全文获取类型
收费全文 | 144篇 |
免费 | 13篇 |
国内免费 | 21篇 |
出版年
2023年 | 3篇 |
2022年 | 13篇 |
2021年 | 9篇 |
2020年 | 7篇 |
2019年 | 12篇 |
2018年 | 5篇 |
2017年 | 7篇 |
2016年 | 6篇 |
2015年 | 15篇 |
2014年 | 12篇 |
2013年 | 11篇 |
2012年 | 12篇 |
2011年 | 5篇 |
2010年 | 5篇 |
2009年 | 4篇 |
2008年 | 2篇 |
2007年 | 7篇 |
2006年 | 7篇 |
2005年 | 12篇 |
2004年 | 2篇 |
2003年 | 3篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1980年 | 1篇 |
排序方式: 共有178条查询结果,搜索用时 31 毫秒
151.
Xinyue Liu Yamin Xu Kang Cheng Juling Long Weichao Huang Chaoyong Deng 《Luminescence》2023,38(6):692-701
A novel far-red emitting phosphor Sr2MgWO6: Mn4+ was fabricated using high-temperature solid-state reaction. X-ray diffraction patterns, scanning electron microscopy images, and photoluminescence excitation and photoluminescence spectra for this phosphor were analyzed in detail. The analysis revealed that its emission ranged from 600 to 800 nm and peaked at 699 nm, which was attributed to the 2Eg→4A2g transition of Mn4+ under 314 nm excitation. Moreover, we introduced rare-earth Yb3+ ions into the Sr2MgWO6:Mn4+ to improve its far-red emitting intensity. The photoluminescence (PL) intensity of the Yb3+ co-doped phosphor was three times higher than that of the single-doped phosphor. Therefore charge compensation is an efficient approach to improving PL intensity. The phosphor emitted a far-red light that resembled the pigments essential for plant growth in terms of the absorption spectrum. Therefore, the obtained phosphor, Sr2MgWO6:0.006Mn4+,0.2Yb3+, had the potential to be a new type of far-red luminescent powder for indoor plant growth LEDs. 相似文献
152.
向日葵黑斑病(Alternaria helianthi(Hansford)Tubakiyet Nishihasa)在世界上分布较广,危害也较大,我国东北三省都有发生,近年在吉林省白城市向日葵主产区发生严重,因感此病而发生大范围枯死,严重影响向日葵籽实产量和质量。由于向日葵植株高大,化学防 相似文献
153.
Yamin Zhang Huanran Feng Lingxian Meng Yanbo Wang Meijia Chang Shitong Li Ziqi Guo Chenxi Li Nan Zheng Zengqi Xie Xiangjian Wan Yongsheng Chen 《Liver Transplantation》2019,9(45)
Developing efficient organic solar cells (OSCs) with relatively thick active layer compatible with the roll to roll large area printing process is an inevitable requirement for the commercialization of this field. However, typical laboratory OSCs generally exhibit active layers with optimized thickness around 100 nm and very low thickness tolerance, which cannot be suitable for roll to roll process. In this work, high performance of thick‐film organic solar cells employing a nonfullerene acceptor F–2Cl and a polymer donor PM6 is demonstrated. High power conversion efficiencies (PCEs) of 13.80% in the inverted structure device and 12.83% in the conventional structure device are achieved under optimized conditions. PCE of 9.03% is obtained for the inverted device with active layer thickness of 500 nm. It is worth noting that the conventional structure device still maintains the PCE of over 10% when the film thickness of the active layer is 600 nm, which is the highest value for the NF‐OSCs with such a large active layer thickness. It is found that the performance difference between the thick active layer films based conventional and inverted devices is attributed to their different vertical phase separation in the active layers. 相似文献
154.
Longquan Zuo Yueqin Zhu Lili Hu Yanyi Liu Yinghong Wang Yamin Hu Huan Wang Xuesheng Pan Kuayue Li Na Du Yan Huang 《Journal of cellular and molecular medicine》2019,23(6):3940-3950
Acid‐sensing ion channel 1a (ASIC1a) allows Na+ and Ca2+ flow into cells. It is expressed during inflammation, in tumour and ischaemic tissue, in the central nervous system and non‐neuronal injury environments. Endoplasmic reticulum stress (ERS) is caused by the accumulation of misfolded proteins that interferes with intracellular calcium homoeostasis. Our recent reports showed ASIC1a and ERS are involved in liver fibrosis progression, particularly in hepatic stellate cell (HSC) activation. In this study, we investigated the roles of ASIC1a and ERS in activated HSC. We found that ASIC1a and ERS‐related proteins were up‐regulated in carbon tetrachloride (CCl4)‐induced fibrotic mouse liver tissues, and in patient liver tissues with hepatocellular carcinoma with severe liver fibrosis. The results show silencing ASIC1a reduced the expression of ERS‐related biomarkers GRP78, Caspase12 and IREI‐XBP1. And, ERS inhibition by 4‐PBA down‐regulated the high expression of ASIC1a induced by PDGF, suggesting an interactive relationship. In PDGF‐induced HSCs, ASIC1a was activated and migrated to the cell membrane, leading to extracellular calcium influx and ERS, which was mediated by PI3K/AKT pathway. Our work shows PDGF‐activated ASIC1a via the PI3K/AKT pathway, induced ERS and promoted liver fibrosis progression. 相似文献
155.
Ziv Alperovich Gal Yamin Eliav Elul Gabriel Bialolenker Amiel A. Ishaaya 《Journal of biophotonics》2019,12(9)
We suggest a novel method to classify the type of tissue that is being ablated, using the recorded acoustic sound waves during pulsed ultraviolet laser ablation. The motivation of the current research is tissue classification during vascular interventions, where the identification of the ablated tissue is vital. We classify the acoustic signatures using Mel‐frequency cepstral coefficients (MFCCs) feature extraction with a Support Vector Machine (SVM) algorithm, and in addition, use a fully connected deep neural network (FC‐DNN) algorithm. First, we classify three different liquids using our method as a preliminary proof of concept. Then, we classify ex vivo porcine aorta and bovine tendon tissues in the presence of saline. Finally, we classify ex vivo porcine aorta and bovine tendon tissues where the acoustic signals are recorded through chicken breast medium. The results for tissue classification in saline and through chicken breast both show high accuracy (>98%), based on tens of thousands of acoustic signals for each experiment. The experiments were conducted in a noisy and challenging setting that tries to imitate practical working conditions. The obtained results could pave the way towards practical tissue classification in various important medical procedures, achieving enhanced efficacy and safety. 相似文献
156.
Qiling Li Yamin Ma Wenzhi Li Wei Xu Li Ma Guoxing Fu Xiaohua Tian Yueling Wang Xu Li Tameka Bythwood Jendai Richards Mukaila A. Akinbami Qing Song 《Transgenic research》2014,23(2):341-349
Gender-preferential gene expression is a widespread phenomenon in humans. It is important to study how gender differences influence the pathogenesis of various diseases and response to specific drugs. The aim of this study is to determine if the mouse albumin enhancer/promoter may serve as the promoter to introduce gender-preferential gene expression in transgenic animals. We created four independent transgenic rat lines in which the human C-reactive protein transgene was under the control of mouse albumin enhancer/promoter. Quantitative real time RT-PCR analysis showed that transgene expression in the liver of male rats was significantly higher than transgene expression in the female rats (P < 0.05).There was a 5.3-fold (male/female) difference in line-519, and a 12.2-fold (male/female) difference in line-488. Enzyme-linked immunosorbent assay showed that the serum of male transgenic rats had a 13- to 679-fold difference at the protein level on transgene production compared with female transgenic rats. The male-to-female difference in gene expression was 10- to 17-fold in the liver of transgenic rats. Orchiectomy dramatically reduced protein production from the transgene in the liver. Testosterone administration into female rats did not increase the transgene expression, but estrogen administration into the male rats reduced transgene expression. This study provides a valuable tool for investigating the pathological roles of genes that are expressed in a gender-preferential manner in human disease. 相似文献
157.
从天山雪莲叶片低温诱导的cDNA文库中克隆了一个未知蛋白基因(SikSD-82)cDNA全长序列。生物信息学分析表明SikSD-82基因共编码262个氨基酸,具有小麦铝诱导蛋白Wali7的保守结构域,属Gn_AT_Ⅱ家族的可溶性蛋白;其氨基酸序列与蓖麻的茎部特异性表达蛋白相似性最高为60.89%;进化树分析显示,该蛋白与番茄的茎部特异性表达蛋白关系最近。亚细胞定位结果表明,SikSD-82蛋白主要定位于细胞核。在铝胁迫条件下,转SikSD-82烟草幼苗根的伸长明显优于野生型,根部铝离子和丙二醛含量均显著低于野生型烟草,转SikSD-82烟草显示出较强的耐铝性。 相似文献
158.
Background
Few reports have examined the viral aetiology of fever of unknown origin (FUO).Objective
This study determined the prevalence of human herpes virus (HHV) DNA in blood of Chinese patients with classic FUO using the polymerase chain reaction (PCR) and explored the possible role of HHV.Study design
Blood samples were collected from 186 patients (151 children, 35 adults) with classic FUO and 143 normal individuals in Beijing during the years 2009–2012. The HHV DNA, including Herpes simplex virus (HSV)-1/2, Varicella zoster virus (VZV), Cytomegalovirus (CMV), Epstein–Barr virus (EBV), and Human herpes virus (HHV)-6 and -7, was detected by multiplex PCR. The epidemiological and clinical features were also analysed.Results
HHV DNA was detected in 63 (33.9%) of the FUO patients, and the prevalence of EBV and HHV-6 was significantly higher than in the normal cohort. HHV co-infection was also frequent (10.2%) in the patients with FUO. The majority of patients with HHV infection present with a fever only. Our data also revealed that EBV infection was associated with hepatitis and abnormal blood indices, HHV-6 was associated with a cough, and HHV-7 was associated with hepatitis.Conclusions
HHVs are associated with Chinese patients (especially for children) with classic FUO. Our study adds perspective to the aetiological and clinical characteristics of classic FUO in beijing patients. 相似文献159.
以3株国内分离的亚洲1型口蹄疫病毒(分别命名为F1、F2、F3)为研究目标,根据GenBank中注册的FMDV VP1基因的序列设计1对引物,采用RT-PCR方法成功地扩增出含有VP1全基因的片段,并测定了3个毒株VP1基因的序列.结果表明,3株亚洲1型FMDV毒株VP1基因长度均为633 bp,编码211个氨基酸.3株毒株彼此之间的核苷酸序列同源性在82.8% ~99.1%之间,推导氨基酸序列同源性在89.1% ~99.1%之间.从系统发生树看,F1株与我国香港2005年牛毒株序列同源性99.5%,属同一遗传谱系,F2株、F3株与2005年引起河北省万全县、北京市延庆县、甘肃静宁县疫情的毒株分属同一个基因群. 相似文献
160.
桑粒肩天牛幼虫肠道菌群的研究 总被引:22,自引:0,他引:22
Intestinal flora of 47 Apriona germari(Hope) larvae,collected from fields,had been isolated and identified.The results showed that the predominant bacteria were Staphylococcus.Its viable count was 7.63±0.21,and the detection rate was 100%.Meanwhile,a strain of cellulose-utilizing bacterium was isolated from the fore-midgut fluid of A.germari larvae with the cellulose-congo red agar medium.The bacterium was tentatively identified as Cellulomonas.The detection rate of the cellulolytic bacterium was 23.40%,and… 相似文献