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81.
Yumei Yang Yanjuan Liu Hang Yuan Xian Liu Yanxiu Gao Ming Gong 《Molecular membrane biology》2016,33(3-5):39-50
Membrane-bound pyrophosphatases (PPases) are involved in the adaption of organisms to stress conditions, which was substantiated by numerous plant transgenic studies with H+-PPase yet devoid of any correlated evidences for other two subfamilies, Na+-PPase and Na+,H+-PPase. Herein, we demonstrate the gene cloning and functional evaluation of the membrane-bound PPase (CmPP) of the human gut microbe Clostridium methylpentosum. The CmPP gene encodes a single polypeptide of 699 amino acids that was predicted as a multi-spanning membrane and K+-dependent Na+,H+-PPase. Heterologous expression of CmPP could significantly enhance the salt tolerance of both Escherichia coli and Saccharomyces cerevisiae, and this effect in yeast could be fortified by N-terminal addition of a vacuole-targeting signal peptide from the H+-PPase of Trypanosoma cruzi. Furthermore, introduction of CmPP could remarkably improve the salt tolerance of tobacco, implying its potential use in constructing salt-resistant transgenic crops. Consequently, the possible mechanisms of CmPP to underlie salt tolerance are discussed. 相似文献
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Jing Chen Cheng-Shi Jiang Wen-Quan Ma Li-Xin Gao Jing-Xu Gong Jing-Ya Li Jia Li Yue-Wei Guo 《Bioorganic & medicinal chemistry letters》2013,23(18):5061-5065
Bruguiesulfurol (1), a cyclic 4-hydroxy-dithiosulfonate isolated from mangrove plant Bruguiera gymnorrhiza, was concisely synthesized for the first time in four steps, and a series of its synthetic derivatives were evaluated for in vitro inhibitory effects on PTP1B and related PTPs. Some derivatives were found to have improved pharmacological profile compared with hit 1. Among them, 5a showed the potent selectivity towards PTP1B over other PTPs, including TCPTP, and 7j exhibited the strongest PTP1B inhibitory activity with an IC50 value of 4.54 μM. 相似文献
84.
Jiao P Cao L Yuan R Wei L Song Y Shen D Gong L Luo K Ren T Liao M 《Journal of virology》2012,86(14):7716
An H10N8 avian influenza virus (AIV), designated A/Duck/Guangdong/E1/2012 (H10N8), was isolated from a duck in January 2012. This is first report that this subtype of AIV was isolated from a live bird market (LBM) in Guangdong Province in southern China. Furthermore, the complete genome of this strain was analyzed. The availability of genome sequences is helpful to further investigations of epidemiology and molecular characteristics of AIV in southern China. 相似文献
85.
采用肾乳头暴露方法活体观察Sprague-Dawley大鼠肾髓质微循环。结果发现:正常成年大鼠肾乳头可暴露1.1±0.5mm; 乳头表面直血管数29.8±6.3;升、降支比例3.4:1。升支平均直径13.68±6.13μm,降支10.8±2.57μm。肾乳头连续暴露观察10h,其微循环未发生明显病理性改变。说明这一方法可以用于肾髓质微循环活体研究。 相似文献
86.
中国"三江并流"纵谷地蚤类丰富度与区系沿纬度梯度的水平分布格局 总被引:9,自引:0,他引:9
为探讨横断山区蚤类物种丰富度与区系沿纬度梯度变化的基本规律以及影响它们分布的主要生态因子,作者就云南西部横断山区21o-28oN的8个纬度梯度带目前所知分布的9科45属153种(亚种)蚤类的水平分布资料进行了综合整理和统计分析。结果表明:(1)这一区域蚤类科、属、种丰富度,特有种丰富度和特有度,以及东洋界和古北界区系成分物种丰富度沿纬度梯度的变化,都呈现了随纬度增加先增高后降低的单峰分布格局,最大峰值出现在25o-27oN之间,研究认为,形成物种密度高峰的主要因素是两大区系分界线和交错区的边缘效应;(2)东洋和古北两大区系成分构成比的水平分布格局截然不同,前者随纬度的增加递减,后者则随纬度的增加呈递增的趋势,两区系成分过渡或交错区的跨度在23o-29oN之间,并在25o-27oN形成交汇和分异的中心;(3)聚类分析结果表明,横断山8个纬度梯度带的蚤类总体上归为3个主要地域区系类型,反映出纬度、地理气候环境等因素对蚤类区系及物种分布的影响,以及蚤类区系和物种组成沿纬度梯度的水平分布格局与地理和气候环境的统一性;(4)蚤类的属、种β多样性沿纬度梯度的水平分布基本呈现双峰格局,峰值反映出蚤类的组成及分布在不同纬度梯度、气候带之间的过渡与转变,说明β多样性的水平分布格局与纬度梯度的气候、环境变化程度的关系密切,以及过渡区的边缘效应对两区系物种丰富度高峰的形成具有重要影响;(5)横断山区蚤类物种丰富度与区系的水平分布与垂直分布的规律和格局基本相同,显示了具有同源性的水平地带性与垂直地带性对蚤类空间分布格局所产生的类似影响,证实了横断山区蚤类物种丰富度与区系的水平分布格局理应出现近似于它们垂直分布的一般规律与格局的推论;(6)横断山25o-27oN的区域形成了两大区系物种交汇和分异的中心,由于边缘效应和复杂的地理景观,这里蚤类科、属、种、特有种都具有较高多样性,据此作者推断,这里可能是我国横断山区多物种保存、分布和分化的核心区。 相似文献
87.
The living morphology, infraciliature and morphogenesis of a new marine cyrtophorid ciliate, Hartmannula sinica nov. spec., collected from Qingdao, north China, have been investigated. The new species is characterized by: size in vivo 90–130×40–50 μm, body long elliptical in outline, cilium-free field covered with a conspicuous alveolar layer; 24–31 ventral kineties, the rightmost 6–9 of which extend apically; 20–24 nematodesmal rods; about 15 sparsely distributed contractile vacuoles; a yellowish pigment spot is always present near the anterior tip of the cell. Morphogenetic events exhibit a pattern, which is homologous with that of related cyrtophorids. The main features are as follows: (1) preoral and circumoral kineties of opisthe develop from the oral primordium that forms in mid-body from sections of 3 postoral kineties, while parental preoral and circumoral kineties are retained; (2) parental cytostome and nematodesmal rods are resorbed in middle divisional stages and then replaced by newly formed structures; (3) the heteromerous macronucleus unifies in the late divisional stage. 相似文献
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90.
Thomas R. Shelite Yuejin Liang Hui Wang Nicole L. Mendell Brandon J. Trent Jiaren Sun Bin Gong Guang Xu Haitao Hu Donald H. Bouyer Lynn Soong 《PLoS neglected tropical diseases》2016,10(3)
Endothelial cells (EC) are the main target for Orientia tsutsugamushi infection and EC dysfunction is a hallmark of severe scrub typhus in patients. However, the molecular basis of EC dysfunction and its impact on infection outcome are poorly understood. We found that C57BL/6 mice that received a lethal dose of O. tsutsugamushi Karp strain had a significant increase in the expression of IL-33 and its receptor ST2L in the kidneys and liver, but a rapid reduction of IL-33 in the lungs. We also found exacerbated EC stress and activation in the kidneys of infected mice, as evidenced by elevated angiopoietin (Ang) 2/Ang1 ratio, increased endothelin 1 (ET-1) and endothelial nitric oxide synthase (eNOS) expression. Such responses were significantly attenuated in the IL-33-/- mice. Importantly, IL-33-/- mice also had markedly attenuated disease due to reduced EC stress and cellular apoptosis. To confirm the biological role of IL-33, we challenged wild-type (WT) mice with a sub-lethal dose of O. tsutsugamushi and gave mice recombinant IL-33 (rIL-33) every 2 days for 10 days. Exogenous IL-33 significantly increased disease severity and lethality, which correlated with increased EC stress and activation, increased CXCL1 and CXCL2 chemokines, but decreased anti-apoptotic gene BCL-2 in the kidneys. To further examine the role of EC stress, we infected human umbilical vein endothelial cells (HUVEC) in vitro. We found an infection dose-dependent increase in the expression of IL-33, ST2L soluble ST2 (sST2), and the Ang2/Ang1 ratio at 24 and 48 hours post-infection. This study indicates a pathogenic role of alarmin IL-33 in a murine model of scrub typhus and highlights infection-triggered EC damage and IL-33-mediated pathological changes during the course of Orientia infection. 相似文献