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61.
Zehua Zou Ruixuan Liu Yiwen Wang Yufei Xing Zuowei Shi Kaifu Wang Darning Dong 《Acta biochimica et biophysica Sinica》2021,(3):294-303
The occurrence and progress of osteoporosis(OP)are partially caused by impaired osteoblast differentiation.Interleukin-I receptor antagonist(IL1RN)is an immune ... 相似文献
62.
表观遗传学对于微生物的生命进程起着重要作用。由限制-修饰系统调控的DNA修饰参与微生物的免疫防御系统,无限制-修饰系统调控的DNA修饰通过调控基因表达影响表型。然而,表观遗传信息还没有被常规地作为DNA信息收集分析。基于对DNA合成反应的动力学分析,单分子实时测序技术可以在获得基本序列数据的同时实现对被修饰核苷酸的检测。这个技术为微生物中已知DNA修饰的研究提供了新的平台,也为新型DNA修饰的发现做好准备。本文综述了单分子实时测序技术及其在微生物表观遗传学中的应用。 相似文献
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Bo Pang Juanjuan Zhang Xi Zhang Jihong Yuan Yanan Shi Ling Qiao 《Acta biochimica et biophysica Sinica》2021,(3):354-364
Lipid metabolism reprogramming is now accepted as a new hallmark of cancer.Hence,target-ing the lipogenesis pathway may be a potential avenue for cancer treatme... 相似文献
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Programmed death‐ligand 1 (PD‐L1) is involved in immunosuppression in variety of tumours. Regulatory B cells (Bregs) are critical immune regulatory cells, and it has been demonstrated that the number of regulatory B cells in patients with acute myeloid leukaemia (AML) is much higher than that in healthy donors (HDs), which is linked to a poor prognosis. This study aimed to determine whether increased expression of PD‐L1, including in Bregs, is associated with a worse prognosis in individuals with AML. The proportion of Bregs, PD‐L1 expression in Bregs and PD‐1 expression in T cells were determined using flow cytometry using patient samples from 21 newly diagnosed AML patients at different stages of treatment and 25 HDs. We confirmed PD‐L1 expression in Bregs, and PD‐1 expression in CD3+CD4+T cells in bone marrow and peripheral blood samples from AML patients was higher than that in samples from HDs. The complete remission (CR) and progression‐free survival (PFS) of Bregs with high PD‐L1 expression were significantly decreased following induction chemotherapy. PD‐L1 expression is indeed increased in Bregs from individuals with AML, and high PD‐L1 expression is related to a poor prognosis. 相似文献
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流行性出血热地鼠肾细胞灭活疫苗人体试用细胞免疫反应观察 总被引:2,自引:0,他引:2
观察了20位志愿者在接种试验性流行性出血热(EHF)地鼠肾细胞(GHKC)双价灭活疫苗后的细胞免疫反应,并以其体液免疫反应作对照观察。用淋巴细胞转化试验测定细胞免疫水平。结果首针疫苗接种后42天和56天,特异性刺激指数(SSI)和非特异性刺激指数(NSI)均较免疫前显著增高(P_(SSI)<0.001;P_(NSI)<0.02),免疫后SSI累计阳转率为100%,NSI累计阳转率为60%,免疫后6个月二者均降低至正常水平。免疫后56天测定抗体,荧光抗体阳转率为100%;微量感染性中和试验表明,针对家鼠型病毒L99株中和抗体阳转率为95%,而针对野鼠型病毒JR株阳转率为65%。 相似文献
69.
Since the first human case of H5N1 avian influenza virus infection was reported in 1997, this highly pathogenic virus has infected hundreds of people around the world and resulted in many deaths. The ability of H5N1 to cross species boundaries, and the presence of polymorphisms that enhance virulence, present challenges to developing clear strategies to prevent the pandemic spread of this highly pathogenic avian influenza (HPAI) virus. This review summarizes the current understanding of, and recent research on, the avian influenza H5N1 virus, including transmission, virulence, pathogenesis, clinical characteristics, treatment and prevention. 相似文献
70.
Zhiyi Liu Zilu Tian Kuan Cao Bin Zhang Quan Wen Xinyu Zhou Weibin Yang Tao Wang Hengliang Shi Renhao Wang 《Journal of cellular and molecular medicine》2019,23(1):70-82
The tumour susceptibility gene 101 (TSG101) is reported to play important roles in the development and progression of several human cancers. However, its potential roles and underlined mechanisms in human hepatocellular carcinoma (HCC) are still needed to be further clarified. In the present study, we reported that knock down of TSG101 suppressed the proliferation, migration and invasion of HCC cells, while overexpression of TSG101 facilitated them. Molecularly, the results revealed that knock down of TSG101 significantly decreased the cell cycle related regulatory factor p53 and p21. In another point, knock down of TSG101 also obviously decreased the level of metallopeptidase inhibitor TIMP1 (Tissue inhibitors of metalloproteinases 1), which results in inhibition of MMP2, MMP7 and MMP9. In contrast, overexpression of TSG101 had opposite effects. The iTRAQ proteomics analysis identified that oncogenic protein PEG10 (Paternally expressed gene 10) might be a potential downstream target of TSG101. Further investigation showed that TSG101 interacted with PEG10 and protected it from proteasomal degradation thereby regulating the expression of p53, p21 and MMPs. Finally, we found that both TSG101 and PEG10 proteins are up‐regulated and presented a direct correlation in HCC patients. In conclusion, these results suggest that TSG101 is up‐regulated in human HCC patients, which may accelerate the proliferation, migration and invasion of HCC cells through regulating PEG10. 相似文献