高原鼢鼠和高原鼠兔骨骼无机化学成分的研究Ⅰ.常量元素

本文对高原鼢鼠和高原鼠兔骨骼中的无机常量元素K、N a、Ca、M g、P 和A l 进行了比较分析。研究结果表明, 高原鼢鼠骨骼中Ca、P、A l 的含量极显著地高于高原鼠兔(P <0.01) ,K 含量高原鼢鼠极显著地低于高原鼠兔(P < 0.001) , N a 和M g 的含量两者间无显著差异(P > 0.05) ; 骨骼各部位元素总量的分布顺序为: 高原鼢鼠下肢骨> 头骨> 脊柱; 高原鼠兔头骨> 下肢骨> 脊柱。15个元素对中, 大部分元素之间线性相关非常显著, 其中高原鼢鼠10对呈显著的线性相关, 高原鼠兔13对呈显著相关。     

重庆金佛山自然保护区种子植物区系新资料

报道重庆市南川金佛山自然保护区新记录植物10种,隶属8科9属,其中新分布属6个,新分布科1个。它们分别为樟科的木姜叶润楠Machilus litseifolia S.Lee、金缕梅科的小叶蚊母Distylium buxifolium(Hance)Merr.和小叶银缕梅Parrotia subaequalis(H.T.Chang)R.M.Hao et H.T.Wei、千屈菜科水苋菜Ammannia baccifera Linn.和多花水苋菜Ammannia multiflora Roxb.、桃金娘科的赤楠Syzygium buxifolium Hook.et Arn.、鹿蹄草科喜冬草Chimaphila japonica Miq.、狸藻科的高山捕虫堇Pinguicula alpina L.、菊科的异芒菊Blainvillea acmella(L.)Philipson和毛瓣杓兰Cypripedium fargesii Franch.。     

河南省发现宁波滑蜥

2011年7月、2012年7月和10月在河南伏牛山国家级自然保护区采集到5号宁波滑蜥(Scincella modesta)标本,系河南首次发现,标本保存于河南大学生命科学学院动物学标本室。     

新疆维、哈两民族粪样中奇异菌簇和产气柯林斯菌属水平与2型糖尿病的相关性研究

目的检测新疆维吾尔族、哈萨克族糖耐量正常人群及2型糖尿病患者粪便中奇异菌簇和产气柯林斯菌属的水平,分析奇异菌簇和产气柯林斯菌属与2型糖尿病的相关性。方法收集新疆维吾尔族、哈萨克族糖耐量正常人群及2型糖尿病患者血样和粪便样本,采用16S r DNA实时荧光定量PCR技术检测粪便样本中奇异菌簇和产气柯林斯菌属的水平;运用Pearson分析目标菌属水平与4组人群的空腹血糖(FBG)、年龄、身高、体重、体重指数(BMI)、腰围、胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDLC)、低密度脂蛋白胆固醇(LDL-C)的相关性。结果 (1)与哈萨克族糖耐量正常组比较,该民族T2DM组中奇异菌簇、产气柯林斯菌属水平均降低(P=0.012,P=0.024),两民族糖耐量正常组之间奇异菌簇、产气柯林斯菌属水平差异有统计学意义(P=0.025,P=0.007);(2)奇异菌簇水平与HDL-C(r=0.281,P=0.031)呈正相关,与体重(r=-0.514,P=0.000)、BMI(r=-0.400,P=0.002)、TC(r=-0.503,P=0.000)、LDL-C(r=-0.581,P=0.000)水平呈负相关;产气柯林斯菌属水平与HDL-C(r=0.550,P=0.000)水平呈正相关,与体重(r=-0.449,P=0.000)、BMI(r=-0.432,P=0.001)水平呈负相关。结论肠道中奇异菌簇和产气柯林斯菌属,可能与与新疆维吾尔族、哈萨克族2型糖尿病患者的脂代谢紊乱密切相关。     

The METTL5-TRMT112 N6-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation

Ribosomal RNAs (rRNAs) have long been known to carry chemical modifications, including 2′O-methylation, pseudouridylation, N⁶-methyladenosine (m⁶A), and N^6,6-dimethyladenosine. While the functions of many of these modifications are unclear, some are highly conserved and occur in regions of the ribosome critical for mRNA decoding. Both 28S rRNA and 18S rRNA carry single m⁶A sites, and while the methyltransferase ZCCHC4 has been identified as the enzyme responsible for the 28S rRNA m⁶A modification, the methyltransferase responsible for the 18S rRNA m⁶A modification has remained unclear. Here, we show that the METTL5-TRMT112 methyltransferase complex installs the m⁶A modification at position 1832 of human 18S rRNA. Our work supports findings that TRMT112 is required for METTL5 stability and reveals that human METTL5 mutations associated with microcephaly and intellectual disability disrupt this interaction. We show that loss of METTL5 in human cancer cell lines and in mice regulates gene expression at the translational level; additionally, Mettl5 knockout mice display reduced body size and evidence of metabolic defects. While recent work has focused heavily on m⁶A modifications in mRNA and their roles in mRNA processing and translation, we demonstrate here that deorphanizing putative methyltransferase enzymes can reveal previously unappreciated regulatory roles for m⁶A in noncoding RNAs.     

谷胱甘肽-硫-转移酶M2基因在小鼠生殖器官中的表达

利用半定量RT-PCR和原位杂交的方法检测Gstm2基因在成年雄性和雌性小鼠生殖器官中的表达,并初步评价其在生殖过程中的作用。在雄性小鼠的睾丸、附睾、输精管和雌性小鼠的卵巢、输卵管、子宫、胎盘中,半定量RT-PCR的方法均检测到Gstm2的表达,在胎盘中表达水平较低,其余组织表达水平较高。利用原位杂交的方法在睾丸的间质细胞检测出较强的信号,在附睾中有微弱的信号,而输精管上皮细胞没有检测到信号;在输卵管上皮细胞和妊娠第3d的子宫上皮细胞中检出较强的信号。由于Gstm2在RNA水平在小鼠的生殖器官中广泛表达,因此我们推测Gstm2可能在小鼠精子发生、睾酮合成、精子的成熟和运输、卵子的发生和运输、胚胎着床等生殖过程中发挥作用,此结果为深入研究Gstm2在生殖生理中的功能打下基础。     

多异瓢虫气味结合蛋白HvarOBP2的基因克隆及配体结合特性分析

【目的】本研究旨在克隆多异瓢虫Hippodamia variegata气味结合蛋白(odorant-binding proteins, OBPs)基因HvarOBP2,并解析这一蛋白的配体结合特征。【方法】采用BLAST和生物信息学技术从多异瓢虫成虫触角转录组中鉴定候选气味结合蛋白基因HvarOBP2,PCR特异扩增HvarOBP2基因全长cDNA序列;通过体外原核表达和亲和柱层析等手段获得HvarOBP2重组蛋白;运用荧光竞争结合实验测定重组蛋白HvarOBP2与40种植物挥发物组分以及24种蚜虫相关挥发物组分的结合能力;使用PyMOL1.9.0进行重组蛋白HvarOBP2与4种配体(β-紫罗兰酮、邻苯二甲酸二丁酯、油酸和橙花叔醇)的分子对接模拟。【结果】HvarOBP2(GenBank登录号：OK340816)开放阅读框(open reading frame, ORF)长447 bp,其编码蛋白N端有17个氨基酸组成的信号肽,具有6个保守的半胱氨酸位点,属于classical OBPs亚家族。荧光竞争结合实验结果表明,重组蛋白HvarOBP2与植物挥发物组分油酸(解离常数K     

A novel gene delivery system targeting cells expressing VEGF receptors

Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors.GV1,GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex.Using pSV2-β-galactosidase as a reporter gene,it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) and human malignant melanoma cell lines (A375) in vitro.In vivo experiments,exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO,human malignant melanoma A375 and human hepatoma graft in nude mice.This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice.These results are correlated with the relevant receptors(flt-1,flk-1/KDR) expression on the targeted cells and tissues.