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561.
Ravichandran N. Murugan Jung-Eun Park Dan Lim Mija Ahn Chaejoon Cheong Taeho Kwon Ky-Youb Nam Sun Ho Choi Bo Yeon Kim Do-Young Yoon Michael B. Yaffe Dae-Yeul Yu Kyung S. Lee Jeong Kyu Bang 《Bioorganic & medicinal chemistry》2013,21(9):2623-2634
The polo-box domain (PBD) of polo-like kinase 1 (Plk1) is essentially required for the function of Plk1 in cell proliferation. The availability of the phosphopeptide-binding pocket on PBD provides a unique opportunity to develop novel protein–protein interaction inhibitors. Recent identification of a minimal 5-residue-long phosphopeptide, PLHSpT, as a Plk1 PBD-specific ligand has led to the development of several peptide-based inhibitors, but none of them is cyclic peptide. Through the combination of single-peptoid mimics and thio-ether bridged cyclization, we successfully demonstrated for the first time two cyclic peptomers, PL-116 and PL-120, dramatically improved the binding affinity without losing mono-specificity against Plk1 PBD in comparison with the linear parental peptide, PLHSpT. These cyclic peptomers could serve as promising templates for future drug designs to inhibit Plk1 PBD. 相似文献
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Wirth MC Zaritsky A Ben-Dov E Manasherob R Khasdan V Boussiba S Walton WE 《Environmental microbiology》2007,9(6):1393-1401
Sixteen Escherichia coli clones were assayed against susceptible and Bacillus thuringiensis-resistant Culex quinquefasciatus larvae. The clones expressed different combinations of four genes from Bacillus thuringiensis ssp. israelensis; three genes encoded mosquitocidal toxins (Cry11Aa, Cry4Aa and Cyt1Aa) and the fourth encoded an accessory protein (P20). The cross-resistance spectra of the mosquitoes were similar to the profiles for recombinant B. thuringiensis strains expressing B. thuringiensis toxin genes, but with varied toxicity levels. The toxicity of the recombinants towards resistant mosquito larvae was improved when p20 and cyt1Aa were expressed in combination with cry4Aa and/or cry11Aa. Recombinant pVE4-ADRC, expressing cry4Aa, cry11Aa, p20 and cyt1Aa, was the most active against the resistant Culex, and resistance levels did not exceed fourfold. These results indicate that B. thuringiensis ssp. israelensis genes expressed in a heterologous host such as E. coli can be effective against susceptible and B. thuringiensis-resistant larvae and suppress resistance. 相似文献
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P W Catron E T Flynn L Yaffe M E Bradley L B Thomas D Hinman S Survanshi J T Johnson J Harrington 《Journal of applied physiology (Bethesda, Md. : 1985)》1984,57(2):467-474
The lung's response to decompression was studied in dogs anesthetized with pentobarbital sodium. Arterial pressure, hematocrit, right ventricular pressure, left ventricular end-diastolic pressure (LVEDP), dynamic compliance (CL), pulmonary resistance (RL), and arterial PO2, PCO2, and pH were measured prior to and for 3 h after a simulated air dive to 300 feet of seawater. Bronchoscopy was performed predive and at 3 h postdive. At 3 h animals were killed, and sections of lung were excised for histological examination. The decompression profile used regularly produced pulmonary hypertension, systemic hypotension, hemoconcentration, and arterial hypoxemia. CL fell in all but one dived animal. RL was more variable but remained unchanged postdive in most animals. The decompression stress did not alter the bronchoscopic and histological appearance of the airway mucosa. Pulmonary edema was regularly observed in histological sections and occurred without elevations of LVEDP. We concluded that noncardiac pulmonary edema is the principal response of the lung to decompression stress. 相似文献
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A column that specifically binds gibberellins was prepared bycovalent binding of anti-GA antibodies to Sepharose. By usingsuch an immunoadsorbent column, radio-immunoassay for quantitativedetermination of gibberellins was developed. Using this method,diffusible GA from apices of etiolated pea seedlings was isolatedin one step and determined quantitatively. (Received March 29, 1974; ) 相似文献
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