百草枯中毒大鼠TNF-a、IL-10的表达及大黄保护作用的研究

目的：观察大黄对急性百草枯中毒大鼠TNF-α、IL-10的干预作用,探讨其可能的作用机制。方法：90只SD大鼠随机分为生理盐水对照组（A组）、PQ（60 mg/kg）灌胃染毒组（B组）、生大黄（300mg/kg.d）干预组（C组）,每组30只。中毒后6h、24h、72h分批处死存活的大鼠,并且检测大鼠血浆TNF-α、IL-10水平。结果：B组、C组TNF-α、IL-10水平在染毒后6h开始升高,72h达到高峰,与A组相比,差异有统计学意义（P〈0.05、P〈0.01）,在相同时间点C组TNF-α和IL-10的表达低于B组,差异均有统计学意义（P〈0.01）。B组、C组血浆TNF-α、IL-10水平与中毒时间呈显著正性相关关系（r=0.849,P〈0.01;r=0.790,P〈0.01;r=0.0.943,P〈0.01;r=0.892,P〈0.01）。结论：大黄能够通过降低百草枯中毒大鼠体内的TNF-α、IL-10水平,减轻百草枯对大鼠的损伤作用     

High major histocompatibility complex-unrestricted lysis of simian immunodeficiency virus envelope-expressing cells predisposes macaques to rapid AIDS progression

Before the development of virus-specific immune responses, peripheral blood mononuclear cells (PBMC) from uninfected rhesus monkeys and human beings have the capacity to lyse target cells expressing simian immunodeficiency virus (SIV) or human immunodeficiency virus-1 (HIV) envelope (gp130 and gp120) antigens. Lysis by naive effector cells does not require major histocompatibility complex (MHC)-restricted antigen presentation, is equally effective for allogeneic and xenogeneic targets, and is designated MHC-unrestricted (UR) lysis. UR lysis is not sensitive to EGTA and does not require de novo RNA or protein synthesis. Several kinds of envelope-expressing targets, including cells that poorly express MHC class I antigens, can be lysed. CD4(+) effectors are responsible for most of the lytic activity. High lysis is correlated with high expression of HIV or SIV envelope, specifically, the central one-third of the gp130 molecule, and lysis is completely inhibited by a monoclonal antibody against envelope. Our work extends observations of human lymphocytes expressing HIV gp120 to the SIV/rhesus monkey model for AIDS. Additionally, we address the relevance of UR lysis in vivo. A survey of PBMC from 56 uninfected rhesus monkeys indicates that 59% of the individuals had peak UR lytic activity above 15% specific lysis. Eleven of these monkeys were subsequently infected with SIV. Animals with UR lytic activity above 15% specific lysis were predisposed to more rapid disease progression than animals with low UR lytic activity, suggesting a strong correlation between this form of innate immunity and disease progression to AIDS.     

PKA modulates GSK-3beta- and cdk5-catalyzed phosphorylation of tau in site- and kinase-specific manners

Phosphorylation of tau protein is regulated by several kinases, especially glycogen synthase kinase 3beta (GSK-3beta), cyclin-dependent protein kinase 5 (cdk5) and cAMP-dependent protein kinase (PKA). Phosphorylation of tau by PKA primes it for phosphorylation by GSK-3beta, but the site-specific modulation of GSK-3beta-catalyzed tau phosphorylation by the prephosphorylation has not been well investigated. Here, we found that prephosphorylation by PKA promotes GSK-3beta-catalyzed tau phosphorylation at Thr181, Ser199, Ser202, Thr205, Thr217, Thr231, Ser396 and Ser422, but inhibits its phosphorylation at Thr212 and Ser404. In contrast, the prephosphorylation had no significant effect on its subsequent phosphorylation by cdk5 at Thr181, Ser199, Thr205, Thr231 and Ser422; inhibited it at Ser202, Thr212, Thr217 and Ser404; and slightly promoted it at Ser396. These studies reveal the nature of the inter-regulation of tau phosphorylation by the three major tau kinases.     

鸡减蛋综合征病毒(EDSV)基因组右末端结构的分析

从中国发病鸡中分离的鸡减蛋综合征病毒（ＥｇｇＤｒｏｐＳｙｎｄｒｏｍＶｉｒｕｓ,ＥＤＳＶ）弱毒株（ＡＡ－２）,其基因组全长约为３３ｋｂ。用限制性内切酶ＨｉｎｄⅢ水解ＥＤＳＶ全基因组,构建了以ｐＢｌｕｅｓｃｒｉｐｔⅡ（ＫＳ＋）为载体的右末端片段的克隆（约４．２ｋｂ）,对其进行了序列测定和结构分析。该片段全长４１８３个碱基对（ｂｐ）,位于基因组右末端８７．３ｍ．ｕ．－１００ｍ．ｕ．。结果显示,该片段与哺乳动物腺病毒右末端Ｅ４区结构不同,与禽Ⅰ型腺病毒代表株ＣＥＬＯ右末端片段亦无同源性。本文为深入了解ＥＤＳＶ基因组结构特点,ＥＤＳＶ与其他腺病毒基因结构与功能的进化关系和ＥＤＳＶ载体的构建奠定了分子生物学基础     

新疆石河子地区印度麻花叶病植株病毒分离物的鉴定

1984年,从新疆石河子农学院实验站印度麻花叶病植株上,分离到一株病毒分离物Sc-1,经汁液摩擦接种试验表明,它可以侵染10种豆科植物和2种藜科植物。在印度麻、蚕豆、豌豆、箭舌豌豆、扁豆、山藜豆、田菁和红三叶草上引起系统花叶,在豇豆上产生局部枯斑和系统花叶,在苋色藜、昆诺藜上表现为系统黄斑。失毒温度为55～60℃,稀释限点10~(-3)～10~(-4),体外保毒期3～4天。可经汁液和蚜虫传播,不通过种子传毒。病毒粒体为线条状,大小为13～15×750nm。光学显微镜检查可见,病叶表皮细胞内形成不定形的内含体。电镜下可见风轮状、环状内含体。分离物Sc-1与菜豆黄色花叶病毒(BYMV)抗血清呈阳性反应。我们将Sc-1归为菜豆黄色花叶病毒(BYMV),且为豇豆株系。     

合成生物制造2022

本文对2022年《生物工程学报》发表的与合成生物制造相关的综述和研究论文进行了评述,重点讨论了DNA测序、DNA合成、DNA编辑、基因表达调控和数学细胞模型等底层技术,酶的设计、改造和应用技术,化学品生物催化、氨基酸及其衍生物、有机酸、天然化合物、抗生素与活性肽、功能多糖、功能蛋白质等重要产品的生物制造技术,一碳化合物和生物质原料利用技术以及合成微生物组技术,以帮助读者从一个侧面了解合成生物制造相关技术和产业的发展情况。