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排序方式: 共有368条查询结果,搜索用时 140 毫秒
361.
Garth L. Nicolson Adele B. Brodginski Gillian Beattie Ryuzo Yanagimachi 《Molecular reproduction and development》1979,2(2):153-162
Differences in the exposure of spermatozoa surface components during epididymal passage have been examined using lactoperoxidase-catalyzed 125I-iodination or labeling with 125I-diazodiiodosulfanilic acid. Labeled surface proteins obtained from caput and cauda epididymides were solubilized in detergent, separated by sodium dodecylsulfate polyacrylamide slab gel electrophoresis, and identified by radiography. Densitometer scans of autoradiograms revealed increased amounts or exposures of surface proteins of ~35,000, ~39,000, ~50,000, and ~78,000 molecular weight on the cauda epididymal spermatozoa. 相似文献
362.
Calcium requirement for sperm-egg fusion in mammals 总被引:2,自引:0,他引:2
R Yanagimachi 《Biology of reproduction》1978,19(5):949-958
363.
Polyspermy-preventing mechanisms in the golden hamster egg 总被引:2,自引:0,他引:2
364.
R Yanagimachi 《Journal of reproduction and fertility》1966,11(3):359-370
365.
To determine the changes in patterns of 17 beta-estradiol and progesterone levels underlying abnormal cycles in bitches immunized with solubilized crude porcine zonae pellucidae (cPZP), to attempt to circumvent these problems by immunizing with a purified zona fraction (pPZP), and to test the effectiveness of different adjuvants, bitches were immunized with cPZP or pPZP 2-6 times with no adjuvant, Freund's adjuvant, alum adjuvant, or the adjuvant CP-20,961. The bitch immunized without adjuvant had a low titer with a normal cycle and fertility. Immunization with cPZP and adjuvant produced moderate to high titers of antizona antibodies and infertility. Bitches with high titers experienced abnormal estrous cycles. Estradiol rose during proestrus, but instead of falling sharply in early estrus as in controls, it remained elevated. Progesterone did not rise. The moderate-titered bitches had normal cycles and steroid patterns. Bitches immunized with pPZP had moderate titers. Cycles were normal after 3 injections, but after 6 injections one bitch had an abnormal cycle. One pPZP-immunized bitch remained fertile but the others were infertile. Alum was the mildest adjuvant, causing no injection site lesions, but the highest titers occurred with Freund's and CP-20,961 adjuvants. All three adjuvants induced titers sufficient to inhibit fertility. Infertility in bitches immunized with PZP may be due to prevention of zona penetration, because their antisera inhibited zona penetration of oocytes by spermatozoa in vitro. However, alterations in ovarian function preventing ovulation and luteinization could be involved in high-titered bitches. 相似文献
366.
Isolation and characterization of indene bioconversion genes from Rhodococcus strain I24 总被引:3,自引:0,他引:3
S. L. Treadway K. S. Yanagimachi E. Lankenau P. A. Lessard G. Stephanopoulos A. J. Sinskey 《Applied microbiology and biotechnology》1999,51(6):786-793
Rhodococcus strain I24 is able to convert indene into indandiol via the actions of at least two dioxygenase systems and a putative monooxygenase
system. We have identified a cosmid clone from I24 genomic DNA that is able to confer the ability to convert indene to indandiol
upon Rhodococcus erythropolis SQ1, a strain that normally can not convert or metabolize indene. HPLC analysis reveals that the transformed SQ1 strain produces
cis-(1R,2S)-indandiol, suggesting that the cosmid clone encodes a naphthalene-type dioxygenase. DNA sequence analysis of a portion of
this clone confirmed the presence of genes for the dioxygenase as well as genes encoding a dehydrogenase and putative aldolase.
These genes will be useful for manipulating indene bioconversion in Rhodococcus strain I24.
Received: 8 December 1998 / Received revision: 26 January 1999 / Accepted: 5 February 1999 相似文献
367.
368.