全文获取类型
收费全文 | 1722篇 |
免费 | 71篇 |
国内免费 | 3篇 |
出版年
2023年 | 21篇 |
2022年 | 46篇 |
2021年 | 77篇 |
2020年 | 56篇 |
2019年 | 50篇 |
2018年 | 73篇 |
2017年 | 59篇 |
2016年 | 92篇 |
2015年 | 92篇 |
2014年 | 109篇 |
2013年 | 133篇 |
2012年 | 167篇 |
2011年 | 146篇 |
2010年 | 90篇 |
2009年 | 69篇 |
2008年 | 65篇 |
2007年 | 49篇 |
2006年 | 40篇 |
2005年 | 49篇 |
2004年 | 34篇 |
2003年 | 37篇 |
2002年 | 27篇 |
2001年 | 24篇 |
2000年 | 23篇 |
1999年 | 14篇 |
1998年 | 9篇 |
1997年 | 6篇 |
1996年 | 6篇 |
1995年 | 4篇 |
1994年 | 10篇 |
1993年 | 7篇 |
1992年 | 14篇 |
1991年 | 11篇 |
1990年 | 8篇 |
1989年 | 6篇 |
1988年 | 7篇 |
1986年 | 8篇 |
1985年 | 6篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1982年 | 5篇 |
1981年 | 4篇 |
1980年 | 4篇 |
1978年 | 5篇 |
1977年 | 4篇 |
1976年 | 4篇 |
1975年 | 4篇 |
1973年 | 2篇 |
1972年 | 6篇 |
1971年 | 2篇 |
排序方式: 共有1796条查询结果,搜索用时 15 毫秒
131.
AFD-Net: Apple Foliar Disease multi classification using deep learning on plant pathology dataset 总被引:2,自引:0,他引:2
Yadav Anju Thakur Udit Saxena Rahul Pal Vipin Bhateja Vikrant Lin Jerry Chun-Wei 《Plant and Soil》2022,477(1-2):595-611
Plant and Soil - Plant diseases significantly affect the crop, so their identification is very important. Correct identification of these diseases is crucial for establishing a good disease control... 相似文献
132.
Singh Awtar Bali Aradhana Kumar Ashwani Yadav Rajender Kumar Minhas Paramjit Singh 《Journal of Plant Growth Regulation》2022,41(5):1989-1998
Journal of Plant Growth Regulation - Exogenous applications of plant growth regulators (PGRs) were evaluated for their effects on growth, physiological attributes, and yield of sodicity stressed... 相似文献
133.
Bego?a Carrasco Tribhuwan Yadav Ester Serrano Juan C. Alonso 《Nucleic acids research》2015,43(12):5984-5997
Genetic data have revealed that the absence of Bacillus subtilis RecO and one of the end-processing avenues (AddAB or RecJ) renders cells as sensitive to DNA damaging agents as the null recA, suggesting that both end-resection pathways require RecO for recombination. RecA, in the rATP·Mg2+ bound form (RecA·ATP), is inactive to catalyze DNA recombination between linear double-stranded (ds) DNA and naked complementary circular single-stranded (ss) DNA. We showed that RecA·ATP could not nucleate and/or polymerize on SsbA·ssDNA or SsbB·ssDNA complexes. RecA·ATP nucleates and polymerizes on RecO·ssDNA·SsbA complexes more efficiently than on RecO·ssDNA·SsbB complexes. Limiting SsbA concentrations were sufficient to stimulate RecA·ATP assembly on the RecO·ssDNA·SsbB complexes. RecO and SsbA are necessary and sufficient to ‘activate’ RecA·ATP to catalyze DNA strand exchange, whereas the AddAB complex, RecO alone or in concert with SsbB was not sufficient. In presence of AddAB, RecO and SsbA are still necessary for efficient RecA·ATP-mediated three-strand exchange recombination. Based on genetic and biochemical data, we proposed that SsbA and RecO (or SsbA, RecO and RecR in vivo) are crucial for RecA activation for both, AddAB and RecJ–RecQ (RecS) recombinational repair pathways. 相似文献
134.
Pravin Patil Surendra Kumar Malik Shrikant Sutar Shrirang Yadav Joseph John Kangila Venkataraman Bhat 《Nordic Journal of Botany》2015,33(6):696-707
Seed morphology of Abelmoschus is known to be variable, but patterns of variation have never been critically studied. We studied seed macro‐ and micro‐morphological characters, including seed shape/size, seed coat pattern and trichome density/structure in multiple samples to evaluate the taxonomic significance of seed characters. Among the studied characters, seed shape and trichome structure were found to have major taxonomic importance and proved to be valuable characters for separating taxa. Two main seed types were present: seeds with deciduous trichomes and seeds with persistent trichomes. These characters offer significant evidence to the distinctness of certain species (A. esculentus, A. moschatus subsp. moschatus, A. moschatus subsp. tuberosus, A. crinitus and A. angulosus). Further, our results indicate that A. moschatus subsp. tuberosus should be maintained as a separate subspecies while A. manihot subsp. tetraphyllus var. pungens may be merged in A. angulosus. No significant intraspecific variation was observed, except in A. esculentus. We conclude that seed coat sculpturing and seed trichomes do indeed provide stable and diagnostic characters for many morphologically closely related taxa of Abelmoschus and that LM/SEM techniques can be useful in solving systematic problems and management of Abelmoschus genetic resources. 相似文献
135.
136.
137.
Swati Agarwal Shashi Kant Tiwari Brashket Seth Anuradha Yadav Anshuman Singh Anubha Mudawal Lalit Kumar Singh Chauhan Shailendra Kumar Gupta Vinay Choubey Anurag Tripathi Amit Kumar Ratan Singh Ray Shubha Shukla Devendra Parmar Rajnish Kumar Chaturvedi 《The Journal of biological chemistry》2015,290(34):21163-21184
The human health hazards related to persisting use of bisphenol-A (BPA) are well documented. BPA-induced neurotoxicity occurs with the generation of oxidative stress, neurodegeneration, and cognitive dysfunctions. However, the cellular and molecular mechanism(s) of the effects of BPA on autophagy and association with oxidative stress and apoptosis are still elusive. We observed that BPA exposure during the early postnatal period enhanced the expression and the levels of autophagy genes/proteins. BPA treatment in the presence of bafilomycin A1 increased the levels of LC3-II and SQSTM1 and also potentiated GFP-LC3 puncta index in GFP-LC3-transfected hippocampal neural stem cell-derived neurons. BPA-induced generation of reactive oxygen species and apoptosis were mitigated by a pharmacological activator of autophagy (rapamycin). Pharmacological (wortmannin and bafilomycin A1) and genetic (beclin siRNA) inhibition of autophagy aggravated BPA neurotoxicity. Activation of autophagy against BPA resulted in intracellular energy sensor AMP kinase (AMPK) activation, increased phosphorylation of raptor and acetyl-CoA carboxylase, and decreased phosphorylation of ULK1 (Ser-757), and silencing of AMPK exacerbated BPA neurotoxicity. Conversely, BPA exposure down-regulated the mammalian target of rapamycin (mTOR) pathway by phosphorylation of raptor as a transient cell''s compensatory mechanism to preserve cellular energy pool. Moreover, silencing of mTOR enhanced autophagy, which further alleviated BPA-induced reactive oxygen species generation and apoptosis. BPA-mediated neurotoxicity also resulted in mitochondrial loss, bioenergetic deficits, and increased PARKIN mitochondrial translocation, suggesting enhanced mitophagy. These results suggest implication of autophagy against BPA-mediated neurodegeneration through involvement of AMPK and mTOR pathways. Hence, autophagy, which arbitrates cell survival and demise during stress conditions, requires further assessment to be established as a biomarker of xenoestrogen exposure. 相似文献
138.
139.
Avnish Kumar Monika Asthana Poonam Gupta Shweta Yadav Deepti Sharma Km Neeraj Singh Sunil Kumar 《Bioinformation》2015,11(1):1-5
Dye׳s residues in textile effluents are hazardous for humans and animals health. Such pollutants can be degraded into
non-harmful molecules using biological approaches that are considered cheaper and ecologically safer. Isolated 15 bacterial
cultures from soil that could be used in biological system were showed decolorization capacity for Acid Green dye (33.9% to
94.0%) using thin layer chromatography and broth culture method. The most promising cultures (AMC3) to decolorize
Acid green Dye (94.6%) was re-coded as NSDSUAM for submitting at IMTECH, Chandigarh for sequencing. The 16SrRNA
sequencing suggested that it can be a variant of Pseudomonas geniculata (99.85% identical similarity) with difference of 2 base pairs
to reference strain Pseudomonas geniculata ATCC 19374(T). Thus present study proposed dye decolorizing efficiency of the
isolated strain of Pseudomonas geniculata that was previously unnoticed. The sequence is deposited in NCBI GenBank with the
accession number . KP238100相似文献