贲门失弛缓症可回收支架治疗的疗效评价

目的：观察评价可回收式支架治疗贲门失弛缓症的疗效。方法：选取来我院治疗的2003年11月～2012年7月收治的48例贲门失弛缓症患者,在DSA透视下行经口可回收食道支架置入,15-90天后将支架取出术。对术后患者治疗情况进行评价。结果：98%患者取得显著疗效。结论：可回收支架扩张治疗术具有简便、安全和疗效好等优点,并发症少,可作为贲门失弛缓症的首选治疗方法。     

Reduction of In-Stent Restenosis Risk on Nickel-Free Stainless Steel by Regulating Cell Apoptosis and Cell Cycle

High nitrogen nickel-free austenitic stainless steel (HNNF SS) is one of the biomaterials developed recently for circumventing the in-stent restenosis (ISR) in coronary stent applications. To understand the ISR-resistance mechanism, we have conducted a comparative study of cellular and molecular responses of human umbilical vein endothelial cells (HUVECs) to HNNF SS and 316L SS (nickel-containing austenitic 316L stainless steel) which is the stent material used currently. CCK-8 analysis and flow cytometric analysis were used to assess the cellular responses (proliferation, apoptosis, and cell cycle), and quantitative real-time PCR (qRT-PCR) was used to analyze the gene expression profile of HUVECs exposed to HNNF SS and 316L SS, respectively. Flow cytometry analysis revealed that 316L SS could activate the cellular apoptosis more efficiently and initiate an earlier entry into the S-phase of cell cycle than HNNF SS. At the molecular level, qRT-PCR results showed that the genes regulating cell apoptosis and autophagy were overexpressed on 316L SS. Further examination indicated that nickel released from 316L SS triggered the cell apoptosis via Fas-Caspase8-Caspase3 exogenous pathway. These molecular mechanisms of HUVECs present a good model for elucidating the observed cellular responses. The findings in this study furnish valuable information for understanding the mechanism of ISR-resistance on the cellular and molecular basis as well as for developing new biomedical materials for stent applications.     

铅污染现状及其修复机理研究进展

介绍了铅污染的现状、修复机理及存在的问题。其中重点阐述了国内在寻找铅的超累积植物的研究进展,国外研究者对铅在植物体内吸收、运输和储存机理方面的研究现状,同时对已发现的与铅污染有关的基因以及转基因技术的应用情况进行了简单介绍,并探讨了提高铅污染修复效率的一些方法。     

木耳属真菌rDNA特异性扩增片段的RFLP研究

对木耳属8个种25个菌株的ITS和28SrDNA5’端两个区域分别进行了PCR扩增和限制酶切片段长度多态性（RFLP）研究。ITS－RFMP研究结果表明,HaeⅢ可将黑木耳与其它种区分开,MspⅠ可将盾形木耳、角质木耳、琥珀木耳和黑木耳4个种区分开,而供试的HaeⅢ、TaqⅠ、HinfⅠ和MaPⅠ这四种限制酶均不能将皱木耳、大木耳、网脉木耳及毛木耳4个种区分开,表明它们之间的亲缘关系较近;结果还表明,ITS—rDNA拷贝在毛木耳和琥珀木耳种内是异质性的,而在黑木耳种内是同质性的。285rDNA-RFLP研究结果表明,供试的4种限制酶中,仅MspⅠ可将盾形木耳和角质木耳区分开,而不能将其它种区分开,这显示了28SrDNA序列在木耳属不同种间的保守性。     

稀有濒危植物翅果油树种群黄酮类化合物多样性研究

以稀有濒危植物翅果油树叶中黄酮类化合物为标记,探索翅果油树种群与其生境的相互关系。通过对濒危植物翅果油树的主要分布区,山西省的乡宁、翼城、平陆等三种群及环境的调查,并采集三个种群的24个样品。用水提法、甲醇提法,经聚酰胺薄层层析法分离,并将层析结果作1/0的二元数据化处理,用于聚类分析和PCA排序。结果表明:1)翅果油树叶中黄酮类化合物的种类比较稳定,可以作为研究种群分化的标记物;2)聚类分析表明乡宁、翼城、平陆三个种群间所含的黄酮类化合物已有分化,乡宁种群与翼城种群之间的相异性较小,在截取值为20时,乡宁与翼城种群首先聚为一支,当截取值为25时,平陆种群归并,表明平陆种群与其他种群的相异性较大;3)各种群内个体间的共享率有明显的差异4)PCA分析表明:不同种群内的黄酮类化合物的差异与其地理位置、温度、地形地貌及土壤养分等因子有关。     

三株亚肉座菌乙酸乙酯提取物生物活性及GC-MS分析

采用乙酸乙酯提取3株亚肉座菌菌丝体,测试虫生真菌乙酸乙酯提取物（EAE）的抗肿瘤、抗菌和抗氧化活性,并借助GC-MS方法分析各提取物中的化学成分。结果发现2株亚肉座菌的菌丝体EAE对HepG2细胞的抑制活性较强,IC₅₀均小于9μmol/L;抗菌结果表明2株真菌的提取物具有抑制细菌生长的作用;1株供试菌的EAE表现出较强的DPPH自由基清除活性（清除率可达85%）。GC-MS分析表明从亚肉座菌JXJG201717、JXJG201720和ARSEF7697的EAE中分别鉴定出21、35和39种成分,主要成分为酯类、醇类和酸类;盘状亚肉座菌JXJG201720与ARSEF7697有相同化合物13个,与暹罗亚肉座菌JXJG201717存在7个相同化合物。本研究表明虫生亚肉座菌具有产生丰富活性成分的能力,彰显出多种利用价值。     

混合蛋白质体系中魟鱼和海兔肝铁蛋白释放铁的动力学研究

分别制备含有魟鱼肝铁蛋白(1iver ferritin of Dasyatis akajei,DALF)和海兔肝铁蛋白(Liver ferritin of Aplysia,ALF)的混合蛋白质体系。选用电子光谱技术和不同电子供体,研究在混合蛋白质体系中,DALF和ALF释放铁的动力学过程和规律。实验结果表明,采用Na2S2O4作为还原剂时,DALF以两相行为进行释放铁的反应;而选用抗坏血酸作为还原剂时,DALF却以一级反应动力学方式进行释放铁的反应,简化释放铁的过程。在混合蛋白质体系中且以抗坏血酸和Na2S2O4为电子供体时,ALF均以一级反应动力学过程进行释放铁的反应,认为某些蛋白质参与协助ALF释放铁反应,从而简化释放铁的过程。     

Expression and characterization of the chitinases from Serratia marcescens GEI strain for the control of Varroa destructor, a honey bee parasite

Serratia marcescens GEI strain was isolated from the gut of the workers of Chinese honey bee Apis cerana and evaluated in the laboratory for the control of Varroa destructor, a parasite of western honey bee A. mellifera. The supernatant and the collected proteins by ammonium sulfate from the bacterial cultures showed a strong miticidal effect on the female mites, with 100% mite mortality in 5 days. Heat (100 °C for 10 min) and proteinase K treatment of the collected proteins destroyed the miticidal activity. The improved miticial activity of this bacterial strain on chitin medium indicated the involvement of chitinases. The expressed chitinases ChiA, ChiB and ChiC1 from S. marcescens GEI by recombinant Escherichia coli showed pathogenicity against the mites in the laboratory. These chitinases were active in a broad pH range (5-9) and the optimum temperatures were between 60 and 75 °C. Synergistic effects of ChiA and ChiB on the miticidal activity against V. destructor were observed. The workers of both honey bee species were not sensitive to the spraying and feeding chitinases. These results provided alternative control strategies for Varroa mites, by formulating chitinase agents and by constructing transgenetic honey bees.     

宁虫素对蔬菜、水稻害虫的毒力及药效特性研究

本研究了复方生物农药宁虫素对蔬菜、水稻害虫的毒力及药效特性,结果表明,该药集速效性、特效性于一体,对蚜虫、褐飞虱的毒力分别为：0.63ppm和2.00ppm。田间试验表明,宁虫素可有效地控制蔬菜蚜虫、小菜蛾、菜青虫、稻飞虱等的危害,防效显。田间使用宁虫素后,平均蛛虱比明显优于噻嗪酮和吡虫啉,生态安全性好。