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排序方式: 共有476条查询结果,搜索用时 15 毫秒
81.
82.
Koichi Izumikawa Yoichi Hirakata Toshiyuki Yamaguchi Ryoji Yoshida Michiko Nakano Junichi Matsuda Chikako Mochida Shigefumi Maesaki Kazunori Tomono Yasuaki Yamada Takayoshi Tashiro Shigeru Kohno Shimeru Kamihira 《Microbiology and immunology》1998,42(10):677-681
A total of 19 Escherichia coli O157 isolates were obtained in Nagasaki Prefecture, in the southwestern part of Japan, between 1990 and 1996. Pulsed-field gel electrophoresis (PFGE) and computer-assisted analysis were applied to determine genetic relationships among these strains. Fragment patterns of the isolates in Nagasaki, as determined by PFGE, were compared with those of isolates in other areas where large outbreaks and sporadic cases of E. coli O157 infection occurred. Similarity values of all the strains isolated in Nagasaki Prefecture were over 0.65 except for E. coli O26. Some strains were identical to the strains isolated from the areas where large outbreaks occurred. All strains were susceptible to ampicillin, fosfomycin, minocycline, amikacin, ofloxacin and sulfamethoxazole-trimethoprim. 相似文献
83.
84.
Makoto Sakurai Susumu Nakakoji Hisaya Manabe Shin-ichi Dewa Akihiko Shinomiya Tomoki Sunobe 《Ichthyological Research》2009,56(1):82-86
Bi-directional sex change in the deep-water gobiid fish Trimma yanagitai was examined. The gonads of all individuals consisted of ovarian and testicular elements, and an accessory gonadal structure.
In no gonads were both testicular and ovarian parts simultaneously active. Bi-directional sex changes occurred during the
rearing experiments in aquaria under conditions of which there was co-existence of two males or plural females. The sex of
individuals could be determined by their relative body size or social dominance: the largest individuals acting as male and
the remainder as female. 相似文献
85.
Sugawara A Torigoe T Tamura Y Kamiguchi K Nemoto K Oguro H Sato N 《Cell stress & chaperones》2009,14(2):133-139
Polyamine compound deoxyspergualin (DSG) is a potent immunosuppressive agent that has been applied clinically for protecting
graft rejection and treatment of Wegener's granulomatosis. Though DSG can bind to heat-shock proteins (HSPs) in cells, its
mechanism of immunosuppressive action remains unknown. It is widely accepted that extracellular HSPs are capable of stimulating
dendritic cells (DC) through cell surface receptors, leading to DC activation and cytokine release. In this study, we examined
if DSG analogs could inhibit HSP70-induced DC activation. Bone marrow derived immature mouse DCs and peripheral blood mononuclear
cell-derived immature human DCs were generated and incubated with Alexa 488-labeled Hsp70 in the presence of methoxyDSG (Gus-1)
that had comparable HSP70-binding affinity to DSG or DSG analog GUS-7, which had much more reduced binding affinity for HSP70.
The binding of HSP70 to immature DCs was analyzed by laser microscopy and flow cytometry. HSP70-induced DC activation was
assessed by TNF-α release by enzyme-linked immunosorbent assay. Binding of Hsp70 to the cell surface of immature DCs was inhibited
under the presence of Gus-1, but not under the presence of Gus-7. Immature DCs were activated and released TNF-α by the stimulation
with HSP70 for 12 hours; however, the HSP70-induced TNF-α release was suppressed under the presence of Gus-1, and partially
suppressed under the presence of Gus-7. Similar results were observed when immature human DCs were stimulated under the same
conditions. Immunosuppressive mechanism of DSG may be explained, at least in part, by the inhibition of extracellular HSP70-DC
interaction and HSP70-induced activation of immature DCs.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
86.
Wu H Ichikawa S Tani C Zhu B Tada M Shimoishi Y Murata Y Nakamura Y 《Biochimica et biophysica acta》2009,1791(1):8-16
Docosahexaenoic acid (22: 6n-3; DHA) is a long chain polyunsaturated fatty acid that exists highly enriched in fish oil, and it is one of the low molecular weight food chemicals which can pass a blood brain barrier. A preliminary survey of several fatty acids for expression of growth-associated protein-43 (GAP-43), a marker of axonal growth, identified DHA as one of the most potent inducers. The human neuroblastoma SH-SY5Y cells exposed to DHA showed significant and dose-dependent increases in the percentage of cells with longer neurites. To elucidate signaling mechanisms involved in DHA-enhanced basal neuritogenesis, we examined the role of extracellular signal-regulated kinase (ERK)1/2 and intracellular reactive oxygen species (ROS) production using SH-SY5Y cells. From immunoblotting experiments, we observed that DHA induced the ROS production, protein tyrosine phosphatase inhibition, mitogen-activated protein kinase (MAPK)/ERK kinase (MEK) phosphorylation, and sequentially ERK1/2 phosphorylation, the last of which was significantly reduced by MEK inhibitor U0126. Both antioxidants and MEK inhibitor affected DHA-induced GAP-43 expression, whereas the specific PI3K inhibitor LY294002 did not. We found that total protein tyrosine phosphatase activity was also downregulated by DHA treatment, which was counteracted by antioxidant pretreatment. These results suggest that the ROS-dependent ERK pathway, rather than PI3K, plays an important role during DHA-enhanced neurite outgrowth. 相似文献
87.
Yasuaki Tanaka Toshihiro Miyajima Yu Umezawa Takeshi Hayashibara Hiroshi Ogawa Isao Koike 《Journal of experimental marine biology and ecology》2009,377(2):101-106
The net production of dissolved organic matter (DOM) and dissolved combined and free amino acids (DCAA and DFAA, respectively) by the hermatypic coral Acropora pulchra was measured in the submerged condition, and the production rates were normalized to the coral surface area, tissue biomass, and net photosynthetic rates by zooxanthellae. When normalized to the unit surface area, the production rates of dissolved organic carbon and nitrogen (DOC and DON, respectively) were 37 and 4.4 nmol cm− 2 h− 1, respectively. Comparing with the photosynthetic rate by zooxanthellae, which was measured by 13C-tracer accumulation in the soft tissue of the coral colony, the release rate of DOC corresponded to 5.4% of the daily net photosynthetic production. The tissue biomass of the coral colony was 178 µmol C cm− 2 and 23 µmol N cm− 2, indicating that the release of DOC and DON accounted for 0.021% h− 1 and 0.019% h− 1 of the tissue C and N, respectively. The C:N ratios of the released DOM (average 8.4) were not significantly different from those of the soft tissue of the coral colonies (average 7.7). While DFAA did almost not accumulate in the incubated seawater, DCAA was considerably released by the coral colonies at the rate of 2.1 nmol cm− 2 h− 1 on average. Calculating C and N contents of the hydrolyzable DCAA, it was revealed that about 20% and 50%–60% of the released bulk DOC and DON, respectively, were composed of DCAA. 相似文献
88.
Shinji Ohsawa Takeshi Saito Shin Yoshikawa Hideo Mawatari Makoto Yamada Kazuhiro Amita Nobuki Takamatsu Yasuaki Sudo Tsuneomi Kagiyama 《Limnology》2010,11(3):207-215
One feature of volcanic lakes influenced by subaqueous fumaroles existing at lake bottoms (called active crater lakes) is
the remarkable color of their waters: turquoise or emerald green. The active crater lake named Yudamari at Mt. Nakadake of
Aso volcano, Japan, takes on a milky pale blue-green. The particular blue component of the lake water color results from Rayleigh
scattering of sunlight by very fine aqueous colloidal sulfur particles; the green component is attributable to absorption
of sunlight by dissolved ferrous ions. An objective color observation conducted during 2000–2007 revealed that the lake water
color changed from blue-green to solid green. The disappearance of the blue ingredient of the water color will result in diminution
of aqueous colloidal sulfur from chemical analyses of lake waters sampled simultaneously. The aqueous sulfur is produced by
the reaction of sulfur dioxide and hydrogen sulfide supplied from subaqueous fumaroles. However, its production efficiency
decreases by domination of sulfur dioxide in the subaqueous fumarolic sulfur gas species with increasing subaqueous fumarolic
temperature. The disappearance of blue ingredients from the blue-green color of the lake water may be attributed to activation
of subaqueous fumarole activity. 相似文献
89.
A novel alternative splicing isoform of human T-cell leukemia virus type 1 bZIP factor (HBZ-SI) targets distinct subnuclear localization 总被引:4,自引:0,他引:4
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