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191.
Congcong Shen Anna Gunina Yu Luo Jianjun Wang Ji-Zheng He Yakov Kuzyakov Andreas Hemp Aimée T. Classen Yuan Ge 《Environmental microbiology》2020,22(8):3287-3301
Microbial elevational diversity patterns have been extensively studied, but their shaping mechanisms remain to be explored. Here, we examined soil bacterial and fungal diversity and community compositions across a 3.4 km elevational gradient (consists of five elevations) on Mt. Kilimanjaro located in East Africa. Bacteria and fungi had different diversity patterns across this extensive mountain gradient—bacterial diversity had a U shaped pattern while fungal diversity monotonically decreased. Random forest analysis revealed that pH (12.61% importance) was the most important factor affecting bacterial diversity, whereas mean annual temperature (9.84% importance) had the largest impact on fungal diversity, which was consistent with results obtained from mixed-effects model. Meanwhile, the diversity patterns and drivers of those diversity patterns differ among taxonomic groups (phyla/classes) within bacterial or fungal communities. Taken together, our study demonstrated that bacterial and fungal diversity and community composition responded differently to climate and edaphic properties along an extensive mountain gradient, and suggests that the elevational diversity patterns across microbial groups are determined by distinct environmental variables. These findings enhanced our understanding of the formation and maintenance of microbial diversity along elevation, as well as microbial responses to climate change in montane ecosystems. 相似文献
192.
Magorzata Klimko Krystyna Boratyska Jose Maria Montserrat Yakov Didukh Angel Romo Daniel Gmez Magdalena Kluza-Wieloch Katarzyna Marcysiak Adam Boratyski 《Flora》2007,202(2):133-147
The intra- and inter-population variation of Juniperus oxycedrus subsp. oxycedrus were examined biometrically on the basis of morphological characters of needles, seeds and cones. Cluster analysis of Euclidean distances and discriminant analysis were performed to verify the hypothesis of geographic differentiation and origin from the West- and East-Mediterranean Pleistocene refugial regions. The West-Mediterranean populations of J. oxycedrus subsp. oxycedrus differed from the eastern ones. The intra-populational differentiation of three of the West-Mediterranean populations was significantly higher than within the other West- and all the East-Mediterranean ones.Results confirm the hypothesis of probable different origins of the East- and West-Mediterranean populations of the taxon. 相似文献
193.
Nikolai Zlobin Konstantin Evlakov Yakov Alekseev Konstantin Blagodatskikh Aleksei Babakov Vasiliy Taranov 《Biochemistry and Biophysics Reports》2016
Plant cold shock domain proteins (CSDP) participate in maintenance of plant stress tolerance and in regulating their development. In the present paper we show that two out of three extremophyte plant Eutrema salsugineum proteins EsCSDP1-3, namely EsCSDP1 and EsCSDP3, possess high DNA-melting activity. DNA-melting activity of proteins was evaluated using molecular beacon assay in two ways: by measuring Tm parameter (the temperature at which half of the DNA beacon molecules is fully melted) and the beacon fluorescence at 4 °C. As the ratio protein/beacon was increased, a decrease in Tm was observed. Besides DNA-melting activity of full proteins, activity was measured for three isolated cold shock domains EsCSD1-3, C-terminal domain of EsCSDP1 (EsZnF1), as well as a mixture of EsCSD1 and EsZnF1. The Tm reduction efficiency of proteins formed the following sequence: EsCSDP3≈EsCSDP1>(EsCSD1+EsZnF1)>EsZnF1>EsCSDP2. Only full proteins EsCSDP3 and EsCSDP1 demonstrated DNA-melting activity at 4 °C. The presented experimental data indicate that i: interaction of EsCSDP1-3 with beacon single-stranded region is obligatory for efficient melting; ii: cold shock domain and C-terminal domain with zinc finger motifs should be present in one protein molecule to have high melting activity. 相似文献
194.
Zang Huadong Xiao Mouliang Wang Yidong Ling Ning Wu Jinshui Ge Tida Kuzyakov Yakov 《Plant and Soil》2019,439(1-2):113-130
Plant and Soil - Understanding the characteristics of root system is important for optimization of intercropping systems and increased utilization efficiency of limited resources. However, few... 相似文献
195.
General nonstationary birth–death process with possible catastrophes on finite state space is studied. The approach for obtaining the bounds on the rates of convergence to the limiting characteristics is outlined. Method for construction of the limiting characteristics is proposed. We also show that, as a rule, the initial conditions quickly become irrelevant. An example of respective process is shown in details. 相似文献