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101.
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103.
Xiao Yang Dai Yaohong Lu Yingying Liu Wei Wang Zhuoren Bian Yinbing 《World journal of microbiology & biotechnology》2011,27(7):1731-1734
Rapid and correct authentication of commercial strains is still important in today’s mushroom industry. Here for the first
time we reported the using of sequence characterized amplified region (SCAR) marker developed from inter-retrotransposon amplified
polymorphism (IRAP) marker to identify Lentinula edodes strain. Genomic polymorphism among 44 shiitake cultivars in China were surveyed by 24 IRAP primer combinations, from which
primer combination LTR1L/MarY1R could generate a unique and reproducible 1712 bp fragment to distinguish strain No. 4 from
other 43 strains. Based on this strain-specific fragment, a SCAR primer pair was designed and its validity was verified by
correctly amplifying a single strain-specific fragment from DNA samples of 100 L. edodes strains. Our study lays the foundation for developing strain-specific SCAR marker by retrotransposon-based marker technique
in fungi. 相似文献
104.
Bian Lei Molan Abdul-Lateef Maddox Ian Shu Quan 《World journal of microbiology & biotechnology》2011,27(4):991-998
Lactobacillus reuteri DPC16 is a human-isolated strain recently patented in New Zealand. The antimicrobial activity of cell-free supernatants from
different fermentation processes, with or without glycerol supplementation was studied. When grown in just MRS broth, the
cultural supernatant significantly inhibited the growth of selected food-borne pathogens, possibly due to acidic effect as
this activity was pH-dependent. The cell-free supernatants from secondary fermentation of DPC16 resting cells in glycerol-supplemented
media have shown very different antimicrobial activities. A very potent antimicrobial activity gradually developed during
the fermentation process which was observed only when growing in MRS-glycerol broth (such supernatant is denoted MRSg). This
strong antimicrobial activity was pH-independent, dose-dependant and affected both Gram-negative and Gram-positive pathogens.
Reuterin detected in MRSg is believed to be responsible for these activities. The susceptibility of the selected pathogens
(grown to stationary phase) to MRSg was tested and found that exposure to MRSg for 180 min led to a significant reduction
in cell viability in all pathogens. These results suggest that this is a reuterin-producing strain, which has potent antimicrobial
activity against both Gram-negative and Gram-positive pathogens. These findings have indicated a clear potential of this novel
strain in industrial applications. 相似文献
105.
Li Li Xiu-Zhi Fan Wei Liu Yang Xiao Yin-Bing Bian 《World journal of microbiology & biotechnology》2011,27(9):2081-2093
Phenotypic traits (physiological characteristics and somatic incompatibility) and genotypic traits (target region amplification
polymorphism TRAP) were used to study the diversity of 32 main cultivars of Auricularia auricula-judae in China. Twenty seven important and stable physiological indexes were evaluated; somatic incompatibility test (SIT) reaction
was described from three aspects: type, pigment, and intensity; 16 pairs of TRAP primer combinations produced 535 unambiguous
and reproducible DNA fragments, of these 524 (97.9%) were polymorphic. Dendrograms were constructed by Unweighted Pair-group
Method with Arithmetic Averages (UPGMA) method, and the principal coordinate analysis (PCO) of the three methods (physiological
characteristics, SIT intensity and TRAP) exhibited similar clustered patterns, revealing that all the tested strains could
be divided into three distinct groups, each of which was correlated with different geographical regions. Most strains originated
from the same area were with a narrow genetic basis and could possibly be domesticated from the local wild-type strains, some
strains were suspected to be synonymous. The grouping information obtained in the present work provides significant information
for further genetic improvement of A. auricula-judae. 相似文献
106.
107.
Chunxia Zhang Mingdi Bian Hui Yu Qing Liu Zhenming Yang 《Plant Physiology and Biochemistry》2011,49(11):1306-1312
Calcineurin B-like proteins play important roles in the calcium perception and signal transduction of abiotic stress. In this study, the bioinformatic analysis of molecular characteristics of Sorghum bicolor calcineurin B-like protein (SbCBL) revealed that sequences of SbCBL are highly conserved, and most SbCBLs have three typical EF-hands structures. Among the SbCBL proteins, four of which, SbCBL01, 04, 05, 08, have a conserved N-myristoylation domain. Stress-responsive and phytohormone-responsive cis-elements were found in the promoter regions of SbCBL genes. Real-time quantitative polymerase chain reaction (RTqPCR) analysis showed that SbCBL genes have different tissue-specific expression patterns under normal growth conditions in sweet sorghum (Sorghum bicolor L. Moench). Interestingly, when treated with sodium carbonate, SbCBL genes also show various sodium carbonate stress responsive patterns in sweet sorghum seedlings. These results suggest that SbCBLs may participate in regulating sodium carbonate stress-specific cellular adaptation responses and influencing growth and developmental patterns in sweet sorghum. 相似文献
108.
A slow infiltration experiment with different hydraulic loads (0, 3, 6, 9, 12, and 15 cm per week) of domestic wastewater was conducted in a 'Zhonglin 2001' poplar plantation to study the effects of the wastewater slow infiltration on the growth of the plantation. Comparing with the control (0 cm), the other five treatments increased the soil organic matter, total N, total P, total K, and Na+ contents in the plantation averagely by 1.940 g x kg(-1), 0.115 g x kg(-1), 0.029 g x kg(-1), 1.454 g x kg(-1) and 0.030 g x kg(-1), respectively. At lower hydraulic loads (3-12 cm per week), the poplar biomass growth and the N, P and Na+ contents in different poplar organs averagely increased by 17.583 t x hm(-2) x a(-1), 3.086 g x kg(-1), 0.645 g x kg(-1), and 0.121 g x kg(-1), with the maximum (36.252 t x hm(-2) x a(-1), 13.162 g x kg(-1), 5.137 g x kg(-1), and 0.361 g x kg(-1), respectively) at hydraulic loads 6-12 cm per week. The further increase of the hydraulic load decreased the poplar biomass growth and the N, P and Na+ contents in different poplar organs. The K content in different poplar organs decreased with increasing hydraulic load. Treating with domestic wastewater increased the leaf length, decreased the leaf asymmetry, and delayed leaf-falling. At high hydraulic load (15 cm per week), the higher soil Na+ and water contents would threat the poplar growth. The proper domestic wastewater hydraulic loads for the growth of poplar 'Zhonglin 2001' plantation would be 3-12 cm per week. 相似文献
109.
A new spectrofluorimetric method was developed for determination of trace amount of bilirubin. Using oxytetracycline–Eu3+ as a fluorescent probe, in the buffer solution of pH = 7.3, bilirubin can reduce remarkably the fluorescence intensity of the oxytetracycline–Eu3+ complex at λ = 612 nm and the reduced fluorescence intensity was in proportion to the concentration of bilirubin. Optimum conditions for the determination of bilirubin were also investigated. The linear range and limit of detection for the determination of bilirubin were 5.0 × 10?7, 3.0 × 10?5 and 7.7 × 10?8 mol L?1, respectively. This method is simple, practical and relatively free of interference from coexisting substances and can be successfully applied to assess bilirubin in serum samples and compared with the modified Jendrassik–Grof method in clinical analysis. The quenching mechanism of the fluorescence intensity in the system is also discussed. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
110.
Yi Kong Tianhua Yan Feifei Feng Jianmin Bian Yan Yang Haining Yu 《Journal of peptide science》2011,17(7):540-544
The defensin‐like antimicrobial peptides have been characterized from various other arthropods including insects, scorpions, and ticks. But no natural spider defensin‐like antimicrobial peptides have ever been isolated from spiders, except couple of cDNA and DNA sequences of five spider species revealed by previous genomic study. In this work, a defensin‐like antimicrobial peptide named Oh‐defensin was purified and characterized from the venoms of the spider, Ornithoctonus hainana. Oh‐defensin is composed of 52 amino acid (aa) residues including six Cys residues that possibly form three disulfide bridges. Its aa sequence is MLCKLSMFGAVLGV PACAIDCLPMGKTGGSCEGGVCGCRKLTFKILWDKKFG. By BLAST search, Oh‐defensin showed significant sequence similarity to other arthropod antimicrobial peptides of the defensin family. Oh‐defensin exerted potent antimicrobial activities against tested microorganisms including Gram‐positive bacteria, Gram‐negative bacteria, and fungi. The cDNA encoding Oh‐defensin precursor was also cloned from the cDNA library of O. hainana. Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd. 相似文献