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Mustafa Kemal Altunoglu Adem Bicakci Sevcan Celenk Yakup Canitez Hulusi Malyer Nihat Sapan 《Biologia》2008,63(5):658-663
In this study, airborne pollen grains of Yalova province were investigated using VPSS 2000 from January to December 2004.
During studying period, a total of 22409 pollen grains/m3 which belonged to 46 taxa and 74 unidentified pollen grains were recorded. From the identified taxa, 26 belong to arboreal
and 20 to non-arboreal plants. Total pollen grains consist of 80.50% arboreal, 19.17% non-arboreal plants and 0.33% unidentified
pollen grains. In the investigated region, from arboreal plant taxa Platanus spp. (29.08%), Cupressaceae/Taxaceae (21.22%), Pinus spp. (7.34%), Alnus spp. (4.75%), Castanea spp. (3.03%), Quercus spp. (3.07%), Olea spp. (2.50%), Acer spp. (2.21%), Corylus spp. (1.41%) and Fagus spp. (1.15%), and from non-arboreal plant taxa Poaceae (10.01%), Asteraceae (2.86%), Plantago spp. (1.47%) and Artemisia spp. (1.11%) were responsible for the greatest amounts of pollen. 相似文献
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Bayramoglu G Karagoz B Altintas B Arica MY Bicak N 《Bioprocess and biosystems engineering》2011,34(6):735-746
Fibrous poly(styrene-b-glycidylmethacrylate) brushes were grafted on poly(styrene–divinylbenzene) (P(S–DVB)) beads using surface-initiated atom
transfer radical polymerization. Tetraethyldiethylenetriamine (TEDETA) ligand was incorporated on P(GMA) block. The ligand
attached beads were used for reversible immobilization of lipase. The influences of pH, ionic strength, and initial lipase
concentration on the immobilization capacities of the beads have been investigated. Lipase adsorption capacity of the beads
was about 78.1 mg/g beads at pH 6.0. The K
m value for immobilized lipase was about 2.1-fold higher than that of free enzyme. The thermal, and storage stability of the
immobilized lipase also was increased compared to the native lipase. It was observed that the same support enzyme could be
repeatedly used for immobilization of lipase after regeneration without significant loss in adsorption capacity or enzyme
activity. A lipase from Mucor miehei immobilized on styrene–divinylbenzene copolymer was used to catalyze the direct esterification of butyl alcohol and butyric
acid. 相似文献
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Tavaré R Sagoo P Varama G Tanriver Y Warely A Diebold SS Southworth R Schaeffter T Lechler RI Razavi R Lombardi G Mullen GE 《PloS one》2011,6(5):e19662
Dendritic cells (DCs) generated in vitro to present tumour antigens have been injected in cancer patients to boost in vivo anti-tumour immune responses. This approach to cancer immunotherapy has had limited success. For anti-tumour therapy, delivery and subsequent migration of DCs to lymph nodes leading to effective stimulation of effector T cells is thought to be essential. The ability to non-invasively monitor the fate of adoptively transferred DCs in vivo using magnetic resonance imaging (MRI) is an important clinical tool to correlate their in vivo behavior with response to treatment. Previous reports of superparamagnetic iron oxides (SPIOs) labelling of different cell types, including DCs, have indicated varying detrimental effects on cell viability, migration, differentiation and immune function. Here we describe an optimised labelling procedure using a short incubation time and low concentration of clinically used SPIO Endorem to successfully track murine DC migration in vivo using MRI in a mouse tumour model. First, intracellular labelling of bone marrow derived DCs was monitored in vitro using electron microscopy and MRI relaxometry. Second, the in vitro characterisation of SPIO labelled DCs demonstrated that viability, phenotype and functions were comparable to unlabelled DCs. Third, ex vivo SPIO labelled DCs, when injected subcutaneously, allowed for the longitudinal monitoring by MR imaging of their migration in vivo. Fourth, the SPIO DCs induced the proliferation of adoptively transferred CD4(+) T cells but, most importantly, they primed cytotoxic CD8(+) T cell responses to protect against a B16-Ova tumour challenge. Finally, using anatomical information from the MR images, the immigration of DCs was confirmed by the increase in lymph node size post-DC injection. These results demonstrate that the SPIO labelling protocol developed in this study is not detrimental for DC function in vitro and in vivo has potential clinical application in monitoring therapeutic DCs in patients with cancer. 相似文献
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Koray Ergunay Filiz Gunay Ozge Erisoz Kasap Kerem Oter Sepandar Gargari Taner Karaoglu Seda Tezcan Mehmet Cabalar Yakup Yildirim Gürol Emekdas Bulent Alten Aykut Ozkul 《PLoS neglected tropical diseases》2014,8(7)
West Nile virus (WNV), a mosquito-borne flavivirus with significant impact on human and animal health, has recently demonstrated an expanded zone of activity globally. The aim of this study is to investigate the frequency and distribution of WNV infections in potential vectors and several mammal and avian species in Turkey, where previous data indicate viral circulation. The study was conducted in 15 provinces across Turkey during 2011–2013. In addition, the entomological study was extended to 4 districts of the Turkish Republic of Northern Cyprus. WNV exposure was determined in humans, horses, sheep and ducks from Mersin, Sanliurfa, Van and Kars provinces of Turkey, via the detection of neutralizing antibodies. WNV RNA was sought in human and equine samples from Mersin, Adana and Mugla provinces. Field-collected mosquitoes from 92 sites at 46 locations were characterized morphologically and evaluated for viral RNA. Neutralizing antibodies were identified in 10.5% of the 1180 samples studied and detected in all species evaluated. Viral nucleic acids were observed in 5.9% of 522 samples but only in horses. A total of 2642 mosquito specimens belonging to 15 species were captured, where Ochlerotatus caspius (52.4%), Culex pipiens sensu lato (24.2%) comprise the most frequent species. WNV RNA was detected in 4 mosquito pools (1.9%), that comprise Oc. caspius Cx. pipiens s.l. and DNA barcoding revealed the presence of Cx. quinquefasciatus and Cx. perexiguus mosquitoes in infected Culex pools. All WNV partial sequences were characterized as lineage 1 clade 1a. These findings indicate a widespread WNV activity in Turkey, in Eastern Thrace and Mediterranean-Aegean regions as well as Southeastern and Northeastern Anatolia. 相似文献
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Seda Savranoglu Kulabas Ferah Comert Onder Yakup Berkay Yılmaz Adem Ozleyen Kader Sahin 《Journal of biomolecular structure & dynamics》2020,38(15):4655-4668
Communicated by Ramaswamy H. Sarma 相似文献
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Alicigüzel Y Aktaş S Bozan H Aslan M 《Journal of enzyme inhibition and medicinal chemistry》2005,20(3):293-296
Intravenous nitroglycerin (GTN) has been used as an anti-ischemic agent for the therapy of unstable and post-infarction angina. Nitric oxide (NO) and S-nitrosothiols constitute the biologically active species formed via nitroglycerin bioactivation. Increased levels of reactive oxygen species can diminish the therapeutic action of organic nitrates by scavenging donated NO and oxidizing tissue thiols important in nitrate biotransformation. Studies reported here show that the red cell activity of antioxidant enzymes, catalase and glutathione peroxidase, are significantly decreased after intravenous nitroglycerin treatment. Catalase activity (739.6 +/- 92.3 k/gHb) decreased to 440.1 +/- 111.9 and 459.8 +/- 130.7 k/gHb after 1 and 24 hr GTN infusion, respectively. Similarly, glutathione peroxidase activity (5.8 +/- 1.8 U/gHb) decreased to 3.2 +/- 1.7 and 3.8 +/- 1.1 U/g Hb after 1 and 24 hr GTN infusion, respectively. The reported decrease in antioxidant enzyme activities can lead to an oxidant milieu and contribute to the generation of nitrate tolerance. 相似文献
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