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111.
New Insights into the Enterococcus faecalis CroRS Two-Component System Obtained Using a Differential-Display Random Arbitrarily Primed PCR Approach 下载免费PDF全文
Using a modified random arbitrarily primed PCR approach, the operon encoding the Enterococcus faecalis JH2-2 CroRS two-component regulatory system was shown to be repressed during stationary phase, and a CroRS-regulated operon (glnQHMP) was identified. Gel retardation assays showed that the CroR regulator binds specifically to the glnQHMP promoter. 相似文献
112.
Le Breton Y Muller C Auffray Y Rincé A 《Applied and environmental microbiology》2007,73(11):3738-3741
Using a modified random arbitrarily primed PCR approach, the operon encoding the Enterococcus faecalis JH2-2 CroRS two-component regulatory system was shown to be repressed during stationary phase, and a CroRS-regulated operon (glnQHMP) was identified. Gel retardation assays showed that the CroR regulator binds specifically to the glnQHMP promoter. 相似文献
113.
Organization of a functional chicken class II B gene 总被引:8,自引:4,他引:8
114.
H. R. Juul-Madsen Rima Zoorob Charles Auffray Karsten Skjødt Jan E. Hedemand 《Immunogenetics》1997,45(6):345-352
New chicken Rfp-Y haplotypes were determined by the use of restriction fragment length polymorphism (RFLP) and mixed lymphocyte culture (MLC) in four different chicken haplotypes, B 15 , B 19 , B 21 , B 201 . The RFLP polymorphism was mapped to the Rfp-Y system by the use of a subclone (18.1) which maps near a polymorphic lectin gene located in the Rfp-Y system and DNA from families with known segregation of the implicated RFLP polymorphism. For the first time it is shown that major histocompatibility complex class II genes in the Rfp-Y system have functional implications. Sequence information of the B1 domain of the proposed Rfp-Y haplotypes was obtained which supported the functional data. 相似文献
115.
Britton-Davidian J Catalan J da Graça Ramalhinho M Auffray JC Claudia Nunes A Gazave E Searle JB da Luz Mathias M 《Genetical research》2005,86(3):171-183
The ancestral karyotype of the house mouse (Mus musculus) consists of 40 acrocentric chromosomes, but numerous races exist within the domesticus subspecies characterized by different metacentric chromosomes formed by the joining at the centromere of two acrocentrics. An exemplary case is present on the island of Madeira where six highly divergent chromosomal races have accumulated different combinations of 20 metacentrics in 500-1000 years. Chromosomal cladistic phylogenies were performed to test the relative performance of Robertsonian (Rb) fusions, Rb fissions and whole-arm reciprocal translocations (WARTs) in resolving relationships between the chromosomal races. The different trees yielded roughly similar topologies, but varied in the number of steps and branch support. The analyses using Rb fusions/fissions as characters resulted in poorly supported trees requiring six to eight homoplasious events. Allowance for WARTs considerably increased nodal support and yielded the most parsimonious trees since homoplasy was reduced to a single event. The WART-based trees required five to nine WARTs and 12 to 16 Rb fusions. These analyses provide support for the role of WARTs in generating the extensive chromosomal diversification observed in house mice. The repeated occurrence of Rb fusions and WARTs highlights the contribution of centromere-related rearrangements to accelerated rates of chromosomal change in the house mouse. 相似文献
116.
Dr. Yanick Auffray Xavier Gansel Bouachanh Thammavongs Philippe Boutibonnes 《Current microbiology》1992,24(5):281-284
Heat shock inLactococcus lactis subsp.lactis may induce as many as 16 proteins after a temperature shift from 30° to 40°C. Five induced proteins were found to be immunologically related to theEscherichia coli GroEL, DnaK, DnaJ, and GrpE proteins, and to theBacillus subtilis
43 factor. From these initial studies we conclude that, inL. lactis subsp.lactis, a heat shock response similar to that known to occur in other prokaryotes might exist. 相似文献
117.
Demeule M Régina A Annabi B Bertrand Y Bojanowski MW Béliveau R 《Molecular neurobiology》2004,30(2):157-183
The blood-brain barrier contributes to brain homeostasis by controlling the access of nutrients and toxic substances to the
central nervous system (CNS). The acquired brain endothelial cells phenotype results from their sustained interactions with
their microenvironment. The endothelial component is involved in the development and progression of most CNS diseases such
as brain tumors, Alzheimer’s disease, or stroke, for which efficient treatments remain to be discovered. The endothelium constitutes
an attractive therapeutical target, particularly in the case of brain tumors, because of the high level of angiogenesis associated
with this disease. Drug development based on targeting differential protein expression in the vasculature associated with
normal tissues or with disease states holds great potential. This article highlights some of the growing body of evidence
showing molecular differences between the vascular bed phenotype of normal and pathological endothelium, with a particular
focus on brain tumor endothelium targets, which may play crucial roles in the development of brain cancers. Finally, an overview
is presented of the emerging therapies for brain tumors that take the endothelial component into consideration.
Equal first authors 相似文献
118.
Banka S Blom HJ Walter J Aziz M Urquhart J Clouthier CM Rice GI de Brouwer AP Hilton E Vassallo G Will A Smith DE Smulders YM Wevers RA Steinfeld R Heales S Crow YJ Pelletier JN Jones S Newman WG 《American journal of human genetics》2011,(2):214-225
Dihydrofolate reductase (DHFR) is a critical enzyme in folate metabolism and an important target of antineoplastic, antimicrobial, and antiinflammatory drugs. We describe three individuals from two families with a recessive inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency due to a germline missense mutation in DHFR, resulting in profound enzyme deficiency. We show that cerebral folate levels, anemia, and pancytopenia of DHFR deficiency can be corrected by treatment with folinic acid. The characterization of this disorder provides evidence for the link between DHFR and metabolism of cerebral tetrahydrobiopterin, which is required for the formation of dopamine, serotonin, and norepinephrine and for the hydroxylation of aromatic amino acids. Moreover, this relationship provides insight into the role of folates in neurological conditions, including depression, Alzheimer disease, and Parkinson disease. 相似文献
119.
Involvement of the low-density lipoprotein receptor-related protein in the transcytosis of the brain delivery vector angiopep-2 总被引:1,自引:0,他引:1
Demeule M Currie JC Bertrand Y Ché C Nguyen T Régina A Gabathuler R Castaigne JP Béliveau R 《Journal of neurochemistry》2008,106(4):1534-1544
The blood–brain barrier (BBB) restricts the entry of proteins as well as potential drugs to cerebral tissues. We previously reported that a family of Kunitz domain-derived peptides called Angiopeps can be used as a drug delivery system for the brain. Here, we further characterize the transcytosis ability of these peptides using an in vitro model of the BBB and in situ brain perfusion. These peptides, and in particular Angiopep-2, exhibited higher transcytosis capacity and parenchymal accumulation than do transferrin, lactoferrin, and avidin. Angiopep-2 transport and accumulation in brain endothelial cells were unaffected by the P-glycoprotein inhibitor, cyclosporin A, indicating that this peptide is not a substrate for the efflux pump P-glycoprotein. However, competition studies show that activated α2 -macroglobulin, a specific ligand for the low-density lipoprotein receptor-related protein-1 (LRP1) and Angiopep-2 can share the same receptor. In addition, LRP1 was detected in glioblastomas and brain metastases from lung and skin cancers. Fluorescent microscopy also revealed that Alexa488-Angiopep-2 co-localized with LRP1 in brain endothelial cell monolayers. Overall, these results suggest that Angiopep-2 transport across the BBB is, in part, mediated by LRP1. 相似文献
120.
Drug specific resistance to oxaliplatin is associated with apoptosis defect in a cellular model of colon carcinoma 总被引:1,自引:0,他引:1
Gourdier I Del Rio M Crabbé L Candeil L Copois V Ychou M Auffray C Martineau P Mechti N Pommier Y Pau B 《FEBS letters》2002,529(2-3):232-236
To investigate acquired resistance to oxaliplatin, we selected two resistant clones from the HCT116 cell line. We found that the resistant phenotype was associated with resistance to oxaliplatin-induced apoptosis as demonstrated by FACS analysis and by Western blotting of caspase 3 activation. In addition, the resistant phenotype showed a concomitant resistance to lonidamine and arsenic trioxide which are inducers of mitochondrial apoptosis. Furthermore, a complete loss of Bax expression due to a frameshift mutation was observed in the most resistant clone. Taken together, these findings suggest that altered mitochondrial-mediated apoptosis could play a role in oxaliplatin resistance. 相似文献