大鼠肝及脑组织中微粒体血红素加氧酶同工酶研究

采用离子交换层析和免疫印迹法分离、纯化和分析血红素和苯肼诱导后大鼠肝脏、脑组织.结果显示：纯化诱导后的大鼠肝脏,获得 HO-1和 HO-2,前者活性高于后者为2∶1.未诱导的大鼠肝脏仅获得HO-2,但诱导剂作用后,HO-1活性明显增加,而HO-2未见改变.HO-1和HO-2表观分子质量分别为30 ku和36 ku.诱导剂未作用的肝脏及作用的脑层析后仅获得HO-2活性的洗脱峰.免疫印迹法检测发现大鼠肝脏HO-2抗体与脑HO-2间有交叉反应,与肝脏HO-1无反应.实验表明在诱导剂作用的大鼠肝脏内含HO-1和HO-2同工酶,其中HO-1为诱导型酶.诱导剂作用的脑仅含HO-2.两种构型在表观分子质量,诱导性和免疫化学特性方面明显不同.     

DNA损伤与细胞周期调控

DNA损伤和损伤后修复可引起细胞周期阻滞,这一事件由三个阶段组成：损伤的识别,损伤信号的传递以及细胞周期阻滞.在某些情况,这种细胞周期阻滞会失效.     

控灌对小麦植株体内激素含量与籽粒灌浆速率的影响

利用防雨棚研究了控灌对小麦植株体内激素含量与籽粒不乐速率的影响。结果表明：随控水加重,小麦旗叶、根系和籽粒中ＡＢＡ含量迅速达到高峰,然后快速降低;控水愈重,ｉ－ＰＡｓ峰值和籽粒灌浆速率峰值出现愈早,尔后下降,从而加速了植株衰老进程,导致产量降低。籽粒灌浆速率变化与旗叶,根主籽粒中激素含量变化呈极显著相关,且上述性状在相对含水量６０％处理下与８０％（对照）相比无显著差异,从而为小麦节水栽培提供了依据     

Tissue Variance of Salicylic Acid-sensitive Catalase and Enhancement of Disease Resistance with Exogenous Salicylic Acid in Maize

The response to pathogen mediated by salicylic acid (SA) in plant required not only a high level of SA but also an effective SA signal perception and transduction mechanism. The maize ( Zea mays L. ) leaves contained extremely low level of free SA. Catalases from different maize tissues also exhibited different sensitivity to SA. Catalases from leaves were sensitive to SA, but roots contained SA-insensitive eatalases, indicating the presence of different tissue specific catalase isozymes. It seems that there is such an effective mechanism signal sensitization and signal conductance in maize leaves just the same as in tabacco and Arabidopsis. The ability of resistance to Helminthosporium turcicum Pass. in maize leaves can be enhanced by SA.     

Effects of Fatty Acids on Lipid Composition and Function of Tonoplast Vesicles in Barley Seedlings Under Salt Stress

When barley ( Hordeum vulgare L. ) seedlings were treated with 100 mmol/L NaC1 for 2 d, the index of unsaturated fatty acid (IUFA) increased in the tonoplast vesicles that were isolated from the seedlings mots of two barley cultivars with different salt tolerance, whereas no change were observed when the seedlings were treated with exogenous fatty acids with different satumbility. Exogenous stearic acid and linoleic acid decreased Na + absorption and transportation to the shoots, increased K + absorption and transportation, decreased the leakage of electrolytes, and increased the phospholipid and galactose contents of lipids in tonoplast, enhanced the activities of tonoplast H+ -ATPase and H+ -PPase. This is consistent with the results that the two fatty acids, linoleic acid and stearic acid, regulate ion absorption and distribution, mitigate salt stress to some extent, the effects of linoleic acid being more the latter.     

人降钙素类似物的合成及活性研究

通过对鲑鱼降钙素和人降钙素结构的比较,设计并合成了人降钙素类似物ｍｈＣＴ－２。利用空气氧化获得分子内二硫键,经分离纯化,产物达ＨＰＬＣ及毛细管电泳均一,蛋白质序列分析和质谱分析符合理论值。在大鼠降血钙生物活性测定中,利用量反应平行线法进行ｍｈＣＴ－２的生物效价判定达２０００ＩＵ／ｍｇ,比ｈＣＴ的效价高一个数量级。     

用三色荧光原位杂交(Three-Color FISH)检测小鼠精子中染色体数目异常的研究

用小鼠X、Y和8号染色体特异的DNA探针,与经DTT（dithiotreitol）和LIS（lithium-3,5-diiodosalicylicacid）解聚的小鼠附单精子进行三色荧光原位杂交（fluoresceoceinsituhybridization,FISH）,以检测精子中的染色体数目异常,并与MMⅡ染色体分析比较．结果表明精子三色FISH具有以下优点和特点：（1）方法敏感稳定,且简便快速;（2）在每一个体至少分析10000尾精子的基础上计算非整倍体单,因此结果更为准确;（3）能检测多倍体即减数分裂停止的发生率及停止的时期;（4）不仅能测定发生于试数分裂Ⅰ（MI）的染色体分离异常,还能检测发生于减数分裂Ⅱ（MII）的不分离和丢失．并对探针的选用、分析标准的建立以及三色FISH用于精于染色体分析的必要性等进行了讨论．     

细叶益母草叶表面腺毛多样性及发育形态学研究

本文研究了分布在细叶益母草(Leonurus sibiricus)叶表面三种腺毛的发育过程,在此基础上,对2-细胞头状腺毛、4-细胞头状腺毛和8-细胞盾状腺毛的多样性特征进行了讨论。     

半野生糜鹿集群行为的初步研究

糜鹿在半野生状态下喜集群括动,不同月份集群的大小以及各种组群出现的频率均有所变化。半野生糜鹿有5种组群类型：雄鹿群、母仔群、混合群、雄仔群和仔鹿群。其中混合群出现频率最高,集群最大,是其主要的组群类型,雄鹿群最为稳定,母仔群中采食组群所占比例和组群大小都大于非采食括动的组群,雄仔群和仔鹿群出现频次较少。麋鹿发情期与非发情的集群有明显差异,不同生境内的集群也有所不同。