全文获取类型
收费全文 | 4328篇 |
免费 | 240篇 |
专业分类
4568篇 |
出版年
2022年 | 24篇 |
2021年 | 53篇 |
2020年 | 29篇 |
2019年 | 32篇 |
2018年 | 54篇 |
2017年 | 53篇 |
2016年 | 74篇 |
2015年 | 134篇 |
2014年 | 162篇 |
2013年 | 235篇 |
2012年 | 247篇 |
2011年 | 265篇 |
2010年 | 178篇 |
2009年 | 164篇 |
2008年 | 253篇 |
2007年 | 280篇 |
2006年 | 250篇 |
2005年 | 228篇 |
2004年 | 253篇 |
2003年 | 241篇 |
2002年 | 245篇 |
2001年 | 97篇 |
2000年 | 77篇 |
1999年 | 82篇 |
1998年 | 54篇 |
1997年 | 46篇 |
1996年 | 34篇 |
1995年 | 44篇 |
1994年 | 28篇 |
1993年 | 39篇 |
1992年 | 64篇 |
1991年 | 54篇 |
1990年 | 53篇 |
1989年 | 41篇 |
1988年 | 37篇 |
1987年 | 37篇 |
1986年 | 38篇 |
1985年 | 36篇 |
1984年 | 29篇 |
1983年 | 21篇 |
1982年 | 24篇 |
1981年 | 16篇 |
1980年 | 21篇 |
1979年 | 20篇 |
1978年 | 18篇 |
1977年 | 16篇 |
1976年 | 11篇 |
1975年 | 11篇 |
1974年 | 13篇 |
1973年 | 9篇 |
排序方式: 共有4568条查询结果,搜索用时 15 毫秒
51.
Takuma Kobayashi Kunio Yasuda Masasuke Araki 《Development, growth & differentiation》2010,52(4):351-363
Dorsal and ventral specification in the early optic vesicle plays a crucial role in vertebrate ocular morphogenesis, and proper dorsal‐ventral polarity in the optic vesicle ensures that distinct structures develop in separate domains within the eye primordium. The polarity is determined progressively during development by coordinated regulation of extraocular dorsal and ventral factors. In the present study, we cultured discrete portions of embryonic chick brains by preparing anterior cephalon, anterior dorsal cephalon and anterior ventral cephalon, and clearly demonstrate that bone morphogenetic protein 4 (BMP4) and Sonic hedgehog (Shh) constitute a dorsal‐ventral signaling system together with fibroblast growth factor 8 (FGF8). BMP4 and Shh upregulate Tbx5 and Pax2, as reported previously, and at the same time Shh downregulates Tbx5, while BMP4 affects Pax2 expression to downregulate similarly. Shh induces Fgf8 expression in the ventral optic vesicle. This, in turn, determines the distinct boundary of the retinal pigmented epithelium and the neural retina by suppressing Mitf expression. The lens develops only when signals from both the dorsal and ventral regions come across together. Inverted deposition of Shh and BMP4 signals in organ‐cultured optic vesicle completely re‐organized ocular structures to be inverted. Based on these observations we propose a novel model in which the two signals govern the whole of ocular development when they encounter each other in the ocular morphogenic domain. 相似文献
52.
Hideyuki Kawabata Taro Katsura Eiji Kondo Nobuto Kitamura Shin Miyatake Yoshie Tanabe Takao Setoguchi Setsuro Komiya Kazunori Yasuda 《Journal of biomechanics》2009,42(15):2611-2615
The effect of stress deprivation on the tendon tissue has been an important focus in the field of biomechanics. However, less is known about the in vivo effect of stress deprivation on fibroblast apoptosis as of yet. This study was conducted to test a hypothesis that complete stress deprivation of the patellar tendon induces fibroblast apoptosis in vivo with activation of Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38) within 24 h after treatment. A total of 35 mature rabbits were divided into stress-shielded (n=15), sham-operated (n=15), and control (n=5) groups. To completely shield the patellar tendon from stress, we used an established surgical method. Animals were sacrificed at 24 h, and 2, 4, 7, and 14 days after the treatment. Tendon specimens underwent TUNEL assay and immunohistological examinations of active caspase-3, JNK, and p38. Both the number and the ratio of TUNEL-positive and caspase-3-positive cells were significantly greater (p<0.0001) in the stress-shielded group than in the sham group at 24 h, 2, 4, and 7 days. Concerning JNK and p38, both the number and the ratio were significantly greater (p<0.0001) in the stress-shielded group than in the sham group at 24 h, 2, and 4 days. This study demonstrated that complete stress deprivation induces fibroblast apoptosis in vivo with activation of JNK and p38 within 24 h. This fact suggested that the fibroblast apoptosis caused by stress deprivation is induced via the mitogen-activated protein kinase signaling pathway. 相似文献
53.
Yoko Nishikawa 《Plant Ecology》2009,202(2):337-347
I studied the relationship between seed-set patterns within inflorescences and temporal variations in light and pollinator
availabilities for 2 years in the spring ephemeral species Gagea lutea in a deciduous forest. Timing of canopy closure and seasonal trend of pollinator frequency did not synchronize with the annual
fluctuation in flowering phenology. In the early snowmelt year, seed-set success reflected the seasonal pollinator abundance
from early to middle flowering periods. In the late snowmelt year, however, seed-set rates were independent of pollinator
activity and decreased with canopy closing even after hand pollination. The restricted seed production by defoliation and
the increase in seed-set rates at the forest edge suggested that seed production was supported by current photosynthetic carbon
gain. Thus, annual fluctuations of reproductive success can explain the variation in flowering phenology within a population
although seasonal light deterioration would serve as a selective force for flowering in the early season. 相似文献
54.
Yoko Ino Yutaro Yamaoka Kiho Tanaka Kei Miyakawa Mayuko Nishi Yasuyoshi Hatayama Hirokazu Kimura Yayoi Kimura Akihide Ryo 《Proteomics》2023,23(11):2200334
Peptide tag systems are a robust biophysical and biochemical method that is widely used for protein detection and purification. Here, we developed a novel tag system termed “HiP4” (histidine plus four amino acids) whose epitope sequence comprises only seven amino acids (HHHDYDI) that partially overlap with the conventional 6x histidine tag (6xHis-tag). We produced a monoclonal antibody against the HiP4 tag that can be used in multiple immunoassays with high specificity and affinity. Using this system, we developed a tandem affinity purification (TAP) and mass spectrometry (TAP-MS) system for comprehensive protein interactome analysis. The integrated use of nickel bead purification followed by HiP4 tag immunoprecipitation made it possible to reduce nonspecific binding and improve selectivity, leading to the recovery of previously unrecognized proteins that interact with hepatitis B virus X (HBx) protein or TAR DNA-binding protein 43 (TARDBP or TDP-43). Our results indicate that this system may be viable as a simple and powerful tool for TAP-MS that can achieve low background and high selectivity in comprehensive protein–protein interaction analyses. 相似文献
55.
Go Suzuki Wataru Okada Yoko Yasutake Hidekazu Yamamoto Iwao Tanita Hiroshi Yamashita Takeshi Hayashibara Toshiaki Komatsu Toru Kanyama Masahito Inoue Masashi Yamazaki 《Restoration Ecology》2020,28(5):1172-1182
Larval recruitment is essential for sustaining coral communities and a fundamental tool in some interventions for reef restoration. To improve larval supply and post‐settlement survival in sexually assisted coral restoration efforts, an integrated in situ collector system, the larval cradle, was designed to collect spawned gametes then culture the resulting larvae until settled on artificial substrates. The final design of the larval cradle was cylindrical, a nylon mesh structure with a volume of 9 m3, suspended in the sea and extending vertically toward the seabed. We found three key design features that improved the efficiency of the apparatus: (1) an open area of sea surface and mesh size of less than 100 μm produced high fertilization and optimal survival (>90%), (2) a special skirt‐shaped net (3 m in diameter) with a connection hose for attaching the cradle to collect bundles from many adult colonies over a wide area and at various depths, and (3) adding short square tube pieces, called square hollow sections, as a substrate for enhancing larval settlement and survival, to a larval cradle at 4 days after spawning was optimal for uniform settlement. This system allowed not only the collection of several million eggs, but also subsequent production of several thousand settled juvenile corals, without land facilities. Our design achieved several hundred times higher survival for early life stages of Acropora tenuis compared to nature. 相似文献
56.
Studies on DNA markers (D4S10 and D4S43/S127) genetically linked to Huntington's disease in Japanese families 总被引:1,自引:0,他引:1
Ichiro Kanazawa Ikuko Kondo Joh-E Ikeda Teruaki Ikeda Yuichior Shizu Mitsuo Yoshida Hirotaro Narabayashi Shigetoshi Kuroda Hisayuki Tsunoda Eiji Mizuta Yoko Okuno Kiyotaka Sugawara Miho Murata Mafuyu Takahashi James F. Gusella 《Human genetics》1990,85(3):257-260
Summary This is the first full report on the genetic linkage between Japanese Huntington's disease and the DNA markers D4S10 and D4S43/S127. With use of the HindIII, BglI, and EcoRI polymorphisms detected at D4S10, and the combination of all these polymorphisms to give composite haplotypes, nine Japanese Huntington's disease families were found to be informative. Three recombinants for D4S10 were detected in these families, giving a maximum lod score of 1.662 at a of 0.10. Similarly, when we used the MspI and PvuII polymorphisms detected by D4S43/S127, five families gave informative results. No recombinant was detected in these families, giving a maximum lod score of 3.348 at a of 0.00. These results clearly support the view that the Japanese Huntington's disease gene may be identical with the Western gene, in spite of the lower prevalence rate in Japan. 相似文献
57.
Reproductive compensation of a perennial plant Sanguisorba tenuifolia to herbivory by Phengaris teleius 下载免费PDF全文
Plants that are consumed by herbivores incur a reduction in fitness. Therefore, plants need to prepare to defend against and/or avoid herbivory using ‘resistance’ and/or ‘tolerance’ systems. Phengaris teleius is a specialist herbivore of Sanguisorba tenuifolia. Phengaris teleius lays eggs in the flower buds of S. tenuifolia and the larvae of P. teleius feed on the ovaries and ovules of S. tenuifolia. In order to clarify the extent of herbivory damage by P. teleius and effect on reproduction in S. tenuifolia, we conducted field observations and artificial cutting experiments carried out in the natural habitat where both P. teleius and S. tenuifolia live sympatrically. Phengaris teleius often lays one egg per spike, and about half of the total eggs were laid on the spike that developed at the shoot apex and was largest in size. The fruits were damaged most heavily in the spike in which an egg was laid. However, the number of fruits was almost the same between individuals with no damage and individuals with a feeding damage rate of 40% or less. This showed that compensation would be achieved by increasing the number of fruits in other spikes without damage. On the other hand, because no compensation was detected in the cutting experiments, it was considered that compensation may be induced by biological stimuli produced by P. teleius. These responses may be one of the mechanisms for continuing the interspecific relationship between S. tenuifolia (host plant) and P. teleius (herbivore insect). 相似文献
58.
Takashi Nobusawa Yoko Okushima Noriko Nagata Mikiko Kojima Hitoshi Sakakibara Masaaki Umeda 《PLoS biology》2013,11(4)
Plant organ growth is controlled by inter-cell-layer communication, which thus determines the overall size of the organism. The epidermal layer interfaces with the environment and participates in both driving and restricting growth via inter-cell-layer communication. However, it remains unknown whether the epidermis can send signals to internal tissue to limit cell proliferation in determinate growth. Very-long-chain fatty acids (VLCFAs) are synthesized in the epidermis and used in the formation of cuticular wax. Here we found that VLCFA synthesis in the epidermis is essential for proper development of Arabidopsis thaliana. Wild-type plants treated with a VLCFA synthesis inhibitor and pasticcino mutants with defects in VLCFA synthesis exhibited overproliferation of cells in the vasculature or in the rib zone of shoot apices. The decrease of VLCFA content increased the expression of IPT3, a key determinant of cytokinin biosynthesis in the vasculature, and, indeed, elevated cytokinin levels. These phenotypes were suppressed in ipt3;5;7 triple mutants, and also by vasculature-specific expression of cytokinin oxidase, which degrades active forms of cytokinin. Our results imply that VLCFA synthesis in the epidermis is required to suppress cytokinin biosynthesis in the vasculature, thus fine-tuning cell division activity in internal tissue, and therefore that shoot growth is controlled by the interaction between the surface (epidermis) and the axis (vasculature) of the plant body. 相似文献
59.
Ozaki TJ Sato N Kitajo K Someya Y Anami K Mizuhara H Ogawa S Yamaguchi Y 《Cognitive neurodynamics》2012,6(2):185-198
An ambiguous figure such as the Necker cube causes spontaneous perceptual switching (SPS). The mechanism of SPS in multistable
perception has not yet been determined. Although early psychological studies suggested that SPS may be caused by fatigue or
satiation of orientation, the neural mechanism of SPS is still unknown. Functional magnetic resonance imaging (fMRI) has shown
that the dorsal attention network (DAN), which mainly controls voluntary attention, is involved in bistable perception of
the Necker cube. To determine whether neural dynamics along the DAN cause SPS, we performed simultaneous electroencephalography
(EEG) and fMRI during an SPS task with the Necker cube, with every SPS reported by pressing a button. This EEG–fMRI integrated
analysis showed that (a) 3–4 Hz spectral EEG power modulation at fronto-central, parietal, and centro-parietal electrode sites
sequentially appeared from 750 to 350 ms prior to the button press; and (b) activations correlating with the EEG modulation
traveled along the DAN from the frontal to the parietal regions. These findings suggest that slow oscillation initiates SPS
through global dynamics along the attentional system such as the DAN. 相似文献
60.
Day SM Westfall MV Fomicheva EV Hoyer K Yasuda S La Cross NC D'Alecy LG Ingwall JS Metzger JM 《Nature medicine》2006,12(2):181-189
The myofilament protein troponin I (TnI) has a key isoform-dependent role in the development of contractile failure during acidosis and ischemia. Here we show that cardiac performance in vitro and in vivo is enhanced when a single histidine residue present in the fetal cardiac TnI isoform is substituted into the adult cardiac TnI isoform at codon 164. The most marked effects are observed under the acute challenges of acidosis, hypoxia, ischemia and ischemia-reperfusion, in chronic heart failure in transgenic mice and in myocytes from failing human hearts. In the isolated heart, histidine-modified TnI improves systolic and diastolic function and mitigates reperfusion-associated ventricular arrhythmias. Cardiac performance is markedly enhanced in transgenic hearts during reperfusion despite a high-energy phosphate content similar to that in nontransgenic hearts, providing evidence for greater energetic economy. This pH-sensitive 'histidine button' engineered in TnI produces a titratable molecular switch that 'senses' changes in the intracellular milieu of the cardiac myocyte and responds by preferentially augmenting acute and long-term function under pathophysiological conditions. Myofilament-based inotropy may represent a therapeutic avenue to improve myocardial performance in the ischemic and failing heart. 相似文献