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81.
Ashwani Kumar Galina Aglyamova Yun
Young Yim Aaron O Bailey Haley
M Lynch Reid
T Powell Nghi
D Nguyen Zachary Rosenthal Wen-Ning Zhao Yi Li Jianping Chen Shanghua Fan Hubert Lee William
K Russell Clifford Stephan Alfred
J Robison Stephen
J Haggarty Eric
J Nestler Jia Zhou Mischa Machius Gabby Rudenko 《Nucleic acids research》2022,50(16):9548
82.
Wei Lv Wei Jiang Hongmei Luo Qian Tong Xiaoyu Niu Xiao Liu Yang Miao Jingnan Wang Yiwen Guo Jianan Li Xizhen Zhan Yunqing Hou Yaxin Peng Jian Wang Shuhong Zhao Zaiyan Xu Bo Zuo 《Nucleic acids research》2022,50(18):10733
Long noncoding RNAs (lncRNAs) play important roles in the spatial and temporal regulation of muscle development and regeneration. Nevertheless, the determination of their biological functions and mechanisms underlying muscle regeneration remains challenging. Here, we identified a lncRNA named lncMREF (lncRNA muscle regeneration enhancement factor) as a conserved positive regulator of muscle regeneration among mice, pigs and humans. Functional studies demonstrated that lncMREF, which is mainly expressed in differentiated muscle satellite cells, promotes myogenic differentiation and muscle regeneration. Mechanistically, lncMREF interacts with Smarca5 to promote chromatin accessibility when muscle satellite cells are activated and start to differentiate, thereby facilitating genomic binding of p300/CBP/H3K27ac to upregulate the expression of myogenic regulators, such as MyoD and cell differentiation. Our results unravel a novel temporal-specific epigenetic regulation during muscle regeneration and reveal that lncMREF/Smarca5-mediated epigenetic programming is responsible for muscle cell differentiation, which provides new insights into the regulatory mechanism of muscle regeneration. 相似文献
83.
Bo Li YuZhen Ge WeiWei Yan Bin Gong Kun Cao Rui Zhao Chao Li YeWei Zhang YiHeng Jiang Shi Zuo 《Cell proliferation》2022,55(9)
As a member of the deoxyribonuclease 1 family, DNASE1L3 plays a significant role both inside and outside the cell. However, the role of DNASE1L3 in hepatocellular carcinoma (HCC) and its molecular basis remains to be further investigated. In this study, we report that DNASE1L3 is downregulated in clinical HCC samples and evaluate the relationship between its expression and HCC clinical features. In vivo and in vitro experiments showed that DNASE1L3 negatively regulates the proliferation, invasion and metastasis of HCC cells. Mechanistic studies showed that DNASE1L3 recruits components of the cytoplasmic β‐catenin destruction complex (GSK‐3β and Axin), promotes the ubiquitination degradation of β‐catenin, and inhibits its nuclear transfer, thus, decreasing c‐Myc, P21 and P27 level. Ultimately, cell cycle and EMT signals are restrained. In general, this study provides new insight into the mechanism for HCC and suggests that DNASE1L3 can become a considerable target for HCC.Decreased expression of DNASE1L3 is associated with poor prognosis in patients with HCC DNASE1L3 inhibits the proliferation and cell cycle of HCC cells in vitro and promotes the invasion and metastasis of HCC cells DNASE1L3 inhibits the tumorigenicity and metastasis of HCC cells in vivo DNASE1L3 interacts with β‐catenin and promotes its binding to the β‐catenin destroying complex DNASE1L3 interacts with P21 and stabilizes P21 by mediating the deubiquitin activity 相似文献
84.
BackgroundPlanarian has attracted increasing attentions in the regeneration field for its usefulness as an important biological model organism attributing to its strong regeneration ability. Both the complexity of multiple regulatory networks and their coordinate functions contribute to the maintenance of normal cellular homeostasis and the process of regeneration in planarian. The polarity, size, location and number of regeneration tissues are regulated by diverse mechanisms. In this review we summarize the recent advances about the importance genetic and molecular mechanisms for regeneration control on various tissues in planarian.MethodsA comprehensive literature search of original articles published in recent years was performed in regards to the molecular mechanism of each cell types during the planarian regeneration, including neoblast, nerve system, eye spot, excretory system and epidermal.ResultsAvailable molecular mechanisms gave us an overview of regeneration process in every tissue. The sense of injuries and initiation of regeneration is regulated by diverse genes like follistatin and ERK signaling. The Neoblasts differentiate into tissue progenitors under the regulation of genes such as egfr‐3. The regeneration polarity is controlled by Wnt pathway, BMP pathway and bioelectric signals. The neoblast within the blastema differentiate into desired cell types and regenerate the missing tissues. Those tissue specific genes regulate the tissue progenitor cells to differentiate into desired cell types to complete the regeneration process.ConclusionAll tissue types in planarian participate in the regeneration process regulated by distinct molecular factors and cellular signaling pathways. The neoblasts play vital roles in tissue regeneration and morphology maintenance. These studies provide new insights into the molecular mechanisms for regulating planarian regeneration. Genetic and molecular mechanisms for regeneration control on various tissues in planarian. 相似文献
85.
86.
Wei Zhao Lan Yao Min Zhuang Yuan-Long Lin Xiao-Hong Chen Li Wang Bo Song Ya-Shuang Zhao Yun Xiao Feng-Min Zhang Fu-Xiang Wang Hong Ling 《PLoS neglected tropical diseases》2022,16(9)
BackgroundHuman immunodeficiency virus (HIV) and enteric parasite co-infection not only aggravates the clinical symptoms of parasites but also accelerates acquired immunodeficiency syndrome (AIDS) progression. However, co-infection research on men who have sex with men (MSM), the predominant high-risk population of HIV/AIDS in China, is still limited. In this study, we investigated the epidemiology of enteric parasites, risk factors, and associations with clinical significance in an MSM HIV/AIDS population in Heilongjiang Province, northeast China.MethodsWe recruited 308 MSMs HIV/AIDS patients and 199 HIV-negative individuals in two designated AIDS hospitals in Heilongjiang between April 2016 and July 2017. Fresh stool samples were collected. DNA extraction, molecular identification, and genotyping of Cryptosporidium species, Entamoeba histolytica, Cyclospora cayetanensis, Enterocytozoon bieneusi, and Blastocystis hominis were performed. Fourteen diarrhea-related pathogens were examined to exclude the influence of other bacterial pathogens on diarrhea incidence.Results31.5% of MSM HIV/AIDS participants were infected with at least one parasite species, a significantly higher proportion than that found in the HIV-negative individuals (2.5%). E. bieneusi presented the highest prevalence, followed by B. hominis, E. histolytica, Cryptosporidium spp., and C. cayetanensis. Warm seasons were the risk factor for parasitic infections in this population [odds ratio (OR) = 2.6, 95% CI: 1.47–4.57]. In addition, these individuals showed a higher proportion (35.8%) of present diarrhea (PD) compared with men who have sex with women (MSW) with HIV/AIDS (16.7%). The infection proportions of both Cryptosporidium spp. and E. histolytica were significantly higher in the PD. E. bieneusi infection was more prevalent in the historic diarrhea (HD) group. CD4+ T cell counts in the MSM patients with the above three parasites were significantly lower. New species and genotypes were found, and MSM patients had a wider range of species or genotypes.ConclusionsEnteric parasitic infection was prevalent in the MSM HIV/AIDS population, especially in patients with present diarrhea during warm seasons. E. histolytica and B. hominis should also be considered high-risk parasites for opportunistic infections in AIDS patients in addition to Cryptosporidium spp. 相似文献
87.
Yingxin Ma Guobin Mao Weishan Yang Guoqiang Wu Guoqiang Li Xiaoying Li Xin Lin Junnan Lu Shijun Zhao Wei Zhao Junbiao Dai Xian-En Zhang 《中国科学:生命科学英文版》2022,65(9):1894-1897
<正>Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARSCoV-2) was identified as the pathogen causing the coronavirus disease(COVID-19), which sometimes resulted in fatal pneumonia(Hu et al., 2021). SARS-CoV-2 is a biosafety level 3(BSL-3) pathogen, and the requirement for high containment conditions is a bottleneck for basic research on viral biology. 相似文献
88.
137Cs示踪技术研究坡耕地黑土侵蚀和沉积特征 总被引:21,自引:1,他引:20
准确地测定研究区137Cs背景值,建立137Cs流失量与土壤再分布速率之间的定量模型是137Cs示踪技术的关键。通过野外选择参照样地和利用热核爆炸源137Cs背景值模型来确定研究区137Cs的背景值,在此基础上用体现耕作迁移的质量平衡模型估算黑土坡耕地不同地貌部位的土壤再分布速率,并对主要参数进行敏感性分析。结果表明(1)研究区实测的137Cs背景值为2376.81±108.46Bq/m2,模型预测值为2318.4Bq/m2,模型预测远离西北核试验基地的地区较为准确。(2)研究区中坡位(坡肩和坡背)137Cs含量最低,侵蚀最为强烈,平均侵蚀速率为33.56t/(hm2·a)和21.67t/(hm2·a);坡麓和坡足则明显表现沉积,平均沉积速率为-4.93t/(hm2·a)和-24.61t/(hm2·a)。(3)模型预测的侵蚀速率与耕层质量深度(d)、张驰深度(H)正相关,而与137Cs年沉降易被迁移的比例(γ)和颗粒校正因子(P)反相关。并且,模型对参数d、p的敏感性分别高于参数H和γ。 相似文献
89.
90.
Minglong Liang Zongmei Wang Chuanjian Wu Sidong Xiong Ling Zhao Chunsheng Dong 《中国病毒学》2022,37(3):455-458
Highlights:
1. A replication-competent recombinant VSV with RABV-G protein replacement was generated.
2. Single dose of VSV-RABVG immunization induce potent antigen-specific humoral immune response, especially the virus neutralizing antibodies.
3. Mice intranasally immunized with single dose of VSV-RABVG were 100% protected upon RABV challenge. 相似文献
1. A replication-competent recombinant VSV with RABV-G protein replacement was generated.
2. Single dose of VSV-RABVG immunization induce potent antigen-specific humoral immune response, especially the virus neutralizing antibodies.
3. Mice intranasally immunized with single dose of VSV-RABVG were 100% protected upon RABV challenge. 相似文献