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81.
Guiling Ma Yanfang Li Juan Zhang Hao Liu Dongyan Hou Lei Zhu Zhenyu Zhang Lin Zhang 《PloS one》2013,8(3)
Background
Pre-eclampsia is the leading cause of maternal and neonatal morbidity and mortality with incompletely understood etiopathogenesis. The purpose of the current study is to determine whether there is a relationship between the presence of autoantibodies against β1, β2 and α1 adrenoreceptors and severe pre-eclampsia.Methodology/Principal Findings
Synthetic peptides corresponding to amino acid sequences of the second extracellular loops of β1, β2 and α1 adrenoreceptors were synthesized as antigens to test 34 patients with severe pre-eclampsia, 36 normal pregnancy women and 40 non-pregnant controls for the presence of autoantibodies using enzyme-linked immunosorbent assay. The respective frequencies of autoantibodies against β1, β2 and α1 adrenoreceptors were 50.0% (17/34), 52.9% (18/34) and 55.9% (19/34) in patients with severe pre-eclampsia, 19.4% (7/36) (p = 0.011), 19.4% (7/36) (p = 0.006) and 17.6% (6/36) (p = 0.001) in normal pregnancy women and 10% (4/40), 7.5% (3/40) and 10% (4/40) (p<0.001) in non-pregnant controls. Titers of these autoantibodies were also significantly increased in patients with severe pre-eclampsia. By logistic regression analysis, the presence of these three autoantibodies significantly increased the risk of neonatal death (odds ratio, 13.5; 95% confidence interval, 1.3–141.3; p = 0.030) and long-term neonatal hospitalization (odds ratio, 5.0; 95% confidence interval, 1.3–19.1; p = 0.018). The risk of hypertension and fetal distress were also associated with the presence of these three autoantibodies.Conclusions/Significance
This novel pilot study demonstrated for the first time that the presence of autoantibodies against β1, β2 and α1 adrenoreceptors are increased in patients with severe pre-eclampsia. Pregnant women who are positive for the three autoantibodies are at increased risks of neonatal mortality and morbidity. We posit that these autoantibodies may be involved in the pathogenesis of severe pre-eclampsia. 相似文献82.
Zhao Shan Qinglin Han Jia Nie Xuezhi Cao Zuojia Chen Shuying Yin Yayi Gao Fang Lin Xiaohui Zhou Ke Xu Huimin Fan Zhikang Qian Bing Sun Jin Zhong Bin Li Andy Tsun 《The Journal of biological chemistry》2013,288(49):35093-35103
Although lysine methylation is classically known to regulate histone function, its role in modulating antiviral restriction factor activity remains uncharacterized. Interferon-induced transmembrane protein 3 (IFITM3) was found monomethylated on its lysine 88 residue (IFITM3-K88me1) to reduce its antiviral activity, mediated by the lysine methyltransferase SET7. Vesicular stomatitis virus and influenza A virus infection increased IFITM3-K88me1 levels by promoting the interaction between IFITM3 and SET7, suggesting that this pathway could be hijacked to support infection; conversely, IFN-α reduced IFITM3-K88me1 levels. These findings may have important implications in the design of therapeutics targeting protein methylation against infectious diseases. 相似文献
83.
Svetlana A. Romanenko Polina L. Perelman Natalya A. Serdukova Vladimir A. Trifonov Larisa S. Biltueva Jinhuan Wang Tangliang Li Wenhui Nie Patricia C.M. O’Brien Vitaly T. Volobouev Roscoe Stanyon Malcolm A. Ferguson-Smith Fengtang Yang Alexander S. Graphodatsky 《Mammalian genome》2006,17(12):1183-1192
The laboratory mouse (Mus musculus, 2n = 40), the Chinese hamster (Cricetulus griseus, 2n = 22), and the golden (Syrian) hamster (Mesocricetus auratus, 2n = 44) are common laboratory animals, extensively used in biomedical research. In contrast with the mouse genome, which was
sequenced and well characterized, the hamster species has been set aside. We constructed a chromosome paint set for the golden
hamster, which for the first time allowed us to perform multidirectional chromosome painting between the golden hamster and
the mouse and between the two species of hamster. From these data we constructed a detailed comparative chromosome map of
the laboratory mouse and the two hamster species. The golden hamster painting probes revealed 25 autosomal segments in the
Chinese hamster and 43 in the mouse. Using the Chinese hamster probes, 23 conserved segments were found in the golden hamster
karyotype. The mouse probes revealed 42 conserved autosomal segments in the golden hamster karyotype. The two largest chromosomes
of the Chinese hamster (1 and 2) are homologous to seven and five chromosomes of the golden hamster, respectively. The golden
hamster karyotype can be transformed into the Chinese hamster karyotype by 15 fusions and 3 fissions. Previous reconstructions
of the ancestral murid karyotype proposed diploid numbers from 2n = 52 to 2n = 54. By integrating the new multidirectional chromosome painting data presented here with previous comparative genomics
data, we can propose that syntenies to mouse Chrs 6 and 16 were both present and to hypothesize a diploid number of 2n = 48 for the ancestral Murinae/Cricetinae karyotype. 相似文献
84.
Ying Yang Zhenlong Wu Yue Chen Jian Qiao Mingyu Gao Jianmin Yuan Wei Nie Yuming Guo 《Biometals》2006,19(1):71-81
Magnesium deficiency and oxidative stress have been identified as correlative factors in many diseases. The origin of free
radicals correlated with oxidative damage resulting from Mg-deficiency is unclear at the cellular level. To investigate whether
hydrogen peroxide (H2O2) is associated in the oxidative stress induced by Mg-deficiency, the effect of Mg2+ deficiency (0, 0.4, 0.7 mM) on the metabolism of H2O2 was investigated in cultured chick embryo hepatocytes. After being cultured in the media with various concentrations of Mg2+ for 1, 2, 4, 6 and 10 days, parameters of H2O2 production, catalase activity, lipid peroxidation, intracellular total Mg and cell viability were analyzed. Results demonstrated
that long-term incubation of chick embryo hepatocyte in extracellular Mg2+-deprivative and Mg2+-deficient (0.4 mM) states significantly enhanced the production of H2O2 (approximately twofold, respectively) and lipid peroxidation in the cell cultures, while decreasing the cell viability. Additionally,
the reversing action of Mg2+ re-added to 1.0 mM and the partial reversing action of dimethylthiourea suggested that (i) [Mg2+]e deficiency induced the increase of H2O2 production, (ii) [Mg2+]e deficiency decreased catalase activity in chick embryo hepatocyte in vitro, subsequently causing oxidative stress and cell peroxidative damage. 相似文献
85.
Li Liu Rong Zhong Sheng Wei Hao Xiang Jigui Chen Duoshuang Xie Jieyun Yin Li Zou Jingwen Sun Wei Chen Xiaoping Miao Shaofa Nie 《PloS one》2013,8(4)
Background
Pathologic condition associated with metabolic syndrome traits seems to increase the risk of colorectal cancer. One mechanism underlying this relationship may involve the growth-promoting effects of the circulation hormones associated with obesity and insulin resistance, such as leptin.Methodology/Principal Findings
A two-stage case-control study was used to explore the role of polymorphisms of Leptin (LEP) and Leptin receptor (LEPR), either alone or in combination with environmental factors in colorectal carcinogenesis. In stage 1, 20 single nucleotide polymorphisms (SNPs) that tag common SNPs in these two genes were genotyped among 470 cases and 458 controls. In stage 2, another population with 314 cases and 355 controls were genotyped for the two most promising SNPs from stage 1. LEPR rs12037879 only presented modestly increased colorectal cancer risk, with odds ratios of 1.41 (95% confidence interval [CI] 1.13–1.76) and 1.74 (95%CI 1.08–2.81) for GA and AA genotype when compared with GG genotype in combined population. Smokers carrying LEPR rs12037879 A allele presented 1.67-fold (95%CI 1.39-fold to 2.01-fold) increased colorectal cancer risk when compared with non-smokers carrying GG genotype in combined analysis. Individuals with family history of cancer harboring LEPR rs12037879 A allele showed 1.52-fold (95%CI: 1.24-fold to 1.86-fold) increased colorectal cancer risk, compared with individuals without family history of cancer harboring GG genotype. Multifactor gene-environment interaction analysis revealed significant interactions among LEPR rs12037879, LEPR rs6690625, smoking status and family history of cancer, exhibiting a gradient of increased colorectal cancer risk along with the increasing number of risk factors (P = 9.82×10−10).Conclusions/Significance
Our research supports that polymorphisms in LEPR may be associated with marginal increase in the risk for colorectal cancer. Moreover, this association could be strengthened by cigarette smoking and family history of cancer. 相似文献86.
长白落叶松生理生态特性的CO2响应及意义 总被引:10,自引:0,他引:10
人工气候室中测定了不同CO2(0-1000mgm^-3)浓度时不同无性系长白落叶松二年生扦插苗的净光合速率,蒸腾速率和水分利用率等,并做了回归分析。结果表明,净光合速率和水分利用率随CO2浓度的增加而升高,蒸腾速度随CO2浓度的增加而降低,但不同无性系的CO2响应方式及强度不同,不同无性系长白落叶松的CO2补偿点亦不同,这些差异是无性系选择的基础上,在无性系选择中,净光合速率和水分利用率的CO2响 相似文献
87.
Nie L Feng W Diaz R Gratton MA Doyle KJ Yamoah EN 《The Journal of biological chemistry》2005,280(15):15097-15102
L-Alpha-difluoromethylornithine (DFMO) is a chemopreventive agent for colon cancer in clinical trials. Yet, the drug produces an across-frequency elevation of the hearing threshold, suggesting that DFMO may affect a common trait along the cochlear spiral. The mechanism for the ototoxic effects of DFMO remains uncertain. The cochlear duct is exclusively endowed with endocochlear potential (EP). EP is a requisite for normal sound transduction, as it provides the electromotive force that determines the magnitude of the receptor potential of hair cells. EP is generated by the high throughput of K(+) across cells of the stria vascularis, conferred partly by the activity of Kir4.1 channels. Here, we show that the ototoxicity of DFMO may be mediated by alteration of the inward rectification of Kir4.1 channels, resulting in a marked reduction in EP. These findings are surprising given that the present model for EP generation asserts that Kir4.1 confers the outflow of K(+) in the stria vascularis. We have proposed an alternative model. These findings should also enable the rational design of new pharmaceuticals devoid of the untoward effect of DFMO. 相似文献
88.
清香桂碱D和矮陀陀胺碱A,B的结构 总被引:9,自引:0,他引:9
本文报道从国产清香桂(Sarcococca ruscifolta)和金丝矮陀陀(Pachysandra axillaria)植物中分得的三个胺碱型新甾体生物碱清香桂碱 D 和矮陀陀胺碱 A、B 的化学结构,并首次归属了它们的~(13)C NMR 数据。 相似文献
89.
Possible target‐related proteins and signal network of bufalin in A549 cells suggested by both iTRAQ‐based and label‐free proteomic analysis 下载免费PDF全文
Dong‐Mei Zhang Li‐Xing Feng Miao Liu Wen‐Hai Jin Ji Luo Ai‐Ying Nie Yue Zhou Yin Li Wan‐Ying Wu Bao‐Hong Jiang Min Yang Xuan Liu 《Proteomics》2016,16(6):935-945
Bufalin (BF) exhibited antiproliferation and antimigration effects on human A549 lung cancer cells. To search its target‐related proteins, protein expression profiles of BF‐treated and control cells were compared using two quantitative proteomic methods, iTRAQ‐based and label‐free proteomic analysis. A total of 5428 proteins were identified in iTRAQ‐based analysis while 6632 proteins were identified in label‐free analysis. The number of common identified proteins of both methods was 4799 proteins. By application of 1.20‐fold for upregulated and 0.83‐fold for downregulated cutoff values, 273 and 802 differentially expressed proteins were found in iTRAQ‐based and label‐free analysis, respectively. The number of common differentially expressed proteins of both methods was 45 proteins. Results of bioinformational analysis using MetacoreTM showed that the two proteomic methods were complementary and both suggested the involvement of oxidative stress and regulation of gene expression in the effects of BF, and fibronectin‐related pathway was suggested to be an important pathway affected by BF. Western blotting assay results confirmed BF‐induced change in levels of fibronectin and other related proteins. Overexpression of fibronectin by plasmid transfection ameliorated antimigration effects of BF. Results of the present study provided information about possible target‐related proteins and signal network of BF. 相似文献
90.
In this paper, an aqueous solution diffusion-localized platform (ASDLP) for multianalyte immunogold staining assays has been developed for the first time by assembling nitrocellulose (NC) strips onto a superhydrophobic polycarbonate (PC) coating with a water contact angle (CA) up to 160°. In the concept-of-proof experiments, the ASDLP was used for colorimetric detection of a human IgG model antigen based on the gold-enhanced gold nanoparticle (AuNP) label amplification. The relative concentration of the analyte captured on NC was further quantified by measuring the intensity of staining result with the use of image analysis software. The comparison study demonstrates that the white superhydrophobic PC-based ASDLP can offer preferable advantages over the commonly adopted bulky piece of NC for immunogold staining assays, in terms of the localized antibody immobilization and reagent addition, the minimization of "coffee effect", uniformity of staining results, quantitative analysis and use efficiency of NC. Moreover, the high selectivity of a multiple antibodies-immobilized NC strip array for multiple antigens in a single sample has been further demonstrated in the multianalyte immunogold staining assay experiments. 相似文献