云南古蚖属四新种(原尾纲：古蚖目)

本文报道了在云南新发现的 4种古蚖:热海古蚖Eosentomonrehaiense、腾冲古蚖E tengchongense、大孔古蚖E megastigma和古永古蚖E guyongense。它们的腹板Ⅳ～Ⅶ节毛序都是 6 /9型的。     

老虎感染猫传染性鼻—结膜炎病毒的研究

用猫肾传代细胞从发病虎的病料中分到了一株杯状病毒粒子样病毒。该病毒大小为３５～３９ｎｍ、无囊膜、在胞浆中病毒前体呈晶格状排列,抗乙醚、不能被５－ＩＵＤＲ所抑制,能被来源于标准疫苗的猫传染性鼻－结膜炎病毒（或杯状病毒Ｆｅｌｉｎｅ ｃａｌｉｃｉｖｉｒｕｓ,ＦＣＶ）抗血清所中和,人工感染猫出现典型的ＦＣＶ感染症状。ＲＴ－ＰＣＲ能扩增出与设计值相符的电泳带,ＰＣＲ产物直接测序结果与Ｇｅｎｅｂａｎｋ发表的Ｆ     

构建基因工程菌发酵产生维生素C的前体2-酮基-L-古龙酸

从棒状杆菌ＳＣＢ３０５８克隆得到两个２,５－ＤＫＧ＾＊＊还原酶基因后,构建了两个能够表达２,５－ＤＫＧ还原酶的基因工程大肠杆菌ＢＬ２１（ＤＥ３）ＰＥＴ９ａⅡ和ＤＨ５α（ｐＢＬ４）和一个基因工程欧文氏菌ＥＲ９７。２,５－ＤＫＧ还原酶基因分别受控于ＰＬ或Ｔ７启动子,通过加入ＩＰＴＧ或提高温度进行诱导,ＳＤＳ－ＰＡＧＥ和酶活测定确定它们在诱导后得到了高表达,用细胞抽提液在加入辅酶ＮＡＤＰＨ的体外实验中转     

中国巨蟹蛛属（蜘蛛目：巨蟹蛛科）一新种

本文描述了产于云南省的巨蟹蛛科一新种：双球巨蟹珠,Ｈｅｔｅｒｏｐｏｄａ ｂｉｂｕｌｂａ ｓｐ．ｎｏｖ．。本文量度单位：ｍｍ。模式标本保存在湖南师范大学生命科学学院。     

K_(ATP)通道开放剂对颈动脉窦压力感受器反射的易化作用

在 30只隔离灌流颈动脉窦区的麻醉大鼠 ,观察了KATP通道开放剂 (cromakalim ,Cro)对颈动脉窦压力感受器反射的影响。结果如下 :( 1)以Cro ( 10 μmol/L)隔离灌流大鼠左侧颈动脉窦区时 ,压力感受器机能曲线向左下方移位 ,曲线最大斜率 (PS)由 0 36± 0 0 1增至 0 48± 0 0 1kPa/kPa (P <0 0 0 1) ,反射性血压下降幅度 (RD)由 5 78± 0 14增至 7 87± 0 12kPa (P <0 0 0 1) ;阈压 (TP)、平衡压 (EP)和饱和压 (SP)则分别从 8 34± 0 35 ,12 71± 0 2 5和 2 4 89±0 2 5下降至 6 41± 0 0 9kPa,11 78± 0 2 4kPa ,2 2 5 6± 0 16kPa (P <0 0 1～ 0 0 0 1)。其中RD ,PS和TP的变化呈明显的剂量依赖性。 ( 2 )用KATP通道阻断剂格列苯脲 (glibenclamide,10 μmol/L)预处理后 ,Cro的上述反射效应即被阻断。( 3)先给予腺苷 (adenosine,12 5 μmol/L)则可以加强Cro对压力感受器反射的影响。以上结果表明 ,KATP通道开放剂Cro对大鼠颈动脉窦压力感受器反射有易化作用 ,此作用是由KATP通道开放剂引起窦壁扩张而牵张压力感受器所致     

外源钙调素对粟酒裂殖酵母细胞增殖的影响

外源钙调素（CaM）对粟酒裂殖酵母（Schizosaccharomycespombe）细胞增殖的影响。实验结果表明外源CaM能明显抑制粟酒裂殖酵母细胞的增殖,其作用方式是延长了粟酒裂殖酵母细胞生长的延滞期。抗粟酒裂殖酵母CaM抗体、TFP及Phenyl-SepharoseCL－4B能降低CaM对细胞生长的抑制作用,而Ca2+及Ca2+螫合剂EGTA对CaM的抑制作用均无影响。以上结果提示,外源CaM对粟酒裂殖酵母细胞增殖的抑制作用可能是由于胞外CaM激活了细胞膜上的Ca2+泵,使胞内Ca2+浓度降低所致。     

多分DNA病毒及其在寄生蜂与寄主关系中的作用

分DNA病毒(PDV)在协调寄生蜂与寄主的相互关系中起重要作用。该文就PDV 的特征、PDV与寄生蜂的相互关系、PDV对鳞翅目寄主的免疫抑制及发育调节等方面进行综述。     

南京汉族群体肺癌易感性相关基因的研究

为了探讨南京汉族群体肺癌易感性相关基因,我们采用1:1病例对照研究方法,以PCR—RFLP技术检测了152对肺癌和健康对照的CYP1A1、CYP2E1、GSTM1、GSTT1、GSTP1、mEH和NQO1基因的基因型并分析其与肺癌的相关性。结果发现携带CYP1A1突变基因型（wt/mt和mt/mt）的个体明显增加患肺鳞癌的风险(OR=2.31,95%CI=1.23-4.36);GSTT1（-）基因型可使肺癌发生的风险增加2.06倍（95%CI=1.30-3.24）;具有NQO1wt/mt与mt/mt基因型者发生肺癌的风险也有所增高（OR=1.66,95CI%=1.01-2.74）; CYP1A1突变基因型与GSTT1缺失基因型、CYP1A1突变基因型与NQO1突变基因型对肺癌的发生存在协同作用,同时具有两种易感基因型的个体更容易发生肺癌。研究结果表明,CYP1A1、GSTT1、NQO1基因可能与南京汉族群体肺癌遗传易感性有关,基因型之间的联合检测更有助于高危人群的筛选。Abstract: To investigate the genes related to lung cancer susceptibility in Nanjing Han population, China, a 1:1 matched case-control study was performed in which 152 hospital controls were matched to the 152 original lung cancer cases. The polymorphisms of CYP1A1, CYP2E1, GSTM1, GSTT1, GSTP1, mEH and NQO1 genes were analyzed by PCR—RFLP assay. The results showed that the heterozygote and mutation homozygote genotypes of CYP1A1 were related to the risk of squamous cell carcinoma (OR=2.31, 95%CI=1.23-4.36). The risk of suffering from lung cancer was increased 2.06-fold in the individuals with GSTT1（-） genotype (95%CI= 1.30-3.24). The genotype of NQO1 wt/mt and mt/mt was found also to be associated with the risk of lung cancer (OR=1.66,95%CI=1.01-2.74). It was shown that there was no difference in the genotype distribution of CYP2E1, GSTM1, GSTP1 or mEH between cases and controls. Furthermore, stratified analysis suggested that the combination of genotypes of both CYP1A1 and GSTT1 enzymes had a synergistic action in risk of lung cancer (OR=3.41, 95%CI =1.77-6.55). Similarly, there was a cooperation between CYP1A1 mutation genotype and NQO1 mutation genotype (OR=2.45, 95%CI=1.13-5.31). This study suggested that CYP1A1, GSTT1 and gene NQO1 polymorphisms might be associated with the susceptibility to lung cancer in Nanjing Han population. Analysis of gene-gene interactions was helpful to identification of susceptible individuals and screening high-risk population to lung cancer.     

History and progress of the genomics studies in the model system of perennial plant species

Strong justifications have been developed for why woody plants should be viewed as mod-el systems in plant biology. The genus Populus possesses many characteristics that are conductive to functional genomic studies,and therefore leads to its emergence as a model system in extrapolating findings in perennial plant species that are different from annual herbaceous plants. With the pro-ceeding of the whole genome sequencing,poplars will be act as a wide reference for functional ge-nomics studies in perennial plant species and will also contribute towards answering some fundamen-tal scientific questions. This paper reviewed the history and progress of the poplar genome studies and the potential keen topics in the future. The contents mainly address on: （1）the somatic genet-ics studies in Populus;（2）the genomics studies carried out in Populus,including genetic map-ping,genome sequ encing,physical map construction,microarray analysis and linkage disequilibri-um analysis;（3）the potential application of the genome information of Populus for facilitating our understanding of some basic scientific questions.     

华南虎圈养种群的统计分析

对《华南虎谱系》307 只登录个体的统计表明, 其圈养的历史有46 年。在人工繁殖的38 年中产122 胎287仔。现存圈养华南虎分散在全国22 家单位, 有57 只个体, 性比M∶F = 1185 , 能成功繁殖后代的个体仅5 只(只占818 %) , 是一个衰退的种群。通过SPARKS 和GENES 软件分析发现: 从1977 年开始, 圈养种群的基因多样性便逐渐下降而近交系数却不断增加。