Feng Zhou  Fan Lu  Can Zhang  He‐Xing Qi  Xiao‐Dong Wang  Guo‐Zhen Zhang 《Journal of Phytopathology》2017,165(7-8):455-462

This study assessed the fenhexamid sensitivity of 143 Botrytis cinerea isolates collected from greenhouse strawberries in five regions of China between 2012 and 2013, and identified four isolates with moderate levels of resistance: two from the Xinjiang Uygur Autonomous Region and two from Hebei Province. The baseline fenhexamid sensitivity of B. cinerea exhibited a unimodal distribution with a mean EC₅₀ value of 0.20 ± 0.10 μg/ml (SD). The EC₅₀ values of the fenhexamid‐resistant isolates ranged from 0.05 to 0.40 μg/ml. Molecular analysis of the fenhexamid target gene erg27 revealed that the resistant isolates collected from Xinjiang (163‐6 and 163‐22) contained three mutations that led to amino acid changes (V365A, E368D and A378T) known to be associated with fenhexamid resistance, but that the isolates from Hebei lacked any mutations, indicating that an alternative mechanism could be responsible for their resistance. Most of the biological characteristics of the fenhexamid‐resistant isolates, such as mycelial growth, sclerotia production and pathogenicity, did not significantly differ from those of the sensitive ones (p ≤ .05), but it was noted that some of the resistant isolates exhibited reduced rates of sporulation and spore germination. In addition, the resistant isolates exhibited lower osmotic sensitivity than the sensitive ones. The study found no evidence of cross‐resistance with other fungicides, but that there was negative cross‐resistance with procymidone, iprodione, carbendazim and pyraclostrobin, which indicates that the inclusion of these fungicides within an integrated pest management (IPM) programme could help to minimize the risk of fenhexamid resistance developing in B. cinerea.  相似文献   

    

Xu Chen  Bin Liu  Cheng Zhong  Zhi Liu  Jie Liu  Lu Ma  Yida Deng  Xiaopeng Han  Tianpin Wu  Wenbin Hu  Jun Lu 《Liver Transplantation》2017,7(18)

A facile and binder‐free method is developed for the in situ and horizontal growth of ultrathin mesoporous Co₃O₄ layers on the surface of carbon fibers in the carbon cloth (ultrathin Co₃O₄/CC) as high‐performance air electrode for the flexible Zn–air battery. In particular, the ultrathin Co₃O₄ layers have a maximum contact area on the conductive support, facilitating the rapid electron transport and preventing the aggregation of ultrathin layers. The ultrathin feature of Co₃O₄ layers is characterized by the transmission electron microscopy, Raman spectra, and X‐ray absorption fine structure spectroscopy. Benefiting from the high utilization degree of active materials and rapid charge transport, the mass activity for oxygen reduction and evolution reactions of the ultrathin Co₃O₄/CC electrode is more than 10 times higher than that of the carbon cloth loaded with commercial Co₃O₄ nanoparticles. Compared to the commercial Co₃O₄/CC electrode, the flexible Zn–air battery using ultrathin Co₃O₄/CC electrode exhibits excellent rechargeable performance and high mechanical stability. Furthermore, the flexible Zn–air battery is integrated with a flexible display unit. The whole integrated device can operate without obvious performance degradation under serious deformation and even during the cutting process, which makes it highly promising for wearable and roll‐up optoelectronics.  相似文献   

    

Yanying Lu  Pengfei Zhou  Kaixiang Lei  Qing Zhao  Zhanliang Tao  Jun Chen 《Liver Transplantation》2017,7(7)

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Sophie S.W. Wang  Ching‐Min Huang  Yi‐Wei Lee  Michael Isaac Chen  Szu‐An Chuang  Shu‐Hua Chen  Ying‐Wei Lu  Chun‐Cheng Lin  Ka‐Wo Lee  Wen‐Hung Hsu  Kun‐Pin Wu  Yu‐Ju Chen 《Proteomics》2017,17(11)

We reported an integrated platform to explore serum protein variant pattern in cancer and its utility as a new class of biomarker panel for diagnosis. On the model study of serum amyloid A (SAA), we employed nanoprobe‐based affinity mass spectrometry for enrichment, identification and quantitation of SAA variants from serum of 105 gastric cancer patients in comparison with 54 gastritis patients, 54 controls, and 120 patients from other cancer. The result revealed surprisingly heterogeneous and most comprehensive SAA bar code to date, which comprises 24 SAA variants including SAA1‐ and SAA2‐encoded products, polymorphic isoforms, N‐terminal–truncated forms, and three novel SAA oxidized isotypes, in which the variant‐specific peptide sequence were also confirmed by LC‐MS/MS. A diagnostic model was developed for dimension reduction and computational classification of the 24 SAA‐variant bar code, providing good discrimination (AUC = 0.85 ± 3.2E?3) for differentiating gastric cancer group from gastritis and normal groups (sensitivity, 0.76; specificity, 0.81) and was validated with external validation cohort (sensitivity, 0.71; specificity, 0.74). Our platform not only shed light on the occurrence and modification extent of under‐represented serum protein variants in cancer, but also suggested a new concept of diagnostic platform by serum protein variant profile.  相似文献   

    

Qingzhao Feng  Xionglin Wu  Fuchao Li  Beibei Ning  Xiaofeng Lu  Yin Zhang  Ying Pan  Wenxian Guan 《蛋白质与细胞》2017,8(2):114-122

Increasing attention is focused on the down-regulation of miRNAs in cancer process. Nuclear receptor subfamily 2 (NR2F2, also known as COUP-TFⅡ) is involved in the development of many types of cancers, but its role in gastric cancer remains elusive. In this experiment, oncomine and Kaplan-meier database revealed that NR2F2 was up-regulated in gastric cancer and that the high NR2F2 expression contributed to poor survival. MicroRNA-27b was targeted and down-regulated by NR2F2 in human gastric cancer tissues and cells. The ectopic expression of miR-27b inhibited gastric cancer cell proliferation and tumor growth in vitro and in vivo. Assays suggested that the overexpression of miR-27b could promote MGC-803 cells' migration and invasion and retard their metastasis to the liver. In addition, down-regulation of miR-27b enhanced GES-1 cells' proliferation and metastasis in vitro. These findings reveal that miR-27b is a tumor suppressor in gastric cancer and a biomarker for improving patients' survival.  相似文献   

    

Jianrong Feng  Shijuan Fu  Xuan Cao  Hao Wu  Jing Lu  Ming Zeng  Lin Liu  Xue Yang  Yuequan Shen 《蛋白质与细胞》2017,8(7):538-543

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Pan-Pan Lu  Min-Hao Chen  Guang-Chun Dai  Ying-Juan Li  Liu Shi  Yun-Feng Rui 《World journal of stem cells》2020,12(11):1255-1275

There is accumulating evidence of an increased incidence of tendon disorders in people with diabetes mellitus. Diabetic tendinopathy is an important cause of chronic pain, restricted activity, and even tendon rupture in individuals. Tenocytes and tendon stem/progenitor cells (TSPCs) are the dominant cellular components associated with tendon homeostasis, maintenance, remodeling, and repair. Some previous studies have shown alterations in tenocytes and TSPCs in high glucose or diabetic conditions that might cause structural and functional variations in diabetic tendons and even accelerate the development and progression of diabetic tendinopathy. In this review, the biomechanical properties and histopathological changes in diabetic tendons are described. Then, the cellular and molecular alterations in both tenocytes and TSPCs are summarized, and the underlying mechanisms involved are also analyzed. A better understanding of the underlying cellular and molecular pathogenesis of diabetic tendinopathy would provide new insight for the exploration and development of effective therapeutics.  相似文献   

    

Sha Yu  Wen‐xia Chen  Wei Lu  Chao Chen  Yihua Ni  Bo Duan  Bin Wang  Huijun Wang  Zheng‐min Xu 《Molecular Genetics & Genomic Medicine》2020,8(5)

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Wei He  Jibin Lu 《Molecular Genetics & Genomic Medicine》2020,8(2)

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Jidong Liu  Guolian Ding  Kexin Zou  Ziru Jiang  Junyu Zhang  Yunhua Lu  Antonella Pignata  Eric Venner  Pengfei Liu  Zhandong Liu  Michael F. Wangler  Zheng Sun 《Molecular Genetics & Genomic Medicine》2020,8(3)

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