外源钙调素对粟酒裂殖酵母细胞增殖的影响

外源钙调素（CaM）对粟酒裂殖酵母（Schizosaccharomycespombe）细胞增殖的影响。实验结果表明外源CaM能明显抑制粟酒裂殖酵母细胞的增殖,其作用方式是延长了粟酒裂殖酵母细胞生长的延滞期。抗粟酒裂殖酵母CaM抗体、TFP及Phenyl-SepharoseCL－4B能降低CaM对细胞生长的抑制作用,而Ca2+及Ca2+螫合剂EGTA对CaM的抑制作用均无影响。以上结果提示,外源CaM对粟酒裂殖酵母细胞增殖的抑制作用可能是由于胞外CaM激活了细胞膜上的Ca2+泵,使胞内Ca2+浓度降低所致。     

多分DNA病毒及其在寄生蜂与寄主关系中的作用

分DNA病毒(PDV)在协调寄生蜂与寄主的相互关系中起重要作用。该文就PDV 的特征、PDV与寄生蜂的相互关系、PDV对鳞翅目寄主的免疫抑制及发育调节等方面进行综述。     

南京汉族群体肺癌易感性相关基因的研究

为了探讨南京汉族群体肺癌易感性相关基因,我们采用1:1病例对照研究方法,以PCR—RFLP技术检测了152对肺癌和健康对照的CYP1A1、CYP2E1、GSTM1、GSTT1、GSTP1、mEH和NQO1基因的基因型并分析其与肺癌的相关性。结果发现携带CYP1A1突变基因型（wt/mt和mt/mt）的个体明显增加患肺鳞癌的风险(OR=2.31,95%CI=1.23-4.36);GSTT1（-）基因型可使肺癌发生的风险增加2.06倍（95%CI=1.30-3.24）;具有NQO1wt/mt与mt/mt基因型者发生肺癌的风险也有所增高（OR=1.66,95CI%=1.01-2.74）; CYP1A1突变基因型与GSTT1缺失基因型、CYP1A1突变基因型与NQO1突变基因型对肺癌的发生存在协同作用,同时具有两种易感基因型的个体更容易发生肺癌。研究结果表明,CYP1A1、GSTT1、NQO1基因可能与南京汉族群体肺癌遗传易感性有关,基因型之间的联合检测更有助于高危人群的筛选。Abstract: To investigate the genes related to lung cancer susceptibility in Nanjing Han population, China, a 1:1 matched case-control study was performed in which 152 hospital controls were matched to the 152 original lung cancer cases. The polymorphisms of CYP1A1, CYP2E1, GSTM1, GSTT1, GSTP1, mEH and NQO1 genes were analyzed by PCR—RFLP assay. The results showed that the heterozygote and mutation homozygote genotypes of CYP1A1 were related to the risk of squamous cell carcinoma (OR=2.31, 95%CI=1.23-4.36). The risk of suffering from lung cancer was increased 2.06-fold in the individuals with GSTT1（-） genotype (95%CI= 1.30-3.24). The genotype of NQO1 wt/mt and mt/mt was found also to be associated with the risk of lung cancer (OR=1.66,95%CI=1.01-2.74). It was shown that there was no difference in the genotype distribution of CYP2E1, GSTM1, GSTP1 or mEH between cases and controls. Furthermore, stratified analysis suggested that the combination of genotypes of both CYP1A1 and GSTT1 enzymes had a synergistic action in risk of lung cancer (OR=3.41, 95%CI =1.77-6.55). Similarly, there was a cooperation between CYP1A1 mutation genotype and NQO1 mutation genotype (OR=2.45, 95%CI=1.13-5.31). This study suggested that CYP1A1, GSTT1 and gene NQO1 polymorphisms might be associated with the susceptibility to lung cancer in Nanjing Han population. Analysis of gene-gene interactions was helpful to identification of susceptible individuals and screening high-risk population to lung cancer.     

History and progress of the genomics studies in the model system of perennial plant species

Strong justifications have been developed for why woody plants should be viewed as mod-el systems in plant biology. The genus Populus possesses many characteristics that are conductive to functional genomic studies,and therefore leads to its emergence as a model system in extrapolating findings in perennial plant species that are different from annual herbaceous plants. With the pro-ceeding of the whole genome sequencing,poplars will be act as a wide reference for functional ge-nomics studies in perennial plant species and will also contribute towards answering some fundamen-tal scientific questions. This paper reviewed the history and progress of the poplar genome studies and the potential keen topics in the future. The contents mainly address on: （1）the somatic genet-ics studies in Populus;（2）the genomics studies carried out in Populus,including genetic map-ping,genome sequ encing,physical map construction,microarray analysis and linkage disequilibri-um analysis;（3）the potential application of the genome information of Populus for facilitating our understanding of some basic scientific questions.     

华南虎圈养种群的统计分析

对《华南虎谱系》307 只登录个体的统计表明, 其圈养的历史有46 年。在人工繁殖的38 年中产122 胎287仔。现存圈养华南虎分散在全国22 家单位, 有57 只个体, 性比M∶F = 1185 , 能成功繁殖后代的个体仅5 只(只占818 %) , 是一个衰退的种群。通过SPARKS 和GENES 软件分析发现: 从1977 年开始, 圈养种群的基因多样性便逐渐下降而近交系数却不断增加。     

秋茄对镉-甲胺磷混合物的吸收积累及致毒作用

本文采用砂培实验,测定了红树植物秋茄幼苗对镉和镉-甲胺磷的吸收积累和净化作用,探讨了镉-甲胺磷混合物对秋茄的毒性作用。实验结果表明:虽然100mg·L^-1甲胺磷并不影响秋茄对镉的净化作用,但100mg·L^-1甲胺磷的存在却加强了镉对秋茄的毒性作用。这可能是由于镉对秋茄幼苗的膜脂过氧化作用,改变秋茄幼苗细胞膜的通透性和流动性,使甲胺磷易于从水体向秋茄幼苗的转移,从而加强了镉对秋茄的毒性效应。因此,在确定红树植物对镉“耐受临界浓度”时,建议考虑其它化合物与镉对红树植物的联合毒性作用。     

广州街口至良口高速公路对水环境影响的评价分析

在对区域水环境现状进行调查分析的基础上,预测了街口至良口高速公路建设工程施工期与运营期对水环境的影响,特别是对流溪河水质的影响程度进行了详细分析。评价和预测结果表明:流溪河水质现状良好,符合相应的水环境功能要求;该工程中两大桥的水下施工分别对下游100和150m范围内水质影响较大,影响属局部短暂性;运营期影响程度较小,通过采取环保措施,对水环境产生的不利影响可以得到减轻或消除。     

Developmental Changes in Relation to Desiccation Tolerance of Archontophoenix alexandrae (Palmae) Seeds

Germination of Archontophoenix alexandrae seeds and embryos were studied under gradient water content treatments throughout the seed development phases of maturation in 2005 to investigate seed desiccation tolerance and storage characteristics. During the maturation process, seed water content decreased gradually from55 DAF (days after flowering) to 70 DAF, and seeds reached the maximum dry-weight at 90 DAF. Seed germinability appeared after 60 DAF. Seeds germinated with a temperature range from15℃- 40℃ under alternating photoperiod (14 h light, 10 h dark, 12μmol m^{- 2}s ^{- 1} ), while the best germination percentage was obtained between 30℃- 35℃. A maximum germination capacity reached at 70 DAF. However, seed germination was greatly inhibited by light. Desiccation tolerance of seeds and embryos increasedgradually from 55 DAF to 90 DAF and reached the maximum at 90 DAF with a semilethal water content of 0.18 g/g ( seed) and 0.3 g/g ( embryo) respectively. Rapid dehydration maintained higher seed germination percentage than thatof slow dehydration when drying to the same water content. Seeds with without water content treatments failed to germinate after 1 month storage under - 18℃, whereas appropriate desiccation treatment prolonged seed longevity under 4℃, 10℃ and 15℃ storage temperatures. It revealed obviously the recalcitrant characteristics of Archontophoenix alexandrae seeds torage behaviour which are tolerant toward neither deep desiccation nor low temperatures.     

二倍体和四倍体泥鳅染色体组型比较

根据陈景星1 的论述,分布于中国境内的泥鳅属(Misgurnus) 的鱼类共有三种:北方泥鳅 (M.bipartitus) 、黑龙江泥鳅(M.mohoity) 和泥鳅(M.anguillicaudatus) 。       

克瓦厚厉螨雄螨及后若螨描述(蜱螨亚纲:革螨股:厚厉螨科)

克瓦厚厉螨（Pachylaelaps kievati Davydova,1971）,在所查到的献中仅描述了雌螨（KoponeBa,1977）,本描述其雄螨及后若螨。