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101.
Protein enrichment of sweet potato residue with co-culture of amylolytic fungi by solid-state fermentation 总被引:1,自引:0,他引:1
Yang SS 《Biotechnology advances》1993,11(3):495-505
Protein enrichment of sweet potato residue with amylolytic moulds by solid-state fermentation was higher than that obtained with amylolytic yeasts. The optimum initial moisture content for protein enrichment was 66% to 75%. Incrementally added nitrogen sources to the culture at zero time and at 24 h considerably improved the final protein content. During the cultivation, the moisture, ash and ATP contents increased, while the pH value decreased. A 1:1 co-culture of amylolytic mycelial fungi yielded a product with 32.4% crude protein after 4 days incubation at 30 degrees C. 相似文献
102.
pKa1 values of 3-methoxy-N-desmethyldiazepam in acetonitrile and methanol containing various acid concentrations were determined by spectrophotometry to be 3.5 and 1.3, respectively. Temperature-dependent racemization of enantiomeric 3-methoxy-N-desmethyldiazepam in methanol containing 0.5 M H2SO4 was studied by circular dichroism spectropolorimetry and the racemization reactions were found to follow apparent first-order kinetics. Thermodynamic parameters of the racemization reaction were found to be: Eact = 18.8 kcal/mol, and at 25°C: ΔH? = 18.3 kcal/mol, ΔS? = ?14.8 entropy unit, and ΔG? = 22.7 kcal/mol, respectively. The racemization had an isotope effect (kH/kD) of 1.6 at 42°C. Based on the results of this report and those of earlier reports by other investigators, a nucleophilically solvated C3 carbocation intermediate resulting from either a P (plus) or an M (minus) conformation is proposed to be an intermediate and responsible for the stereoselective nucleophilic substitution and the subsequent racemization of 3-methoxy-N-desmethyldiazepam enantiomers. © 1993 Wiley-Liss, Inc. 相似文献
103.
Wang Zunxin Wang Xianyu Liu Siqin Yang Ying Li Yang Chen Siyuan Wang Guangpeng Zhang Xincheng Ye Yuxiu Hu Laibao Zhou Qing Wang Feibing Chen Xinhong 《Journal of Plant Growth Regulation》2023,42(1):294-303
Journal of Plant Growth Regulation - Zinc is an important micronutrient for the growth and development of human body and plants. Proper use of nitrogen fertilizer and foliar application of Zn have... 相似文献
104.
105.
Xiong Zhang Ming-You Peng En-Ming Feng Qing-Dan Li Lu Chen Hu-Cheng Yang Bing Guo Hong Liang Ying-Tong Di Lei Tang Ying Yan 《化学与生物多样性》2023,20(10):e202301061
Three previously undescribed diterpenoids, helioscopnoids A–C, and eight known compounds were isolated from the whole plants of Euphorbia helioscopia. Their structures were established by extensive analysis of spectra and data comparison with previous literatures. Among them, compound 4 was identified as 24,24-dimethoxy-25,26,27-trinoreuphan-3β-ol with revised configurations of C-13, C-14, and C-17 (13R*, 14R*, 17R*). Cytotoxicity assays revealed that all compounds exhibited varying levels of cytotoxicity against H1975 cells, with compound 9 displaying the most potent activity, as indicated by cell viability rates of 18.13 % and 20.76 % at concentrations of 20 μM and 5 μM, respectively. This study expands the understanding of E. helioscopia terpenoids’ structural diversity and biological activities, contributing to the exploration of potential therapeutic applications. 相似文献
106.
Le Zhang Yi-Wei Huang Jia-Lin Huang Ji-Dong Ya Meng-Qing Zhe Chun-Xia Zeng Zhi-Rong Zhang Shi-Bao Zhang De-Zhu Li Hong-Tao Li Jun-Bo Yang 《Molecular ecology resources》2023,23(2):424-439
Cymbidium is an orchid genus that has undergone rapid radiation and has high ornamental, economic, ecological and cultural importance, but its classification based on morphology is controversial. The plastid genome (plastome), as an extension of plant standard DNA barcodes, has been widely used as a potential molecular marker for identifying recently diverged species or complicated plant groups. In this study, we newly generated 237 plastomes of 50 species (at least two individuals per species) by genome skimming, covering 71.4% of members of the genus Cymbidium. Sequence-based analyses (barcoding gaps and automatic barcode gap discovery) and tree-based analyses (maximum likelihood, Bayesian inference and multirate Poisson tree processes model) were conducted for species identification of Cymbidium. Our work provides a comprehensive DNA barcode reference library for Cymbidium species identification. The results show that compared with standard DNA barcodes (rbcL + matK) as well as the plastid trnH-psbA, the species identification rate of the plastome increased moderately from 58% to 68%. At the same time, we propose an optimized identification strategy for Cymbidium species. The plastome cannot completely resolve the species identification of Cymbidium, the main reasons being incomplete lineage sorting, artificial cultivation, natural hybridization and chloroplast capture. To further explore the potential use of nuclear data in identifying species, the Skmer method was adopted and the identification rate increased to 72%. It appears that nuclear genome data have a vital role in species identification and are expected to be used as next-generation nuclear barcodes. 相似文献
107.
108.
Summary
Chlorella vulgaris UTEX259 was cultivated using two different methods of gas supply. In one method the CO2 concentration in bubbled gas was held constant and in the other method it was increased gradually. Algal growth was almost linear after a short period of lag phase in both methods. With the constant CO2 concentration, the CO2 fixation rate in the linear growth phase decreased over 10%(v/v) CO2, while the rate increased up to 6% CO2. However, the rate was enhanced by using the latter incremental increase method, especially under a higher concentration of CO2. The maximum rate of CO2 fixation was 52 mg CO2/l·h at 20% CO2 during the gradual increase of CO2 concentration. 相似文献
109.
110.
原位缺口平移标记断裂DNA技术及其在检测流行性出血热尸检和实验动物组织中细胞凋亡和早期死亡的应用 总被引:1,自引:0,他引:1
原位缺口平移技术(ISNT)已被用于检测细胞核中DNA断裂鉴别尸检组织中细胞的凋亡和坏死断裂、流行性出血热(EHF)组织中存在散在单个细胞变性死亡和灶性梗死样坏死,前者带有细胞凋亡的特征。本文以EHF肝脏和实验性病毒感染鼠脑组织为例,应用缺口平移法,在DNA聚合酶或Klenow酶的作用下,将地高辛标记的dUTP掺入合成到DNA的断裂部位,通过碱性磷酸酶标抗地高辛抗体免疫组化法显示细胞DNA的断裂,检测和鉴别细胞的凋亡和坏死。为分析死后解剖时间间隔及组织固定时间对该方法的影响,本文选用死后2~140h尸检、经常规固定石蜡包埋后存放10~35年的标本和在10%福尔马林固定了10~35年之后再进行常规处理的标本。实验时用蛋白酶K(PK)消化前后对比并分别在标记反应液中略去DNA聚合酶作为阴性对照,用DNA酶消化组织人为制造DNA缺日作为阳性对照。结果发现,未经PK消化的组织,仅灶性肝细胞核ISNT标记阳性,经PK消化后,散在的带有凋亡特征的肝细胞胞核也出现阳性,灶性肝细胞胞核标记染色增强,但无论是蛋白酶消化与否,明确梗死样坏死的肝细胞均不被标记。结果还发现,死后2~24h内尸检组织和长时间(10~34年)存放的石 相似文献