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71.
Youhei Murakami Shin Kamiyama Zakir H Howlader Tokuhisa Yamato Michio Komai Yuji Furukawa 《Journal of biochemistry》2002,131(1):17-19
We tried to confirm the antioxidative capability of lecithin:cholesterol acyltransferase (LCAT) reported by Vohl et al. [Biochemistry (1999) 38, 5976-5981]. The enzyme solution protected LDL against oxidation. However, this protection was not due to LCAT enzyme, but to some unknown low-molecular-weight substance(s) in the solution; LCAT itself exerted little protective effect against LDL oxidation. 相似文献
72.
Fusao Ota Masayuki Yamato Mie Hayashi Masayuki Ota Tetsuro Koga Ahmed Sherin Chiharu Mukai Kentaro Sakai Shigeru Yamamoto 《Microbiological research》2002,157(4):305
Twenty four reference strains (serotype a-h) belonging to the mutans group of streptococci were compared for DNA fragment patterns of rDNA after treatment with Hind III. It was shown that Streptococcus cricetus (serotype a), S. rattus (serotype b), and S. downei (serotype h) reveals comparatively homogeneous patterns while S. mutans (serotype c, e and f) exhibits differences between the different serotypes as well as within single serotypes. S. sobrinus had an intermediary diversity. These data support the previous findings that S. mutans is heterogeneous at the serological, biochemical and genetical level. 相似文献
73.
Emiko Nishi Nami Suzuki-Hashido Takashi Hayakawa Yamato Tsuji Bambang Suryobroto Hiroo Imai 《Primates; journal of primatology》2018,59(6):523-530
For many primates, sweet taste is palatable and is an indicator that the food contains carbohydrates, such as sugars and starches, as energy sources. However, we have found that Asian colobine monkeys (lutungs and langurs) have low sensitivity to various natural sugars. Sweet tastes are recognized when compounds bind to the sweet taste receptor TAS1R2/TAS1R3 in the oral cavity; accordingly, we conducted a functional assay using a heterologous expression system to evaluate the responses of Javan lutung (Trachypithecus auratus) TAS1R2/TAS1R3 to various natural sugars. We found that Javan lutung TAS1R2/TAS1R3 did not respond to natural sugars such as sucrose and maltose. We also conducted a behavioral experiment using the silvery lutung (Trachypithecus cristatus) and Hanuman langur (Semnopithecus entellus) by measuring the consumption of sugar-flavored jellies. Consistent with the functional assay results for TAS1R2/TAS1R3, these Asian colobine monkeys showed no preference for sucrose or maltose jellies. These results demonstrate that sweet taste sensitivity to natural sugars is low in Asian colobine monkeys, and this may be related to the specific feeding habits of colobine monkeys. 相似文献
74.
Taisuke Togawa Tohru Adachi Daijiro Harada Tasuku Mitani Daisuke Tanaka Kimitsune Ishizaki Takayuki Kohchi Katsuyuki T. Yamato 《Journal of plant research》2018,131(6):1047-1054
The liverwort Marchantia polymorpha has become one of the model organisms, since it has less genetic redundancy, sexual and asexual modes of reproduction and a range of genomic and molecular genetic resources. Cryopreservation of fertile spermatozoa eliminates time, space and labor for growing and maintaining male plants in reproductive phase, and also provides an optional way to backup lines. Here we report a protocol to cryopreserve spermatozoa of M. polymorpha in liquid nitrogen. A cryoprotective solution containing sucrose, glycerol and egg yolk and controlled cooling and warming processes led to successful recovery of motile M. polymorpha spermatozoa after the cryogenic process. The survival rate and average motility of spermatozoa after cryopreservation were maintained at 71 and 54% of those before cryopreservation, respectively. Cryopreserved spermatozoa were capable of fertilization to form normal spores. The technique presented here confers more versatility to experiments using M. polymorpha and could be applied to preservation of plant spermatozoa in general. 相似文献
75.
SAD‐B kinase regulates pre‐synaptic vesicular dynamics at hippocampal Schaffer collateral synapses and affects contextual fear memory 下载免费PDF全文
76.
Temporal changes in food availability affect foraging success and ultimately reproductive success of animals. They include
both seasonal and annual changes. Although many researchers have investigated food availability and the corresponding ranging
behavior of primates, studies of yearly changes have been limited. We studied the effects of the fruiting of nuts of Fagus crenata, Zelkova serrata, and Torreya nucifera on the ranging behavior of Japanese macaques (Macaca fuscata) in autumn on Kinkazan Island, northern Japan, for 5 yr between 2000 and 2005 (excluding 2003). We divided the study area
(3.3 km2) into 100 × 100-m quadrats to assess the distribution of nut-rich quadrats that contained high densities of nut-producing
species. The home ranges of the monkeys contained more nut-rich quadrats than expected, and the difference was significant
for 3 of 5 yr. Autumn home-range use was also affected by the distribution of nut production, although the effect differed
by year: monkeys frequently used nut-rich quadrats in a Zelkova-year (2000) and in 1 Torreya-year (2001), whereas they nonselectively used nut-rich quadrats in 2 Fagus-years (2002 and 2005) and in another Torreya-year (2004). Thus, Japanese macaques flexibly change their autumn ranging behavior according to yearly changes in distribution
of nut production. 相似文献
77.
78.
Shinozuka T Shimada K Matsui S Yamane T Ama M Fukuda T Taki M Takeda Y Otsuka E Yamato M Mochizuki S Ohhata K Naito S 《Bioorganic & medicinal chemistry》2006,14(20):6789-6806
A novel series of cathepsin K inhibitors derived from Novartis compound I is described. Optimization of the P1, P3, and P1' units led to the identification of 4-aminophenoxyacetic acid 24b with an IC(50) value of 4.8 nM, which possessed an excellent selectivity over other human cathepsins and good pharmacokinetic (PK) properties. Oral administration of compound 24b to ovariectomized (OVX) rats showed a trend toward an improvement of bone mineral density (BMD) in the femur bone. 相似文献
79.
Masahiro Yamasaki Yusuke Kobayashi Kensuke Nakamura Noboru Sasaki Masahiro Murakami Bandula Kumara Wickramasekara Rajapakshage Hiroshi Ohta Osamu Yamato Yoshimitsu Maede Mitsuyoshi Takiguchi 《Experimental parasitology》2011,127(1):119-126
In this study, we attempted to detect Babesia gibsoni in blood smears and formalin-fixed, paraffin-embedded tissues obtained from B. gibsoni-infected dogs using in situ hybridization. Using a digoxigenin-conjugated deoxyribonucleic acid (DNA) probe, both intraerythrocytic and exoerythrocytic parasites in the culture could be specifically stained in blood smears fixed with 4% phosphate-buffered paraformaldehyde. This indicated that genomic DNA extracted from the parasites could be detected using in situ hybridization. Moreover, the parasite could be specifically stained in paraffin-embedded spleen, lymph node, and kidney sections using in situ hybridization. Infected erythrocytes in blood vessels in the spleen and kidney, hemosiderin-laden macrophages in the spleen, and phagocytized erythrocytes, which seemed to be infected with the parasites, in lymph nodes were also specifically stained. This suggests that in situ hybridization can be utilized to investigate both the life cycle of B. gibsoni and the pathological condition of canine babesiosis. 相似文献
80.