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161.
Takuma Ishizaki Kyonoshin Maruyama Mitsuhiro Obara Akiyo Fukutani Kazuko Yamaguchi-Shinozaki Yusuke Ito Takashi Kumashiro 《Molecular breeding : new strategies in plant improvement》2013,31(2):255-264
Dehydration-responsive-element-binding protein 1 genes have important roles in response to stress. To improve the drought tolerance of an upland rice cultivar NERICA1, we introduced Arabidopsis AtDREB1C or rice OsDREB1B driven by a stress-inducible rice lip9 promoter. Plants of some transgenic lines survived better than non-transgenic plants under severe drought. AtDREB1C transgenic plants had higher dry weights than non-transgenic plants when grown under moderate drought until the late vegetative growth stage. On the other hand, OsDREB1B transgenic plants had lower dry weights than non-transgenic plants under the same condition. Similar results were obtained under osmotic stress. The AtDREB1C transgenic plants headed earlier, had a larger sink capacity, and had more filled grains than non-transgenic plants. These results suggest that AtDREB1C expressed in NERICA1 improves not only survival under severe drought, but also growth and yield under moderate drought. 相似文献
162.
Two Distinct Families of Protein Kinases Are Required for Plant Growth under High External Mg2+ Concentrations in Arabidopsis 总被引:1,自引:0,他引:1
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Tsuyoshi Mizoguchi Yukiko Gotoh Eisuke Nishida Kazuko Yamaguchi-Shinozaki Nobuaki Hayashida Toshisuke Iwasaki Hiroshi Kamada Kazuo Shinozaki 《The Plant journal : for cell and molecular biology》1994,5(1):111-122
Two cDNA clones, cATMPK1 and CATMPK2, encoding MAP kinases (mitogen-activated protein kinases) have been cloned from Arabidopsis thaliana and their nucleotide sequences have been determined. Putative proteins encoded by ATMPK1 and ATMPK2 genes, designated ATMPK1 and ATMPK2, contain 370 and 376 amino acid residues, respectively, and are 88.7% identical at the amino acid sequence level. ATMPK1 and ATMPK2 exhibit significant similarity to rat ERK2 (49%) and Xenopus MAP kinase (50%). The amino acid residues corresponding to the sites of phosphorylation (Thr-Glu-Tyr) that are involved in the activation of MAP kinases are conserved in ATMPK1 and ATMPK2. Northern blot analysis indicates that the ATMPK1 and ATMPK2 mRNAs are significantly present in all the organs except seeds. Genomic Southern blot analysis suggests that there are a few additional genes that are related to ATMPK1 and ATMPK2 in the Arabidopsis genome. Purified Xenopus MAP kinase kinase (MAPK kinase) phosphorylates ATMPK1 and ATMPK2 proteins that have been expressed in Escherichia coli, activating these enzymes. A rapid and transient activation of 46-kDa protein kinase activity that phosphorylated myelin basic protein (MBP) was detected when auxinstarved tobacco BY-2 cells were treated with synthetic auxin, 2,4-dichlorophenoxyacetic acid (2,4-D). Protein kinase activities which phosphorylated the recombinant ATMPK2 protein also increased rapidly after auxin treatment in the auxin-starved BY-2 cells. These results suggest that auxin may function as an activator of plant MAP kinase homologues, as do various mitogens in animal systems. 相似文献
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ABA signaling in stress-response and seed development 总被引:5,自引:0,他引:5
168.
Characterization of an Arabidopsis cDNA for a soluble epoxide hydrolase gene that is inducible by auxin and water stress 总被引:3,自引:1,他引:3
169.
Molecular responses to drought,salinity and frost: common and different paths for plant protection 总被引:43,自引:0,他引:43
Seki M Kamei A Yamaguchi-Shinozaki K Shinozaki K 《Current opinion in biotechnology》2003,14(2):194-199
Drought, high salinity and low temperature are major environmental factors that limit plant productivity. Plants respond and adapt to these stresses in order to survive. Signaling pathways are induced in response to environmental stress and recent molecular and genetic studies have revealed that these pathways involve many components. In this review, we highlight recent findings on the gene expression associated with stress responses and the signaling pathways that are either common or specific to the response. 相似文献
170.
Arabidopsis HsfA1 transcription factors function as the main positive regulators in heat shock-responsive gene expression 总被引:1,自引:0,他引:1