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41.
Ana Paula Junqueira-Kipnis Fábio Muniz de Oliveira Monalisa Martins Trentini Sangeeta Tiwari Bing Chen Danilo Pires Resende Bruna D. S. Silva Mei Chen Lydia Tesfa William R. Jacobs Jr André Kipnis 《PloS one》2013,8(11)
The development of a new vaccine as a substitute for Bacillus Calmette–Guerin or to improve its efficacy is one of the many World Health Organization goals to control tuberculosis. Mycobacterial vectors have been used successfully in the development of vaccines against tuberculosis. To enhance the potential utility of Mycobacterium smegmatis as a vaccine, it was transformed with a recombinant plasmid containing the partial sequences of the genes Ag85c, MPT51, and HspX (CMX) from M. tuberculosis. The newly generated recombinant strain mc2-CMX was tested in a murine model of infection. The recombinant vaccine induced specific IgG1 or IgG2a responses to CMX. CD4+ and CD8+ T cells from the lungs and spleen responded ex vivo to CMX, producing IFN-γ, IL17, TNF-α, and IL2. The vaccine thus induced a significant immune response in mice. Mice vaccinated with mc2-CMX and challenged with M. tuberculosis showed better protection than mice immunized with wild-type M. smegmatis or BCG. To increase the safety and immunogenicity of the CMX antigens, we used a recombinant strain of M. smegmatis, IKE (immune killing evasion), to express CMX. The recombinant vaccine IKE-CMX induced a better protective response than mc2-CMX. The data presented here suggest that the expression of CMX antigens improves the immune response and the protection induced in mice when M. smegmatis is used as vaccine against tuberculosis. 相似文献
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Physical and functional interaction between p53 and the Werner's syndrome protein. 总被引:13,自引:0,他引:13
G Blander J Kipnis J F Leal C E Yu G D Schellenberg M Oren 《The Journal of biological chemistry》1999,274(41):29463-29469
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Cardoso AM Martins de Sousa E Viana-Niero C Bonfim de Bortoli F Pereira das Neves ZC Leão SC Junqueira-Kipnis AP Kipnis A 《Microbes and infection / Institut Pasteur》2008,10(14-15):1552-1557
A cluster of surgical site infection cases after arthroscopic and laparoscopic procedures occurred between 2005 and 2007 in Goiania, in the central region of Brazil. Nontuberculous mycobacteria (NTM) were isolated from samples (exudates from cutaneous abscesses) from 18 patients of seven private hospitals. There were no reports of post-surgical arthroscopic and laparoscopic mycobacterial infections in Goiania apart from this period. The 18 isolates were identified as Mycobacterium massiliense by PCR-restriction digestion of the hsp65 gene, pulsed-field gel electrophoresis (PFGE) comparisons, and rpoB partial gene sequencing. All isolates were typed as a single clone, indicating that they have the same origin, which suggests a common source of infection for all patients. 相似文献
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Xingliang Xu Claus Florian Stange Andreas Richter Wolfgang Wanek Yakov Kuzyakov 《Plant and Soil》2008,304(1-2):59-72
Effects of light on the short term competition for organic and inorganic nitrogen between maize and rhizosphere microorganisms
were investigated using a mixture of amino acid, ammonium and nitrate under controlled conditions. The amount and forms of
N added in the three treatments was identical, but only one of the three N forms was labeled with 15N. Glycine was additionally labeled with 14C to prove its uptake by maize and incorporation into microbial biomass in an intact form. Maize out-competed microorganisms
for during the whole experiment under low and high light intensity. Microbial uptake of 15N and 14C was not directly influenced by the light intensity, but was indirectly related to the impact the light intensity had on
the plant. More was recovered in microbial biomass than in plants in the initial 4 h under the two light intensities, although more 15N-glycine was incorporated into microbial biomass than in plants in the initial 4 h under low light intensity. Light had a
significant effect on uptake by maize, but no significant effects on the uptake of or 15N-glycine. High light intensity significantly increased plant uptake of and glycine 14C. Based on 14C to 15N recovery ratios of plants, intact glycine contributed at least 13% to glycine-derived nitrogen 4 h after tracer additions,
but it contributed only 0.5% to total nitrogen uptake. These findings suggest that light intensity alters the competitive
relationship between maize roots and rhizosphere microorganisms and that C4 cereals such as maize are able to access small
amounts of intact glycine. We conclude that roots were stronger competitor than microorganisms for inorganic N, but microorganisms
out competed plants during a short period for organic N, which was mineralized into inorganic N within a few hours of application
to the soil and was thereafter available for root uptake. 相似文献
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