |
1985年 | 5篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1982年 | 9篇 |
1981年 | 11篇 |
1980年 | 2篇 |
1979年 | 4篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1969年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有956条查询结果,搜索用时 46 毫秒
21.
A new gobiid species,Acanthogobius insularis, is described from 88 specimens collected from Amami-oshima Island and Okinawa Island, Ryukyu Islands, southern Japan. The
species is distinguished from its congeners by the combination of dorsal and anal fin ray counts, vertebral counts, cephalic
sensory system patterns and coloration. 相似文献
22.
The localization of the antigen for monoclonal antibody 9F11-B-E4 was clarified by immuno-electron microscopy. The antigens were localized on the mitochondria and Golgi bodies in the male germ cells and on the secretory granules of various glands cells in the penis bulb and subepidermal parenchymal tissue of Phagocata vivida. The results of the interspecific cross-reaction tests with seven other freshwater triclads showed that these secretory granules are species-specific. A positive interspecific reaction was showed with Dugesia (family Dugesiidae), but not with Polycelis within the same family Planariidae which suggests the position of Phagocata within the Planariidae needs to be reassesed. 相似文献
23.
Akira Uchimura Toshiyuki Shimizu Masahiro Morita Hitomi Ueno Kazuhiro Motoki Hideaki Fukushima Takenori Natori Yasuhiko Koezuka 《Bioorganic & medicinal chemistry》1997,5(12):2245-2249
We compared the immunostimulatory effects of chemically synthesized α-galactosylceramides (α-GalCers), α-glucosylceramides (α-GluCers), 6″-monoglycosylated α-GalCer and 6″- or 4″-monoglycosylated α-GluCer and made the following observations: (1) the length of the fatty acid side chain in the ceramide portions greatly affects the immunostimulatory effects of α-GalCers and α-GluCers; (2) the configuration of the 4″-hydroxyl group of the inner pyranose moiety plays an important role in the immunostimulatory effects of monoglycosylated α-
-pyranosylceramides; (3) the free 4″-hydroxyl group of the inner pyranose of monoglycosylated α-
-pyranosylceramides plays a more important role in their immunostimulatory effects than the free 6″-hydroxyl group. 相似文献
24.
Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments 总被引:11,自引:0,他引:11
Nobuhisa Komai Tsutomu Nishizawa Yasuhiko Hayakawa Tomoaki Murotsu Kenichi Matsubara 《Molecular & general genetics : MGG》1982,186(2):193-203
Summary Various DNA subfragments were derived from miniF DNA by complete or partial PstI cleavage, and cloned in the plasmid vectors pBR322 or dv1. The recombinant plasmids obtained were introduced into an Escherichia coli minicell-producing strain, and the plasmid-coded proteins were radiolabeled and analyzed by gel electrophoresis. Six miniF-encoded proteins, larger than 11 000 daltons, were detected and their coding regions were mapped on the F plasmid genome. Three of them were assigned by taking into account the known nucleotide sequences (Murotsu et al. 1981; K. Yoshioka, personal communication). The coding directions of some proteins were determined by inserting the lac promotor into one of the recombinant plasmids and analyzing the increase in production of the proteins. The coding direction of the five proteins analyzed so far was uniform. Comparison of these results with a functional map of miniF suggested possible roles of the proteins. 相似文献
25.
Summary A patient with acute lymphoblastic leukemia (ALL) whose cells had L3 (Burkitt-type) morphology was found to have a variant translocation t(2;8)(p13;q24), that has been reported only in Burkitt lymphomas. This observation provides additional evidence for a close association of particular karyotypic abnormalities with both Burkitt-type ALL and Burkitt lymphoma. 相似文献
26.
27.
28.
Fumio Sawada Yasuhiko Miyauchi Hiroshi Tanaka Shinji Matsumoto 《Biochemical and biophysical research communications》1982,104(2):657-663
A novel method for the preparation of intact chromatin from the slime mold which retains the property of RNA synthesis is described. Preparations from G2-cells were highly active, while those from metaphase-cells were inactive. The plasmodial cells were disrupted by gentle homogenization on a polyethylene sieve in a neutral isotonic sucrose medium containing Mg++, deoxycholate and EGTA, a Ca++-chelating agent. The nuclei were lysed in a hypotonic buffer without use of EDTA and chromatin was precipitated by centrifugation after addition of Mg++. 相似文献
29.
Kohei Kawashima Eiji Watanabe Ken-ichi Isobe Michinori Ogura Ei-ichi Nagura Kazumasa Yamada Itsuro Sobue Kenji Mizoguchi Yasuhiko Ito Yoshiyuki Nagai Izumi Nakashima 《Cellular immunology》1982,67(2):279-286
During the course of immunization of (C3H × DBA/2)F1 mice (genotype H-2k/b) with L cell (H-2k/k)/L1210 leukemia cell (H-2d/d) hybrids and L1210 leukemia cells, some of them produced a good titer of anti-self-H-2 (H-2d) antibodies. Antigens recognized by this anti-self-H-2 antiserum were shown to be controlled by the H-2K-IA-IB-IJ-IE subregions of the H-2d but not H-2k nor H-2b haplotypes of parental as well as F1 origins and to have a tissue distribution identical to that of class 1 H-2 (H-2K/D) antigens. 相似文献
30.
Hiroaki Nakayama Koji Nakayama Ritsuko Nakayama Yasuhiko Kato 《Archives of microbiology》1982,131(4):308-312
Hydrophobic and charge-charge interactions of Salmonella typhimirium and Serratia marcescens were determined and related to their content of fimbriae and lipopolysaccharide (LPS). The cell surface structures were characterized with hydrophobic interaction chromatography (HIC), electrostatic interaction chromatography (ESIC) and particle electrophoresis measurements. The degree of interaction at the air-water interface was tested using a monolayered lipid film applied to an aqueous surface. The cell surface hydrophobicity of S. typhimurium in the presence of fimbriae was less in smooth than in rought bacteria. Examination of a series of rough mutants of S. typhimurium indicates that reduction of the O-side chain and core oligosaccharides was correlated with increased cell hydrophobicity. The enrichment factors at the air-water interface were significantly higher for fimbriated than for non-fimbriated S. typhimurium cells. Fimbriated S. marcescens cells were less hydrophobic and adhered to a lesser degree at the air-water surface than non-fimbriated counterparts. Electrophoretic measurements and adsorption to ion exchangers gives different information about the surface charge of bacteria. The latter technique gives the interaction between localized charged surfaces.Abbreviations HIC
hydrophobic interaction chromatography
- ESIC
electrostatic interaction chromatography
- LPS
lipopolysaccharide
- PBS
phosphate buffered saline solution 相似文献