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111.
The available burrow densities of plateau pika (Ochotona curzoniae) regulate the soil physicochemical property in alpine meadow. A field survey was conducted to investigate the effect of available burrow densities of plateau pika on soil physicochemical property of bare land (bare patch produced by burrowing behavior of plateau pika) and vegetation land (land covered with vegetation). This study indicated that the increase in available burrow of plateau pika caused the soil water content at 0–10 cm layer of bare land and that at 10–20 cm of vegetation land to reduce, and caused the soil water content at 10– 20 cm layer of bare land and that at 0–10 cm layer of vegetation land to firstly increase and then decline. In the increasing process of available burrow of plateau pika, the soil silt content firstly increased and then decreased, and soil sand content firstly decreased and then increased. With the increase of available burrow of plateau pika, the soil porosity at 0–10 cm layer of bare land and that at 10–20 cm layer of vegetation land decreased, while the soil porosity at 10–20 cm layer of bare land and that at 0–10 cm of vegetation land firstly increased and then decreased. Soil pH value, soil organic matter, total nitrogen and total phosphorus content firstly increased and then decreased, peaking at 14 available burrows per 625 m2, while total soil potassium content did not respond to available burrow densities of plateau pika. This study suggested that the proper available burrows existing in the alpine meadow increased soil permeability, accelerated soil moisture to penetrate deeply, increased the proportion of soil silt, and improved the soil nutrient; however, this beneficial effect was strongly influenced by the available burrow density of plateau pika, implying that plateau pika did not benefit soil structure when its available burrow was over 34 number/625 m2. 相似文献
112.
Wong KA Wilson J Russo A Wang L Okur MN Wang X Martin NP Scappini E Carnegie GK O'Bryan JP 《PloS one》2012,7(4):e36023
Members of the intersectin (ITSN) family of scaffold proteins consist of multiple modular domains, each with distinct ligand preferences. Although ITSNs were initially implicated in the regulation of endocytosis, subsequent studies have revealed a more complex role for these scaffold proteins in regulation of additional biochemical pathways. In this study, we performed a high throughput yeast two-hybrid screen to identify additional pathways regulated by these scaffolds. Although several known ITSN binding partners were identified, we isolated more than 100 new targets for the two mammalian ITSN proteins, ITSN1 and ITSN2. We present the characterization of several of these new targets which implicate ITSNs in the regulation of the Rab and Arf GTPase pathways as well as regulation of the disrupted in schizophrenia 1 (DISC1) interactome. In addition, we demonstrate that ITSN proteins form homomeric and heteromeric complexes with each other revealing an added level of complexity in the function of these evolutionarily conserved scaffolds. 相似文献
113.
Inhibition of p-glycoprotein under hyperglycemic conditions has been reported in various barrier tissues including blood-brain barrier, intestine, and kidney, and has been linked to significant clinical complications. However, whether this is also true for the outer blood-retinal barrier constituted by retinal pigment epithelium, or has a role in pathogenesis of diabetic retinopathy is not yet clear. In this study, using cultured human retinal pigment epithelium cell line D407, we found that high glucose exposure induced a significant decrease in p-glycoprotein expression both at mRNA and at protein levels, accompanied by an attenuated p-glycoprotein activity determined by intracellular rhodamine 123 retention. In marked contrast, the expressions of both mRNA and protein levels of inducible nitrate oxide synthase (iNOS) increased, and were accompanied by increased extracellular nitrate/nitrite production by Griess reaction. In addition, mRNA levels of nuclear receptors revealed a decreased expression of pregnane X receptor after the exposure of high glucose. However, the subsequent alterations in production of nitrate/nitrite, functional expression of p-glycoprotein, and mRNA levels of pregnane X receptor were partially blocked when pretreated with S,S′-1,3-phenylene-bis(1,2-ethanediyl)-bis-isothiourea•2HBr (PBITU), a selective iNOS inhibitor. Moreover, the effects of PBITU were antagonized with the addition of L-arginine, a substrate for NO synthesis. Our in vitro results suggest for the first time that iNOS induction plays a novel role in decreased p-glycoprotein expression and transport function at the human outer blood-retinal barrier under hyperglycemic conditions and further support the concept of inhibiting iNOS pathway as a therapeutic strategy for diabetic retinopathy. 相似文献
114.
Huang L Hu Y Huang Y Fang H Li R Hu D Li W Li X Liang C Yu X 《Molecular and cellular biochemistry》2012,363(1-2):367-376
Clonorchis sinensis fatty acid-binding protein (CsFABP) belongs to a multigene family of lipid-binding proteins and is considered to be a promising vaccine candidate for human clonorchiasis. In this study, binding characteristics of CsFABP have been examined for the first time. The recombinant CsFABP (rCsFABP) was found to bind 11-(dansylamino) undecanoic acid (DAUDA), causing a blue shift in the fluorescence emission from 543 to 531 nm with an excitation wavelength of 345 nm and a substantial increase in fluorescence intensity. Fluorimetric titration of rCsFABP with DAUDA exhibited an apparent dissociation constant (K (d)) of 1.58 ± 0.14 μM. In the competitive experiment, the rCsFABP efficiently bound saturated C(10)-C(18) fatty acids and unsaturated fatty acids (oleic acid and linoleic acid), and the latter presented the higher affinity. Furthermore, quantitative RT-PCR and western blotting analysis revealed that CsFABP mRNA and protein were differentially expressed throughout the developmental cycle stages of the parasite, which occur in the definitive host (metacercariae, adult worms, and eggs). In addition, immunolocalization assay showed that CsFABP was localized on the vitelline gland, tegument, intestine, seminal vesicle, eggs in uterus, ovary, and testicle of C. sinensis adult worm, as well as on the vitelline gland of metacercaria. Intriguingly, the surface tissue of the bile duct where C. sinensis resided in the infected Sprague-Dawley rat was also strongly labeled, implying that CsFABP may possibly mediate direct interactions with host cells as a component of excretory/secretory products. 相似文献
115.
Kariuki MM Li X Yamodo I Chishti AH Oh SS 《Biochemical and biophysical research communications》2005,338(4):1690-1695
Erythrocyte invasion by malaria parasites requires multiple protein interactions. Our earlier studies showed that erythrocyte band 3 is an invasion receptor binding Plasmodium falciparum merozoite surface protein 1 and 9 (MSP1, MSP9) existing as a co-ligand complex. In this study, we have used biochemical approaches to identify the binding sites within MSP1 and MSP9 involved in the co-ligand complex formation. A major MSP9-binding site is located within the 19kDa C-terminal domain of MSP1 (MSP1(19)). Two specific regions of MSP9 defined as Delta1a and Delta2 interacted with native MSP1(19). The 42 kDa domain of MSP1 (MSP1(42)) bearing MSP1(19) in the C-terminus bound directly to both MSP9/Delta1a and Delta2. Thus, the regions of MSP1 and MSP9 interacting with the erythrocyte band 3 receptor are also responsible for assembling the co-ligand complex. Our evidence suggests a ternary complex is formed between MSP1, MSP9, and band 3 during erythrocyte invasion by P. falciparum. 相似文献
116.
Ying Yuan Shengjie Tang Yuan Li Dongmei Yan Qunxin Peng Xuerong Ya Yanhui Song 《中国病毒学》2018,33(2):209-212
正Dear Editor,Human immunodeficiency virus(HIV)and acquired immunodeficiency syndrome(AIDS)remain major public health problems in China,where approximately 780,000people currently live with HIV,according to the 2012 China AIDS Response Progress Report.Suzhou was an early epidemic region in Jiangsu province and the current situation of 相似文献
117.
本文以水稻为材料,研究化学诱变剂dES、NaN_3对种子萌发及幼苗生长发育的影响。用不同浓度的dES、NaN_3处理预浸泡的水稻种子,测定种子的发芽率、发芽势、幼苗高度和根系活力,同时测定其呼吸强度和细胞色素氧化酶活性。结果表明,dES、NaN_3对水稻初期生长发育具有抑制作用。种子经化学诱变剂dES或NaN_3处理后,细胞呼吸强度和细胞色素氧化酶活性明显下降,种子发芽率、发芽势、幼苗生长高度和根系活力也不同程度地降低。诱变剂的剂量愈高,抑制愈强烈。由试验结果可知,化学诱变剂首先影响细胞的能量代谢活动,从而导致种子发芽率降低,幼苗生长迟缓,根系活力下降。 相似文献
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