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991.
Xingyi Ge Yongquan Wu Meiniang Wang Jun Wang Lijun Wu Xinglou Yang Yuji Zhang Zhengli Shi 《中国病毒学》2013,28(5):280-290
East Lake (Lake Donghu), located in Wuhan, China, is a typical city freshwater lake that has been experiencing eutrophic conditions and algal blooming during recent years. Marine and fresh water are considered to contain a large number of viruses. However, little is known about their genetic diversity because of the limited techniques for culturing viruses. In this study, we conducted a viral metagenomic analysis using a high-throughput sequencing technique with samples collected from East Lake in Spring, Summer, Autumn, and Winter. The libraries from four samples each generated 234,669, 71,837, 12,820, and 34,236 contigs (> 90 bp each), respectively. The genetic structure of the viral community revealed a high genetic diversity covering 23 viral families, with the majority of contigs homologous to DNA viruses, including members of Myoviridae, Podoviridae, Siphoviridae, Phycodnaviridae, and Microviridae, which infect bacteria or algae, and members of Circoviridae, which infect invertebrates and vertebrates. The highest viral genetic diversity occurred in samples collected in August, then December and June, and the least diversity in March. Most contigs have low-sequence identities with known viruses. PCR detection targeting the conserved sequences of genes (g20, psbA, psbD, and DNApol) of cyanophages further confirmed that there are novel cyanophages in the East Lake. Our viral metagenomic data provide the first preliminary understanding of the virome in one freshwater lake in China and would be helpful for novel virus discovery and the control of algal blooming in the future. 相似文献
992.
同地共栖三种鼠耳蝠食性差异及其生态位分化 总被引:1,自引:0,他引:1
2005年9—11月在贵州省安龙县笃山乡暗河村,分析了共栖同一山洞3种鼠耳蝠的形态特征和食性。在体型上,华南水鼠耳蝠体重为(4.46±0.53)g,前臂长为(34.63±1.45)mm;毛腿鼠耳蝠体重为(5.15±1.76)g,前臂长为(35.20±1.07)mm;西南鼠耳蝠体重为(10.94±0.87)g,前臂长为(45.21±1.15)mm。3种鼠耳蝠的体重两两之间差异显著,西南鼠耳蝠与另外2种鼠耳蝠的前臂长相比两两之间差异显著。在食物组成上,华南水鼠耳蝠主要捕食近水面活动的双翅目及其幼虫,体积百分比和频次百分比分别为79.7%和100%;毛腿鼠耳蝠主要捕食双翅目和小型鞘翅目,体积百分比分别占59.6%和28.8%,频次百分比分别为91.3%和80.1%;西南鼠耳蝠的食物组成主要为近地面或在地表活动的鞘翅目步甲科和埋葬虫科昆虫,体积百分比和频次百分比分别为80.8%和100%;3种鼠耳蝠食物组成存在显著差异。结果表明,同地共栖3种鼠耳蝠除了形态结构上出现差异,食物组成也存在明显的差异。据此,推测3种鼠耳蝠可能采取不同的捕食生境和捕食策略,从而导致捕食生态位分离,避免出现激烈竞争,使得3种近缘鼠耳蝠能够同地共栖。 相似文献
993.
Xi Jiang Jingyin Yue Huimei Lu Neil Campbell Qifeng Yang Shijie Lan Bruce G. Haffty Changji Yuan Zhiyuan Shen 《International journal of biological sciences》2013,9(1):67-77
Filamin-A cross-links actin filaments into dynamic orthogonal networks, and interacts with an array of proteins of diverse cellular functions. Because several filamin-A interaction partners are implicated in signaling of cell mobility regulation, we tested the hypothesis that filamin-A plays a role in cancer metastasis. Using four pairs of filamin-A proficient and deficient isogenic cell lines, we found that filamin-A deficiency in cancer cells significantly reduces their migration and invasion. Using a xenograft tumor model with subcutaneous and intracardiac injections of tumor cells, we found that the filamin-A deficiency causes significant reduction of lung, splenic and systemic metastasis in nude mice. We evaluated the expression of filamin-A in breast cancer tissues by immunohistochemical staining, and found that low levels of filamin-A expression in cancer cells of the tumor tissues are associated with a better distant metastasis-free survival than those with normal levels of filamin-A. These data not only validate filamin-A as a prognostic marker for cancer metastasis, but also suggest that inhibition of filamin-A in cancer cells may reduce metastasis and that filamin-A can be used as a therapeutic target for filamin-A positive cancer. 相似文献
994.
甜菜碱醛脱氢酶(BADH)在植物抗逆反应中发挥着重要作用。文中从胡杨cDNA克隆到2个甜菜碱醛脱氢酶基因,分别命名为PeBADH1和PeBADH2。PeBADH1和PeBADH2均编码503个氨基酸的蛋白质,预测分子量分别是54.93 kDa和54.90 kDa。组织表达模式分析发现这2个基因在正常生长、盐和H2O2胁迫下,在不同组织中的表达模式有较大差异。在大肠杆菌中表达并纯化了2个基因的重组蛋白。酶活性分析显示PeBADH1和PeBADH2蛋白对底物的活性分别是0.073μmol/(min.mg)和0.107μmol/(min.mg)。热力学稳定性分析显示这2个蛋白的热力学稳定性具有明显差异。因此,基因表达模式差异与蛋白质酶学性质的不同预示着这2个基因可能存在功能上的分化。 相似文献
995.
克雷伯氏菌(Klebsiella pneumonia)甘油歧化发酵生产1,3-丙二醇(1,3-PD)的过程中,乳酸是氧化途径最主要的副产物,乳酸的产生和积累,不仅限制了菌体本身的生长,而且严重影响了1,3-丙二醇的转化率。利用λRed重组技术对Klebsiella pneumonia中的酶乳酸脱氢酶基因(ldhA)进行改造。在λRed重组系统作用下,将带有300 bp的线性同源片段ldhA1-Cm-ldh A2与基因组DNA的同源重组,经过抗性筛选和PCR鉴定最终获得了ldhA基因缺失菌株K.pneumonia2-1ΔldhA。经过24 h发酵可知,乳酸最大产出浓度由原来的10.16 g/L降为0.49 g/L,1,3-PD由原来的78.83 g/L增长为85.76 g/L,甘油转化率由60.64%增长到65.97%,提高了5.33%。 相似文献
996.
997.
八肽胆囊收缩素对内毒素休克时海马损伤的影响及其机制初探 总被引:2,自引:0,他引:2
目的:观察八肽胆囊收缩素(CCK-8)对内毒素休克(ES)时海马损伤的影响,并探讨其可能的作用机制。方法:将日本大耳白兔经静脉注入内毒素的主要活性成分脂多糖(LPS,8mg/kg)复制ES模型。动物(32只)随机分为对照组、LPS组、CCK-8+LPS组和非特异性CCK受体拮抗剂丙谷胺(Pro)+LPS组(n=8)。监测平均动脉压(MAP)的变化,光、电镜观察海马的组织形态学改变,比色法检测海马NOS和SOD活性、N0和MDA含量的改变.用SD大鼠(12只,同上复制模型及分组)以免疫组织化学染色法观察海马iNOS和nNOS表达的变化。结果:与对照组相比,注入LPS后出现MAP显著而持续下降(P〈0.01);海马部位神经元损伤明显;iNOS和nNOS表达增强,NOS活性、NO和MDA含量显著升高(P〈0.05、P〈0.01和P〈0.01),SOD活性则降低(P〈0.01)。预先注入CCK-8可明显减轻上述变化,预先注入Pro则加剧以上变化。结论:CCK-8可减轻ES时脑内海马部位的损伤。其机制可能与其抗氧化作用和抑制NO的过量生成有关。 相似文献
998.
Bing Xu Shaolin Wang Yan Jiang Linlin Yang Ping Li Caixia Xie 《Animal biotechnology》2013,24(4):217-225
Grass carp, Ctenopharyngodon idellus (Valenciennes, 1844), is an economically important species widely cultured in the world, but its genome research resources are largely lacking. The objectives of this study were to construct normalized cDNA libraries for efficient EST analysis, to generate ESTs from these libraries, and to identify EST-related molecular markers such as microsatellites and single nucleotide polymorphisms (SNPs) for genetic analysis of this species. A total of 6,269 ESTs were generated representing 4,815 unique sequences, from which 105 putative microsatellites and 5,228 SNPs were identified. These genome resources provide the material basis for future genetic and functional analyses in this species. 相似文献
999.
1000.
The objective of this study was to analyze the clinical manifestation, imaging characteristics, genotype, and the relationship
between the three aforementioned parameters in two pedigrees suffering from spinocerebellar ataxia. To evaluate the clinical
manifestation of the two pedigrees and to compare the characteristics, we performed the MRI analysis of some patients from
both pedigrees, while 2 ml of the peripheral blood sample was collected for gene analysis. The gene analysis data showed that
pedigree 1 was certified spinocerebellar ataxia type-2 (SCA2); the CAG repeats in the proband, proband’s mother, and proband’s
brother were 44, 36, and 38, respectively. The MRI revealed brainstem cerebellar atrophy and “cross sign” and “ordinate sign”
of pons. Pedigree 2 was certified SCA1; the CAG repeats of the proband, proband’s aunt, and proband’s asymptomatic cousin
were 60, 51, and 52, respectively. The MRI revealed cerebellar atrophy in these individuals. We, therefore, concluded that
it was difficult to diagnose the SCA subset solely through the clinical manifestation. The imaging characteristics analysis
and final diagnosis depended basically on gene analysis data. 相似文献