Cloning, sequence analysis and expression of gene alyVI encoding alginate lyase from marine bacterium Vibrio sp. QY101.

The gene (alyVI) encoding an alginate lyase of marine bacterium Vibrio sp. QY101, which was isolated from a decaying thallus of Laminaria, was cloned using a strategy of combined degenerate PCR and long range-inverse PCR (LR-IPCR), then sequenced and expressed in Escherichia coli. Gene alyVI was composed of a 1014 bp open reading frame (ORF) encoding 338 amino acid residues. The calculated molecular mass of alyVI product is 38.4 kDa, but a signal peptide is cleaved off, leaving a mature protein of 34 kDa. AlyVI was purified from culture supernatants to electrophoretic homogeneity using affinity chromatography. AlyVI was most active at pH 7.5 and 40 degrees C in the presence of 1 mM ZnCl2. A nine-amino-acid consensus region (YXRESLREM), which was only found in polyguluronate lyases, was also observed in the amino-terminal region of AlyVI. However, AlyVI could degrade both M block and G block. These results indicate that a novel alginate lyase-encoding gene has been cloned.     

海甘蓝种子成熟过程中脂肪酸含量以及脱落酸和山梨醇的效应

海甘蓝种子在成熟过程中,棕榈酸、硬脂酸和亚麻酸的含量不断下降,而二十碳烯酸和芥酸的含量呈上升趋势。选用开花后２５～２７ｄ的海甘蓝幼胚分别在含不同浓度的ＡＢＡ或高渗透剂的培养基中培养１～３ｄ,发现其各种脂肪酸的变化趋势和种子自然成熟过程中脂肪酸的变化相似,说明ＡＢＡ或高渗透剂可能是种子成熟过程中各种脂肪酸合成和相互转化所需的条件。     

肿瘤坏死因子α对白血病细胞作用的^31P核磁共振研究

木文用３１Ｐ核磁共振（ＮＭＥ）观察了人淋巴瘤白血病细胞系Ｍｏｌｔ－４和人早幼粒白血病细胞系ＨＬ－６０的细胞代谢。Ｍｏｌｔ－４和ＨＬ－６０细胞系的３１Ｐ谱主要由磷酸单酯、无机磷（Ｐｉ）、双磷酸双酯、ＡＴＰ的共振峰组成。３１Ｐ谱显示Ｍｏｌｔ－４细胞在受肿瘤坏死因子α（ＴＮＦ－α）作用后,ＡＴＰ／Ｐｉ比值降低。ＨＬ－６０细胞在受ＴＮＦ－α作用后,ＡＴＰ减少。３１Ｐ－ＮＭＲ提供了一种非损伤性研究细胞代谢的重要方法。     

kar交配法转移YACs至新宿主的研究

利用改进的kar交配法,将一个含有340kb人基因组DNA的YAC片段的供体酵母菌株YAC23与受体菌株YLB504进行交配,以选择平板对所形成的候选YAC导入菌进行筛选。经PCR分析,候选YAC导入菌在404bp处有一个扩增带,即具有受体菌株的交配型(MAT α)。进一步用脉冲电泳进行核型鉴定,证实YACs己成功地进入受体,实现了YACs从一个宿主到另一个宿主之间的转移。     

下盾螨属一新种──（蜱螨亚纲：厉螨科）

本文记述下盾螨新种Ｈｙｏａｓｐｉｓｓｏｒｅｃｉｓｓｐ．ｎｏｖ,并讨论与近似种Ｈ．ＨｒｙｄｉＳａｍｓｉｎａｋ１９６１的鉴别特征。模式标本采自青海互助土族自治县的暗色ＳｏｒｅｘｓｉｎａｌｉｓＴｈｏｍａｓ,１９１２体上。文内量度单位均为μｍ,标本存放在青海省地方病防治研究所,西宁。     

Egg presence, egg loss, and female mate preferences in the sand goby (Pomatoschistus minutus)

We studied the effect of egg presence on female mate choicein a fish with paternal care. Females who were allowed a freechoice between two males mated within a shorter time than femaleswho were randomly assigned to a particular male. When a secondfemale was allowed to choose among the males, she preferredthe same male as the previous female. This result shows thatfemales are concordant in their mate choice. When the initialfemale was randomly assigned to mate with one of two males (forcedchoice), the second female mated randomly with respect to thefirst one. Thus females do not prefer males with eggs. If theinitial female was given a free choice, but the eggs were removedfrom the chosen male, the test female mated randomly. When boththe males initially had mated but one randomly determined male'seggs were removed, the test female preferred the male who wasstill guarding eggs. These experiments show that females avoidspawning in unsuccessful nests. When the females in the freechoice/egg removal experiment mated with the unsuccessful malethere was a considerably bigger size difference in favor ofthis male than when the females mated with the other male. Weconclude that female sand gobies show clear mate preferences,but that they do not prefer males with eggs over males withouteggs. They do, however, avoid mating with males guarding unsuccessfulnests. We therefore suggest that egg loss could be an importantfactor selecting for egg preference.     

龙血树真菌群及其对血竭形成的影响

从柬埔寨龙血树（Ｄｒａｃａｅｎａｃｏｃｈｉｎｃｈｉｎｅｎｓｉｓ）茎杆中分离到３０３株真菌,其中镰刀菌属（Ｆｕｓａｒｉｕｍ）菌株占总分离频率的５２％,其次是短梗霉（Ａｕｒｅｏｂａｓｉｄｉｕｍ）和枝孢霉（Ｃｌａｄｏｓｐｏｒｉｕｍ）。通过活体接种对血竭产生的影响试验表明,对血竭形成起重要作用的真菌主要是禾谷镰刀菌龙血树变种（Ｆ．ｇｒａｍｉｎｕｍｖａｒ．ｄｒａｃａｅｎａ）等４株红色镰刀菌,可使血竭形成量提高６６％－１２０％。     

四川大鲵寄生吸虫一新属新种（吸虫纲：复殖目）

本文描述寄生在大鲵肠道内隐殖科,隐殖亚科吸虫一新种,沐川新鲵居吸虫Ｎｅｏａｎｄｒｉｎｃｏｌａ ｍｕｃｈｕａｎｅｎｓｉｓ ｓｐ．ｎｏｖ．并建立了新属,即新鲵居吸虫属Ｎｅｏａｎｄｒｉｎｃｏｌａ ｇｅｎ．ｎｏｖ．标本采自四川省沭县。模式标本保存在四川农业大学兽医学系。     

中国孔蛛属一新种（蜘蛛目：跳蛛科）

本文记述蜘蛛目跳蛛科孔蛛属一新种,宋氏孔蛛,新种Ｐｏｒｔｉａ ｓｏｎｇｉ ｓｐ．ｎｏｖ．,标本采自甘肃文县。