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121.
Characterization of streptomyces lydicus WYEC108 as a potential biocontrol agent against fungal root and seed rots. 总被引:2,自引:0,他引:2
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The actinomycete Streptomyces lydicus WYEC108 showed strong in vitro antagonism against various fungal plant pathogens in plate assays by producing extracellular antifungal metabolites. When Pythium ultimum or Rhizoctonia solani was grown in liquid medium with S. lydicus WYEC108, inhibition of growth of the fungi was observed. When WYEC108 spores or mycelia were used to coat pea seeds, the seeds were protected from invasion by P. ultimum in an oospore-enriched soil. While 100% of uncoated control seeds were infected by P. ultimum within 48 h after planting, less than 40% of coated seeds were infected. When the coated seeds were planted in soil 24 h prior to introduction of the pathogen, 96 h later, less than 30% of the germinating seeds were infected. Plant growth chamber studies were also carried out to test for plant growth effects and for suppression by S. lydicus WYEC108 of Pythium seed rot and root rot. When WYEC108 was applied as a spore-peat moss-sand formulation (10(8) CFU/g) to P. ultimum-infested sterile or nonsterile soil planted with pea and cotton seeds, significant increases in average plant stand, plant length, and plant weight were observed in both cases compared with untreated control plants grown in similar soils. WYEC108 hyphae colonized and were able to migrate downward with the root as it elongated. Over a period of 30 days, the population of WYEC108 colonized emerging roots of germinating seeds and remained stable (10(5) CFU/g) in the rhizosphere, whereas the nonrhizosphere population of WYEC108 declined at least 100-fold (from 10(5) to 10(3) or fewer CFU/g). The stability of the WYEC108 population incubated at 25 degrees C in the formulation, in sterile soil, and in nonsterile soil was also evaluated. In all three environments, the population of WYEC108 maintained its size for 90 days or more. When pea, cotton, and sweet corn seeds were placed into sterile and nonsterile soils containing 10(6) or more CFU of WYEC108 per g, it colonized the emerging roots. After a 1-week growing period, WYEC108 populations of 10(5) CFU/g (wet weight) of root were found on pea roots in the amended sterile soil environment versus 10(4) CFU/g in amended nonsterile soil. To further study the in vitro interaction between the streptomycete and P. ultimum, mycelia of WYEC108 were mixed with oospores of P. ultimum in agar, which was then used as a film to coat slide coverslips.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
122.
中枢ACTH受体研究进展 总被引:2,自引:0,他引:2
除垂体以外,中枢神经系统也含有促肾上腺皮质激素(ACTH)能神经元,其神经纤维在中枢具有较广泛的投射。ACTH相关肽类在中枢发挥着多种生理功能。近年来对于中枢ACTH受体的研究取得很大的进展,现已确认ACTH结合位点在中枢具有广泛的分布。新近克隆出的四种ACTH受体中,有两种是中枢神经系统占优势的受体亚型。 相似文献
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氧化应激与cfos和cjun转录和AP-1激活 总被引:2,自引:0,他引:2
激活剂蛋白-1(activator protein-1,AP-1)是近年来受到关注的与氧化应激基因表达调控有关的转录因子.文章就氧化应激与cfos和cjun基因表达,AP-1的激活,AP-1对氧化应激反应的调控,AP-1与亲电子反应元件等有关内容作了简要的综述. 相似文献
126.
我国自然保护区的生态旅游开发 总被引:42,自引:0,他引:42
我国自然保护区的生态旅游开发袁兴中,刘红,高天刚(山东曲阜师范大学273165)DevelopmentofEcotourisminNaturalReservesofChina¥YuanXingzhong;LiuHong;GaoTiangang(Quf... 相似文献
127.
用闪光诱导瞬间二向色性方法测量了蔗糖和甘油对菌紫质(BR)分子在脂质泡中的旋转扩散运动的影响。结果表明脂质囊泡本身在悬浮液中的运动并不会影响BR分子在膜中的旋转运动的测量。蔗糖和甘油对BR旋转运动的影响在相变温度以上和相变温度以下是不同的,相变温度以下的主要作用是相分离,使运动减慢,相变温度以上的作用可能是脂的分散,使运动加快。 相似文献
128.
G. Bechis F. Sampieri P-M. Yuan T. Brando M-F. Martin C.R. Diniz H Rochat 《Biochemical and biophysical research communications》1984,122(3):1146-1153
The sequence of the 61 amino acids of toxin VII, a β-toxin from the venom of the South American scorpion Tityus serrulatus, has been determined by automatic sequencing of the reduced and S-[14C] car?ymethylated protein and of tryptic peptides obtained before or after citraconylation of this protein. This toxin, the most active β-toxin from this venom, is the first Tityus toxin to be fully sequenced. The results clearly show that toxin VII belongs to the structural group of scorpion toxins originating from Central and North America. 相似文献
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