全文获取类型
收费全文 | 161篇 |
免费 | 21篇 |
国内免费 | 1篇 |
出版年
2022年 | 1篇 |
2020年 | 1篇 |
2018年 | 1篇 |
2017年 | 1篇 |
2016年 | 3篇 |
2015年 | 1篇 |
2014年 | 6篇 |
2013年 | 11篇 |
2012年 | 11篇 |
2011年 | 14篇 |
2010年 | 8篇 |
2009年 | 7篇 |
2008年 | 2篇 |
2007年 | 9篇 |
2006年 | 10篇 |
2005年 | 4篇 |
2004年 | 12篇 |
2003年 | 8篇 |
2002年 | 6篇 |
2001年 | 4篇 |
2000年 | 7篇 |
1999年 | 8篇 |
1998年 | 3篇 |
1997年 | 5篇 |
1996年 | 5篇 |
1995年 | 4篇 |
1994年 | 10篇 |
1993年 | 9篇 |
1992年 | 7篇 |
1990年 | 2篇 |
1987年 | 1篇 |
1986年 | 2篇 |
排序方式: 共有183条查询结果,搜索用时 19 毫秒
31.
Mikami K; Takahashi S; Katagiri T; Yamaguchi-Shinozaki K; Shinozaki K 《Journal of experimental botany》1999,50(334):729-730
Screening of an Arabidopsis cDNA library allowed the
isolation of a cDNA encoding a pleckstrin homology (PH) domain protein,
AtPH1, which consists of one PH domain with a short N-terminal extension.
According to its structural features, AtPH1 is proposed to be a plant
homologue of human pleckstrin. Northern blot analysis indicated that the
AtPH1 gene was expressed constitutively in all tissues
examined, with variation in the levels. The presence of a plant pleckstrin
homologue offers new insights into the biological function of the PH domain
in plant signalling. 相似文献
32.
R Motohashi T Ito M Seki K Ichimura K Yamaguchi-Shinozaki K Shinozaki 《DNA research》1999,6(4):247-253
We previously reported a cDNA selection method using DNA latex particles to identify expressed genes in specific regions of genomes and named this cDNA scanning method (Hayashida et al., 1995 Gene 155 161). We applied the cDNA scanning method to the YAC CIC3B1-S DNA on Arabidopsis thaliana chromosome 5, and constructed a region-specific sublibrary in which cDNAs for genes on the YAC CIC3B1-S DNA were concentrated. We isolated 545 cDNA clones from the sublibrary, and determined partial sequence of them to produce expressed sequence tags (ESTs) derived from the YAC region. In total, 74 nonredundant groups of cDNAs were obtained from 545 cDNA clones. Forty-seven percent of these EST clones had significant homology to functional proteins such as protein kinases, LON protease, nucleic acid binding protein and chloride channel protein. We compared the cDNA sequences isolated by the cDNA scanning method to the Arabidopsis genomic sequence corresponding to the YAC CIC3B1-S region, and found that 69% of the selected cDNAs are located in the region. We discuss the fidelity and efficiency of the cDNA scanning method for cloning region-specific cDNAs and its useful application in positional cloning. 相似文献
33.
Kazuo Nakashima Tomohiro Kiyosue Kazuko Yamaguchi-Shinozaki Kazuo Shinozaki 《The Plant journal : for cell and molecular biology》1997,12(4):851-861
A cDNA, ERD1, isolated from one-hour-dehydrated plants of Arabidopsis thaliana L. encodes a putative protein that is similar to the regulatory ATPase subunit (ClpA) of the Clp protease and contains a putative chloroplast-targeting transit-peptide at the N-terminus. A chimeric gene with the putative plastid-targeting sequence of the erd1 gene fused to the synthetic green-fluorescent protein (sGFP) gene was constructed and introduced into Arabidopsis protoplasts. The N-terminal region of the ERD1 protein directed the sGFP protein into the plastids of the protoplasts, and functioned as a transit peptide. Northern blot analysis indicated that expression of the erd1 gene was induced not only by water stress, such as dehydration and high salinity, but also by natural senescence and dark-induced etiolation. The erd1 gene was not strongly induced by exogenous abscisic acid. A chimeric gene with the 0.9 kb promoter region of the erd1 gene fused to the β-glucuronidase (GUS) reporter gene was constructed, and tobacco plants transformed with the construct. The GUS reporter gene driven by the erd1 promoter was induced by dehydration and high salt stress at significant levels in the transgenic plants. The GUS gene was strongly expressed in older leaves without dehydration, and was induced by dark-induced etiolation. Furthermore, GUS activity was reduced by cytokinin treatment during dark-induced etiolation. These results indicate that expression of the erd1 gene is developmentally up-regulated by senescence as well as by water stress. 相似文献
34.
35.
36.
37.
38.
39.
40.