EPR studies with 77Se-enriched (NiFeSe) hydrogenase of Desulfovibrio baculatus. Evidence for a selenium ligand to the active site nickel

The periplasmic hydrogenase containing equivalent amounts of nickel and selenium plus non-heme iron [NiFeSe) hydrogenase) has been purified from cells of the sulfate reducing bacterium Desulfovibrio baculatus (DSM 1748) grown on a lactate/sulfate medium containing natural Se isotopes and the nuclear isotope, 77Se. Both the 77Se-enriched and unenriched hydrogenases were shown to be free of other hydrogenases and characterized with regard to their Se contents. EPR studies of the reduced nickel signal generated by redox titrations of the enriched and unenriched (NiFeSe) hydrogenases demonstrated that the gx = 2.23 and gy = 2.17 resonances are appreciably broadened by the spin of the 77Se nucleus (I = 1/2). This observation demonstrates unambiguously that the unpaired electron is shared by the Ni and Se atoms and that Se serves as a ligand to the nickel redox center of the (NiFeSe) hydrogenase.     

Application of a panel of antiganglioside monoclonal antibodies to cytologic specimens.

The cytologic evaluation of poorly differentiated tumors frequently poses a diagnostic dilemma as to the tissue of origin. To assess the diagnostic utility of monoclonal antibodies (MAbs) in these situations, we applied a panel of three highly purified MAbs specific for tumor-associated ganglioside epitopes to a diverse series of cytologic specimens. The panel was composed of DMAb-3, reactive with the epitope GalNAc beta 1-4 (NeuAc alpha 2-3)Gal- of GM2; DMAb-7, reactive with the epitope (NeuAc alpha 2-8NeuAc alpha 2-3)Gal beta 1-4(Glc or GlcNAc)- of GD3 and 3'8'-LD1; and DMAb-20, reactive with the epitope GalNAc beta 1-4(NeuAc alpha 2-8NeuAc alpha 2-3)Gal- of GD2. The cytologic material consisted of air-dried Cytospin preparations prepared predominantly from fine needle aspirates and stained with the ABC immunohistochemical method. Positive reactivity was recognized when greater than 5% of tumor cells stained with the antibody; lesser reactivity was called negative. DMAb-3 stained 9/14 (64%) glial tumors, 4/13 (31%) nonglial central nervous system tumors, 1/21 (5%) melanomas, 7/38 (18%) non-small cell carcinomas (NSCC), 1/15 (7%) small cell carcinomas (SCC), 0/9 (0%) lymphomas/leukemias, 2/10 (20%) sarcomas, 1/7 (14%) miscellaneous tumors and 2/2 (100%) reactive fluids. DMAb-7 recognized 14/14 (100%) glial tumors, 9/13 (69%) non-glial central nervous system tumors, 19/22 (86%) melanomas, 19/43 (44%) NSCC, 5/15 (33%) SCC, 2/9 (22%) lymphomas/leukemias, 6/10 (60%) sarcomas, 1/7 (14%) miscellaneous tumors and 4/4 (100%) reactive fluids. DMAb-20 stained 6/14 (43%) glial tumors, 2/13 (15%) nonglial central nervous system tumors, 1/21 (5%) melanomas, 4/38 (10%) NSCC, 0/15 (0%) SCC, 0/9 (0%) lymphomas/leukemias, 1/10 (10%) sarcomas, 1/7 (14%) miscellaneous tumors and 1/3 (33%) reactive fluids. The GD3-reactive DMAb-7 recognized a large portion of many tumor types and thus is not diagnostically useful alone. DMAb-3 and DMAb-20 were more selective and showed the strongest reactivity for glial tumors and minimal reactivity for melanomas, small cell carcinomas, and lymphomas or leukemias. DMAb-3 and DMAb-20 may be useful as components of a larger panel of MAbs in distinguishing between poorly differentiated tumors in samples derived from the central nervous system.     

黑龙江绥滨地区晚侏罗世东荣组的沟鞭藻类

本文对黑龙江绥滨86-11孔的沟鞭藻类进行了初步研究,共描述化石10属16种(包括2新种,6未定种);其中不少是在我国首次描述的。这些化石分成两个组合,其优势分子分别为Gonyaulacysta jurassica和Amphorula delicata。根据组合内各分子的时代分布,两化石组合的时代分别为Late Oxfordian—Early Kimmeridgian和Portlandian期,并对其产出层位东荣组的时代作了讨论,认为属于晚侏罗世。     

蝌蚪（Bufo bufo andrewsi）血红细胞微核和核异常监测水质污染的研究

滇池是云南高原最大的淡水湖,是昆明地区工农业生产及人民生活用水的水源,又是昆明市的重要风景区。近年来,滇池水质污染,越来越引起人们的关注。昆明市的工业废水和生活污水经大观河等流入滇池,其中有毒有害物质多达30多种,包括镉、汞、砷、铅、铬、氟、有机磷、氰化物等。其中砷(Léonard et al., 1980;Katsuhiko,     

四川青城山优食蚜蝇属二新种记述（双翅目：食蚜蝇科）

本文记述四川青城山优食蚜蝇属Eupeodes 2新种:青优食蚜蝇Eupeodes (Eupeodes) qingchengshanensis和小优食蚜蝇Eupeodes(Eupeodes)parvus,均属于Eupeodes s.str.,亚属。模式标本保存于上海农学院昆虫标本室。     

利用泽兰实蝇控制紫茎泽兰的生防策略研究

本文根据野外泽兰实蝇种群15个世代的调查资料,利用契贝谢夫正交多项式拟合了泽兰实蝇、紫茎泽兰的空间格局。阐明了泽兰实蝇空间格局的序列变化及紫茎泽兰空间格局的特点;揭示了泽兰实蝇空间格局的特点受当地主风及寄主紫茎泽兰空间格局特点的影响;并从最优控制系统的角度对泽兰实蝇-紫茎泽兰系统作了初步探讨。首次提出了最佳释放虫量指标为每条虫占有10条枝条。这些结果为多点释放及定点多次释放泽兰实蝇防治紫茎泽兰这一生防策略提供了理论基础。     

四节金小蜂科在我国的首次发现并记述一新种(膜翅目:小蜂总科)

四节金小蜂科Tetracampidae是小蜂总科中的1个小科。1968年统计,全世界只有2亚科6属18种(Boucek and Askew,1968)。1971年,苏联的Sugonjaev根据在蒙古和苏联的哈萨克加盟共和国采到的标本描述了3新属1新亚科。Boucek于1988年又记述了澳大利亚的1个新属。这样,四节金小蜂科现在共包括3亚科10属31种(不含化石种)。我国迄今还未见有本科昆虫分布的报道。     

内蒙古草蛉研究Ⅱ.五新种及二新记录种(脉翅目:草蛉科)

内蒙古地处我国北部边疆,全境以高原为主,西部为戈壁沙漠,东部为宽阔草原,绝大多数地区属于温带大陆性气候;动物地理区划属于古北区中亚细亚区的内蒙河西干草原区。该区现已知草蛉3属16种(包括本文所记述的5新种和2新记录种),其分布特点是以狭义的草蛉属Chrysopa为主,而且以广布于华北地区的叶色草蛉Chrysopaphyllochroma Wesmeal为代表种,新种的模式标本分别保存在中国科学院动物研究所及北京农业大学昆虫标本室。