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101.
Abstract: Diapause induction and photoperiodic response curves were determined for 33 strains of Tetranychus pueraricola derived from kudzu vine at three constant temperatures (15, 18 and 20°C) under a short-day condition (10 : 14 h; light : dark). Females of all but one of the strains entered diapause at all three temperatures with little variation in diapause percentages among the strains. The exception was the southernmost strain, which was found to be a non-diapause (ND) strain. The critical photoperiod gradually decreased towards the south at a rate of about 1 h for each 5 degrees of latitude. The diapause strains (D1 and D2) exhibited 100% diapause, whereas the ND strain exhibited 0% diapause. By crossing these strains, we determined that 'non-diapause' was a dominant character over 'diapause' and the character was controlled by simple Mendelian inheritance. To clarify why the female progeny from the crosses between the D1 and ND strains did not segregate into the diapause and non-diapause phenotypes in a 1:1 ratio in the B1 generation, round-robin crosses were carried out among the three strains. The results showed that the F1 generation was reproductively compatible and showed high egg hatchability with a female-biased sex ratio. In the B1 generation, the crosses between the D1 and ND strains and between the D1 and D2 strains exhibited extremely low egg hatchability and produced mostly female progeny, whereas offspring from the crosses between the D2 and ND strains showed more than 50% hatchability for B1 eggs and a female-biased sex ratio. Thus, the absence of segregation observed in the crosses between the D1 and ND strains appears to be due to the severe hybrid breakdown that occurred in the B1 generation. 相似文献
102.
103.
H Ishida Y Kuruta O Gotoh C Yamashita Y Yoshida M Noshiro 《Journal of biochemistry》1999,126(1):19-25
The rat CYP8B cDNA encoding sterol 12alpha-hydroxylase was cloned and sequenced. The amino acid sequence of the heme-binding region of CYP8B was close to those of CYP7A (cholesterol 7alpha-hydroxylase) and CYP7B (oxysterol 7alpha-hydroxylase). Molecular phylogenetic analysis suggests that CYP8B and the CYP7 family derive from a common ancestor. The P450s of the CYP7 and CYP8 families, except for CYP8A (prostacyclin synthase), catalyze the oxygenation of sterols from an alpha surface in the middle of the steroid skeleton. These facts suggest that CYP8B is a P450 closely linked to those of the CYP7 family. CYP8B was expressed specifically in liver. Hepatic CYP8B mRNA level and the 12alpha-hydroxylase activity were altered by cholestyramine feeding, starvation, streptozotocin-induced diabetes mellitus, and administration of clofibrate, dexamethasone or thyroxin, indicating the pretranslational regulation of CYP8B expression. The enhanced CYP8B mRNA expression in streptozotocin-induced diabetic rats was significantly decreased by insulin within 3 h of its administration. These facts demonstrate a regulatory role of insulin in CYP8B expression as a suppressor. 相似文献
104.
De Novo Synthesis of Sphingolipids Is Required for Cell Survival by Down-Regulating c-Jun N-Terminal Kinase in Drosophila Imaginal Discs 下载免费PDF全文
105.
CCAAT/enhancer-binding protein homologous protein (CHOP) regulates osteoblast differentiation 总被引:4,自引:0,他引:4 下载免费PDF全文
106.
Tomoki Kozawa Kenichiro Sugitani Dorothy Z. Oehler Christopher H. House Izumi Saito Takeshi Watanabe Toshiyuki Gotoh 《Geobiology》2019,17(2):113-126
Lenticular, and commonly flanged, microfossils in 3.0–3.4 Ga sedimentary deposits in Western Australia and South Africa are unusually large (20–80 μm across), robust, and widespread in space and time. To gain insight into the ecology of these organisms, we performed simulations of fluid dynamics of virtual cells mimicking lenticular forms of variable sizes, oblateness, flange presence, and flange thickness. Results demonstrate that (a) the flange reduces sedimentation velocity, (b) this flange function works more effectively in larger cells, and (c) modest oblateness lowers sedimentation rate. These observations support interpretations that the lenticular microbes were planktonic—a lifestyle that could have been advantageous in an early Earth harsh environment including violent volcanic activities, repeated asteroid impacts, and relatively high UV‐radiation. Although the robustness of these organisms could have provided additional protection on the early Earth, this architecture may have impeded a planktonic lifestyle by increasing cell density. However, our data suggest that this disadvantage could have been compensated by enlargement of cell volume, which could have enhanced the ability of the flange to slow sedimentation rate, especially if coupled with vacuolation. The results of this simulation study may help to explain the unique morphology and unusually large size of these Archean microfossils. 相似文献
107.
108.
Bacteriophages (or phages) play major roles in the evolution of bacterial pathogens via horizontal gene transfer. Multiple phages are often integrated in a host chromosome as prophages, not only carrying various novel virulence-related genetic determinants into host bacteria but also providing various possibilities for prophage-prophage interactions in bacterial cells. In particular, Escherichia coli strains such as Shiga toxin (Stx)-producing E. coli (STEC) and enteropathogenic E. coli (EPEC) strains have acquired more than 10 prophages (up to 21 prophages), many of which encode type III secretion system (T3SS) effector gene clusters. In these strains, some prophages are present at a single locus in tandem, which is usually interpreted as the integration of phages that use the same attachment (att) sequence. Here, we present phages integrating into T3SS effector gene cluster-associated loci in prophages, which are widely distributed in STEC and EPEC. Some of the phages integrated into prophages are Stx-encoding phages (Stx phages) and have induced the duplication of Stx phages in a single cell. The identified attB sequences in prophage genomes are apparently derived from host chromosomes. In addition, two or three different attB sequences are present in some prophages, which results in the generation of prophage clusters in various complex configurations. These phages integrating into prophages represent a medically and biologically important type of inter-phage interaction that promotes the accumulation of T3SS effector genes in STEC and EPEC, the duplication of Stx phages in STEC, and the conversion of EPEC to STEC and that may be distributed in other types of E. coli strains as well as other prophage-rich bacterial species. 相似文献
109.
Hirotada Akiyama Aina Gotoh Ryong-Woon Shin Tomoe Koga Tsubasa Ohashi Wataru Sakamoto Akihiro Harada Hiroyuki Arai Akira Sawa Chiyoko Uchida Takafumi Uchida 《The Journal of biological chemistry》2009,284(47):32695-32699
Here, we report a novel role for hGas7b (human growth arrest specific protein 7b) in the regulation of microtubules. Using a bioinformatic approach, we studied the actin-binding protein hGas7b with a structural similarity to the WW domain of a peptidyl prolyl cis/trans isomerase, Pin1, that facilitates microtubule assembly. Thus, we have demonstrated that hGas7b binds Tau at the WW motif and that the hGas7b/Tau protein complex interacts with the microtubules, promoting tubulin polymerization. Tau, in turn, contributes to protein stability of hGas7b. Furthermore, we observed decreased levels of hGas7b in the brains from patients with Alzheimer disease. These results suggest an important role for hGas7b in microtubular maintenance and possible implication in Alzheimer disease. 相似文献
110.
The aim of this study is to develop a prototype system for noncontact, noninvasive and unconstrained vital sign monitoring using microwave radar and to use the system to measure the respiratory rate of a Japanese black bear (Ursus thibetanus japonicus) during hibernation for ensuring the bear's safety. Ueno Zoological Gardens in Tokyo planned to help the Japanese black bear (female, approximately 2 years of age) going into hibernation. The prototype system has a microwave Doppler radar antenna (10-GHz frequency, approximately 7 mW output power) for measuring motion of the body surface caused by respiratory activity without making contact with the body. Monitoring using this system was conducted from December 2006 to April 2007. As a result, from December 18, 2006, to March 17, 2007, similar behaviors reported by earlier studies were observed, such as sleeping with curled up posture and not eating, urinating or defecating. During this hibernation period and also around the time of hibernation, the prototype system continuously measured cyclic oscillations. The presence of cyclic vibrations at 8-sec intervals (about 7 bpm) was confirmed by the system before she entered hibernation on December 3, 2006. The respiratory rate gradually decreased, and during the hibernation period the respiratory rate was extremely low at approximately 2 bpm with almost no change. The results show that motion on the body surface caused by respiratory activity can be measured without touching the animal's body. Thus, the microwave radar employed here can be utilized as an aid in observing vital signs of animals. Zoo Biol 28:259–270, 2009. © 2009 Wiley-Liss, Inc. 相似文献